A5030064571- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Enzyme Production and Characterization
- Catalysis for Biomass Conversion
- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Protein Hydrolysis and Bioactive Peptides
- Gut microbiota and health
- Polysaccharides Composition and Applications
- Proteins in Food Systems
United States Department of Agriculture
2009-2023
Agricultural Research Service
2009-2023
National Center for Agricultural Utilization Research
2009-2023
Worldwide, there are various feedstocks such as straws, corn stover, sugarcane bagasse, sweet sorghum bagasse (SSB), grasses, leaves, whey permeate, household organic waste, and food waste (FW) that can be converted to valuable biofuels butanol. For the present studies, an economic analysis was performed compare butanol production from three (SSB; FW; yellow top presscake, YTP or YT) using a standard process advanced integrated design. The total plant capacity set at 170,000–171,000 metric...
This study applied concentrated xylose (60–250 g/L) medium to produce butanol (acetone ethanol, or ABE). A control batch fermentation of 61 g/L initial glucose using Clostridium beijerinckii P260 resulted in a productivity and yield 0.33 g/L·h 0.43 g/g, respectively. Use 60 system 0.26 g/L·h, 0.40 In these two experiments, the culture fermented 89.3% 83.6% xylose, When ABE recovery was coupled with for continuous solvent removal, all added (60 g/L). 0.66 0.44 sugar concentration further...
A Lactobacillus buchneri strain NRRL B-30929 can convert xylose and glucose into ethanol chemicals. The aims of the study were to survey three strains (NRRL B-30929, 1837 DSM 5987) for fermenting 17 single substrates exam mixed from biomass hydrolysates.Mixed acid fermentation was observed all L. using various carbohydrates; only exception uridine which yielded lactate, acetate uracil. Only is capable utilizing cellobiose, a desired trait in potential biocatalyst conversion. Flask indicated...
Abstract In these studies, concentrated xylose solution was fermented to ethanol using Escherichia coli FBR5 which can ferment both lignocellulosic sugars (hexoses and pentoses). E. produce 40–50 g L −1 from 100 in batch reactors. Increasing sugar concentration beyond this level results the loss of with reactor effluent thus affecting process yield adversely. a nonintegrated system without simultaneous product removal more than 120 left unused 220 that fed into reactor. contrast this,...
Butanol was produced commercially from cornstarch and sugarcane molasses (renewable resources) until 1983, when production of these plants forced to cease because unfavorable economics caused in part by escalating prices feedstocks. During recent years, the focus research has been on use economically available agricultural biomass residues cutting-edge science technology make butanol a viable process again. In this study, we sweet sorghum bagasse (SSB) employing high concentrations SSB...
Abstract Five reactor systems (free cell batch, free continuous, entrapped immobilized, adsorbed packed bed, and recycle membrane reactors) were compared for ethanol production from xylose using Escherichia coli FBR5. In the batch continuous reactors (continuous stirred tank reactor‐CSTR) productivities of 0.84 gL −1 h 1.77 achieved, respectively. A resulted in highest productivity 55.56 , which is an increase 66‐fold (e.g., 6614%) over reactor. Calcium alginate gel CSTR a 2.04 whereas bed...
Butanol can be produced biologically through fermentation of lignocellulosic biomass-derived sugars by Gram-positive Clostridium species. For cost-effective production, increased butanol titers are desired. However, the currently available butanol-fermenting microbes do not tolerate sufficiently high concentrations; thus, new butanol-tolerant strains One promising strategy is to genetically modify species introducing stress tolerance-associated genes. This study was aimed seek tolerance...
In this study, the ability of a novel strain Clostridium tyrobutyricum NRRL 67062 to produce butyric acid during glucose fermentation was evaluated. The evaluated for substrate and product inhibition in batch experiments using anaerobic tubes. To characterize inhibition, initial concentrations ranging from 60 250 g L−1 were used, it demonstrated that concentration exerted strong on cell growth fermentation. evaluate culture challenged with 5–50 at an pH 6.5. These performed without control....
This study reports on a novel technique to enhance the high cell mass and viable counts of heterofermentative probiotic strain, Limosilactobacillus reuteri. is first report cultivation L. reuteri, which was incorporated with weak base anion-exchange resins remove accumulating lactic acid in fermentation broth. Two resins—Amberlite IRA 67 96—were found have adsorption capacity acid. Batch fed-batch were further analyzed using resins, as this application resulted higher maximum number cells....
ABSTRACT Extrusion method has been used to cook different food materials by employing the combination of high temperature, pressure and shearing stresses. Effects extrusion cooking on functional, physicochemical nutritional properties common bean (Phaseolus vulgaris L.) have reported for years. However, relatively few investigations done whole seed extrudates beans. Extruded products four beans including dark red kidney, black, pinto great northern were evaluated water absorption,...