A5028121069- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect symbiosis and bacterial influences
- Allergic Rhinitis and Sensitization
- Study of Mite Species
- Insect-Plant Interactions and Control
- Bee Products Chemical Analysis
- Insect Resistance and Genetics
- Insects and Parasite Interactions
- Food Allergy and Anaphylaxis Research
- Vector-borne infectious diseases
- Insect Pest Control Strategies
- Entomopathogenic Microorganisms in Pest Control
- Pesticide Residue Analysis and Safety
- Pesticide and Herbicide Environmental Studies
- Insect Utilization and Effects
- Weed Control and Herbicide Applications
- Entomological Studies and Ecology
- Environmental Toxicology and Ecotoxicology
- Bacterial Identification and Susceptibility Testing
- Studies on Chitinases and Chitosanases
- Healthcare and Venom Research
- Bird parasitology and diseases
- Advanced biosensing and bioanalysis techniques
Czech Agrifood Research Center
2015-2024
Medical Healthcom (Czechia)
2011
Charles University
2008
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed relative proportions P. within honeybee microbiome using metabarcoding analysis 16 S rRNA gene. The was analyzed in outside AFB zone (control - AFB0), from asymptomatic colonies an apiary (AFB1), and exhibiting clinical symptoms (AFB2). processed for entire community a cut-off comprising pathogenic/environmental bacteria...
Honey is a unique natural product produced by European honeybees. Due to its high economic value, honey considered be well characterized chemically, and it often discovered an adulterated commodity. However, this study shows that our knowledge of protein composition, which medical pharmaceutical importance, incomplete. In in-depth proteomic 13 honeys, we identified number proteins are important for understanding properties merit additional research. Our major result expanded the underlying...
Background Melissococcus plutonius is an entomopathogenic bacterium that causes European foulbrood (EFB), a honeybee ( Apis mellifera L.) disease necessitates quarantine in some countries. In Czechia, positive evidence of EFB was absent for almost 40 years, until outbreak the Krkonose Mountains National Park 2015. This occurrence gave us opportunity to study epizootiology by focusing on microbiome workers, which act as vectors diseases within and between colonies. Methods The included worker...
Tyrophagus putrescentiae (Schrank, 1781) is an emerging source of allergens in stored products and homes. Feces proteases are the major astigmatid mites (Acari: Acaridida). In addition, carriers microorganisms microbial adjuvant compounds that stimulate innate signaling pathways. We sought to analyze mite feces proteome, proteolytic activities, mite-bacterial interaction dry dog food (DDF). Proteomic methods comprising enzymatic zymographic analysis 2D-E-MS/MS were performed. The highest...
Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in populations. We compared bacterial communities of five populations T. one mixed population fanetzhangorum collected from habitats. Material: six mite diets were Sanger sequencing cloned 16S rRNA obtained amplification with universal eubacterial primers using taxon-specific samples...
Abstract We investigated pathogens in the parasitic honeybee mite Varroa destructor using nanoLC-MS/MS (TripleTOF) and 2D-E-MS/MS proteomics approaches supplemented with affinity-chromatography to concentrate trace target proteins. Peptides were detected from currently uncharacterized Macula-like virus (VdMLV), deformed wing (DWV)-complex acute bee paralysis (ABPV). Peptide alignments revealed detection of complete structural DWV-complex block VP2-VP1-VP3, VDV-1 helicase single-amino-acid...
The winter beehive debris containing bodies of honeybee parasitic mite Varroa destructor is used for veterinary diagnostics. sucking haemolymph serves as a reservoir pathogens including bacteria. Worker bees can pick up from the during cleaning activities and spread infection to healthy within colony. aim this study was detect entomopathogenic bacteria in collected debris. Culture‐independent approach analyse mite‐associated bacterial community. Total DNA extracted samples 10 female...
Abstract BACKGROUND Imidacloprid‐urea is the primary imidacloprid soil metabolite, whereas imidacloprid‐olefin main plant‐relevant metabolite and more toxic to insects than imidacloprid. We artificially contaminated potting used quantitative UHPLC‐QqQ‐MS / MS determine imidacloprid, imidacloprid‐urea distributions in rapeseed green plant tissues roots after 4 weeks of exposure. RESULTS In soil, imidacloprid/imidacloprid‐urea molar ratios decreased similarly 250 2500 µg kg −1 treatments. The...
The pH of the guts mites strongly affects their digestive processes. This study was carried out to determine in 12 species stored product and house dust mites. Eighteen indicators were chosen offered feeding biotest. Based on color changes indicators, gut contents acaridid determined be within a range 4 neutral. showed gradient from anterior posterior part. midgut (ventriculus caeca) most had ranging 4.5 5, or slightly more alkaline for species, while middle (intercolon/colon) 5 6. Finally,...
House dust mites (HDMs) and stored-product (SPMs) of various species inhabit human homes stored agricultural products. These are carriers hosts microorganisms that enable their survival. The bacteriome from 13 SPMs HDMs was analyzed compared by 454 pyrosequencing partial 16S rRNA gene amplicons. Altogether 128,052 sequences were obtained assigned to 71 operational taxonomic units (OTUs) at the 97% identity level. number in OTUs between ranged 6 31 individual mite species. We did not find any...
Abstract Honeybee workers undergo metamorphosis in capped cells for approximately 13 days before adult emergence. During the same period, Varroa mites prick defenseless host many times. We sought to identify proteome differences between emerging -parasitized and parasite-free honeybees showing presence or absence of clinical signs deformed wing virus ( DWV ) cells. A label-free proteomic analysis utilizing nanoLC coupled with an Orbitrap Fusion Tribrid mass spectrometer provided a...
Domestic gardens supply pollinators with valuable habitats, but the risk of exposure to pesticides has been little investigated. Artificial nesting shelters a red mason bee species (Osmia bicornis) were placed in two suburban and commercial fruit orchards determine contamination forage sources by pesticides. Larval pollen provisions collected from total 14 nests. They consisted mainly oaks (65–100% weight/sample), Brassicaceae (≤34% w/s) trees (≤1.6% w/s). Overall, 30 detected each sample...