- Bee Products Chemical Analysis
- Insect and Pesticide Research
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Insect-Plant Interactions and Control
Czech Agrifood Research Center
2018-2024
Czech University of Life Sciences Prague
2018-2020
Abstract Honeybee workers undergo metamorphosis in capped cells for approximately 13 days before adult emergence. During the same period, Varroa mites prick defenseless host many times. We sought to identify proteome differences between emerging -parasitized and parasite-free honeybees showing presence or absence of clinical signs deformed wing virus ( DWV ) cells. A label-free proteomic analysis utilizing nanoLC coupled with an Orbitrap Fusion Tribrid mass spectrometer provided a...
The ectoparasitic mite Varroa destructor transmits and triggers viral infections that have deleterious effects on honey bee colonies worldwide. We performed a manipulative experiment in which worker bees collected at emergence were exposed to for 72 h, their proteomes compared with those of untreated control bees. Label-free quantitative proteomics identified 77 differentially expressed A. mellifera proteins (DEPs). In addition, by orthogonal analysis, most importantly, Deformed wing virus...