A5101599677- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Cancer, Lipids, and Metabolism
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Liver Disease Diagnosis and Treatment
- Listeria monocytogenes in Food Safety
- Metabolism, Diabetes, and Cancer
- Bacterial Genetics and Biotechnology
- Digestive system and related health
- Microbial Inactivation Methods
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Gut microbiota and health
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Escherichia coli research studies
- Advanced Breast Cancer Therapies
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
Sanya University
2025
Hainan University
2025
Oslo University Hospital
2024
University of Oslo
2024
Tianjin Medical University
2023
Wuhan University
2022
Renmin Hospital of Wuhan University
2022
Nanyang Normal University
2022
National Center for Agricultural Utilization Research
2010-2021
Kailuan General Hospital
2019-2021
ABSTRACT Listeria monocytogenes is a food-borne bacterial pathogen that able to grow at refrigeration temperatures. To investigate microbial gene expression associated with cold acclimation, we used differential cDNA cloning procedure known as selective capture of transcribed sequences (SCOTS) identify RNAs were expressed elevated levels in bacteria grown 10°C compared those 37°C. A total 24 different clones corresponding open reading frames the L. strain EGD-e genome obtained by SCOTS....
Summary Significant progress in apomictic hybrid rice development faces challenges like achieving high induction rates and seed‐setting efficiencies, distinguishing clonal from zygotic embryos. To address the challenge of selecting seeds, we developed a dual‐fluorescence labelling gene switch system using recombinase Cre/LoxP + FRT . Initially, this was tested callus tissue under constitutive promoter; then, replaced promoter with pollen‐specific one to develop (PSGS) system. The...
A Pd-catalyzed, ligand-free method for β,δ-selective consecutive arylation of internal alkenes with aryl iodides has been developed. This transformation employs a native group-directed double Heck arylation, utilizing selective β-H elimination...
ABSTRACT A cold-sensitive Listeria monocytogenes mutant designated cld-14 was obtained by transposon Tn 917 mutagenesis. The gene interrupted in the L. LMOf2365_ 1485 homolog, which exhibits 45.7% homology to Bacillus subtilis yqfF locus. , here pgpH encodes a putative integral membrane protein with predicted molecular mass of 81 kDa. PgpH is contain conserved N-terminal signal peptide sequence, seven transmembrane helices, and hydrophilic C terminus, likely extends into cytosol. insertion...
The influence of fasting blood glucose (FBG) and cholesterolemia primary liver cancer (PLC) in china was analyzed via a large prospective cohort study based on community population, the combined effects between them were investigated.Overall, 98,936 staff from Kailuan Group who participated finished physical examinations 2006 2007 included study. Their medical information collected they followed up after examination. correlations serum FBG or total cholesterol (TC) with PLC analyzed. Then,...
Abstract New methods of safe biological pest control are required as a result evolution insect resistance to current biopesticides. Yeast strains being developed for conversion cellulosic biomass ethanol potential host systems expression commercially valuable peptides, such bioinsecticides, increase the cost‐effectiveness process. Spider venom is one many sources novel insect‐specific peptide toxins. Libraries mutants small amphipathic lycotoxin‐1 from wolf spider were produced in high...
Lactobacillus brevis ATCC367 was engineered to express pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) genes in order increase ethanol fermentation from biomass-derived residues. First, a Gram-positive Sarcina ventriculi PDC gene (Svpdc) introduced into L. ATCC 367 obtain bbc03. The SvPDC detected by immunoblot using an oligo peptide antiserum, but no increased Then, ADH (Bradh) cloned behind the Svpdc that generated pdc/adh-coupled cassette pBBC04. pBBC04 restored anaerobic...
The commercial production of mannitol involves high-pressure hydrogenation fructose using a nickel catalyst, costly process. Mannitol can be produced through fermentation by microorganisms. Currently, few Lactobacillus strains are used to develop an efficient process for bioproduction; most the produce from with other products. An approach toward improving this would genetically engineer increase fructose-to-mannitol conversion decreased We cloned gene mtlK encoding mannitol-2-dehydrogenase...
Increased interest in sustainable production of renewable diesel and other valuable bioproducts is redoubling efforts to improve economic feasibility microbial-based oil production. Yarrowia lipolytica capable employing a wide variety substrates produce co-products. We irradiated Y. NRRL YB-567 with UV-C enhance ammonia (for fertilizer) lipid biodiesel) on low-cost protein carbohydrate substrates. The resulting strains were screened for using color intensity indicators plate assays. Seven...
The Lactobacillus buchneri NRRL B-30929 strain, isolated from a fuel ethanol (EtOH) production facility, exhibits high tolerance to environmental EtOH concentrations. This study aimed identify proteins produced by in response EtOH. Cellular expressed growing media with 10 versus 0% were compared 2DE, followed in-gel digestion and MALDI-MS analyses. Twenty responsive identified. These include proline-specific peptidase (Lbuc_1852); membrane protein (Lbuc_0921), two general stress-related...
Robotic platforms are essential for production of large numbers expression-ready plasmid sets to develop optimized clones and improved microbial strains crucial bioenergy applications simultaneous high-value peptide expression. Here we demonstrate a plasmid-based integrated robotic workcell, modified with motorized vacuum filtration system, performing fully automated molecular biology protocols, including assembly mutagenized gene sequences, purification PCR amplicons, ligation products into...
This study reports the production and characterization of a novel antibacterial polypeptide, designated laparaxin, which is secreted by Lactobacillus paracasei NRRL B-50314. Crude laparaxin has activity against wide variety Gram-positive bacteria, including: lactic acid bacteria (Lactococcus lactis buchneri), food-borne pathogens (Listeria monocytogenes), gastrointestinal (Enterococcus faecalis), opportunistic (Staphylococcus aureus methicillin-sensitive (MSSA) methicillin resistant (MRSA)...
Abstract Background Although a high‐salt diet is associated with high risk of chronic diseases such as hypertension, stroke and cardiovascular disease, little known about the relationship between primary liver cancer (PLC). Consequently, we prospectively assessed association perceived salt intake PLC in Kailuan Study. Methods In total, 97 006 participants who were healthy adults or free living at baseline (2006) included present study. The data collected via questionnaire classified into...
Previous studies have shown that serum total cholesterol (TC) and alanine aminotransferase (ALT) are associated with liver cancer risk. However, the common contribution of TC normal-high ALT to primary (PLC) has not been reported. We aim assess separate joint effect low level on risk PLC, a large prospective cohort was conducted in our study.The participants were divided into 4 groups via cross-matching method according [low (-)/non-low (+)] [normal (-)/normal-high level(+)] status, using...