A5021636253- Anesthesia and Neurotoxicity Research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Cardiac Ischemia and Reperfusion
- Intensive Care Unit Cognitive Disorders
- Anesthesia and Sedative Agents
- Electrospun Nanofibers in Biomedical Applications
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Fungal and yeast genetics research
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Biofuel production and bioconversion
- Cancer Cells and Metastasis
- Cardiac Fibrosis and Remodeling
- Wound Healing and Treatments
- RNA Research and Splicing
- Cancer Research and Treatments
- RNA regulation and disease
- Congenital heart defects research
- Pancreatic function and diabetes
- ATP Synthase and ATPases Research
Medical College of Wisconsin
2015-2024
Tianjin Medical University
2024
Tianjin Medical University Eye Hospital
2024
The University of Texas MD Anderson Cancer Center
2006-2021
Tulane University
2009-2021
Peking University
2004-2020
Tianjin University of Science and Technology
2014-2018
Stanford University
2017
Novem (Netherlands)
2015
American Society of Anesthesiologists
2014-2015
This study was designed to assess whether intracoronary application of adipose tissue-derived stem cells (ADSCs) compared with bone marrow-derived (BMSCs) and control could improve cardiac function after 30 days in a porcine acute myocardial infarction/reperfusion model.An transmural infarction induced by inflating an angioplasty balloon for 180 min the mid-left anterior descending artery. Two million cultured autologous were injected through central lumen inflated catheter. Analysis...
Soft tissue loss presents an ongoing challenge in reconstructive surgery. Local stem cell application has recently been suggested as a possible novel therapy. In the present study we evaluated potential of silk fibroin-chitosan (SFCS) scaffold serving delivery vehicle for human adipose-derived cells (ASCs) murine soft injury model. Green fluorescent protein (GFP)-labeled ASCs were seeded on SFCS scaffolds at density 1 x 10(5) per cm(2) 48 hours and then suture-inlaid to 6-mm, full-thickness...
We assessed whether freshly isolated human adipose tissue-derived cells (fhADCs) or cultured stem (hASCs) have beneficial effects on cardiac function after myocardial infarction (MI), the injected can survive long term, and their result from direct differentiation paracrine mechanisms.Myocardial was experimentally induced in severe combined immunodeficient mice, either fhADCs, hASCs, phosphate-buffered saline into peri-infarct region. Myocardial improved significantly mice treated with hASCs...
Although MSCs (mesenchymal stem cells) and fibroblasts have been well studied, differences between these two cell types are not fully understood. We therefore comparatively analysed antigen gene profiles, colony-forming ability differentiation potential of four human in vitro: commercially available skin-derived [hSDFs (human fibroblasts)], adipose tissue-derived cells [hASCs cells)], embryonic lung (WI38) dermal microvascular endothelial [hECs cells)].hSDFs, hASCs WI38 exhibited a similar...
Growing evidence indicates that ketamine causes neurotoxicity in a variety of developing animal models, leading to serious concern regarding the safety pediatric anesthesia. However, if and how induces human neural cell toxicity is unknown. Recapitulation neurogenesis from embryonic stem cells (hESCs) vitro allows investigation toxic effects on (NSCs) neurons, which impossible perform humans. In present study, we assessed influence hESC-derived NSCs neurons.hESCs were directly differentiated...
Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs) (iPSC-CMs) are a promising source for myocardial regeneration, disease modeling and drug assessment. However, iPSC-CMs exhibit immature fetal CM-like characteristics that different from adult CMs in several aspects, including cellular structure metabolism. As an example, glycolysis is major energy CMs. mature, the mitochondrial oxidative capacity increases, with fatty acid β-oxidation becoming key to meet heart’s high...
Recent studies in various animal models have suggested that anesthetics such as propofol, when administered early life, can lead to neurotoxicity. These raised significant safety concerns regarding the use of pediatric population and highlight need for a better model study anesthetic-induced neurotoxicity humans. Human embryonic stem cells are capable differentiating into any cell type represent promising mechanisms governing neurotoxicity.Cell death human cell-derived neurons was assessed...
Ketamine is widely used for anesthesia in pediatric patients. Growing evidence indicates that ketamine causes neurotoxicity a variety of developing animal models. Our understanding humans currently limited by difficulties obtaining neurons and performing developmental toxicity studies fetal populations. It may be possible to overcome these challenges from human embryonic stem cells (hESCs) vitro. hESCs are able replicate indefinitely differentiate into every cell type. In this study, we...
Abstract Maternal alcohol exposure during pregnancy can substantially impact the development of fetus, causing a range symptoms, known as fetal spectrum disorders (FASDs), such cognitive dysfunction and psychiatric disorders, with pathophysiology mechanisms largely unknown. Recently developed human cerebral organoids from induced pluripotent stem cells are similar to brains in aspects structure. These models allow more relevant vitro systems be for studying FASDs than animal models. Modeling...
Human adipose tissue-derived stem cells (hASCs) represent a potentially valuable cell source for clinical therapeutic applications. The present study was designed to investigate properties of ionic channel currents in undifferentiated hASCs and their impact on proliferation. functional ion channels were analyzed by whole-cell patch-clamp recording mRNA expression levels detected RT-PCR. Four types found be hASCs: most the (73%) showed delayed rectifier-like K(+) current (I(KDR));...
Abstract Background Studies in developing animals have shown that anesthetic agents can lead to neuronal cell death and learning disabilities when administered early life. Development of human embryonic stem cell–derived neurons has provided a valuable tool for understanding the effects anesthetics on neurons. Unbalanced mitochondrial fusion fission various pathological conditions including neurodegeneration. The aim this study was dissect role dynamics propofol-induced neurotoxicity....
Growing animal evidence demonstrates that prolonged exposure to propofol during brain development induces widespread neuronal cell death, but there is little information on the role of astrocytes. Astrocytes can release neurotrophic growth factors such as brain-derived factor (BDNF), which exert protective effect neurons in paracrine fashion. We hypothesize anesthesia, BDNF released from developing astrocytes may not be sufficient prevent propofol-induced neurotoxicity.Hippocampal and...
Background: The development of 3D cerebral organoid technology using human-induced pluripotent stem cells (iPSCs) provides a promising platform to study how brain diseases are appropriately modeled and treated. So far, understanding the characteristics organoids is still in its infancy. current profiled, for first time, electrophysiological properties at molecular cellular levels dissected potential age equivalency 2-month-old human ones by comparison gene expression profiles among...
JOURNAL/rmrep/04.03/02273995-202506000-00001/figure1/v/2025-04-06T105240Z/r/image-tiff Heart failure is a pressing clinical condition that expected to increase as our population ages and thus requires better treatment options. Identifying the precise mechanisms underlie fibrosis inflammation, two key features associated with cardiac repair regeneration during ischemic nonischemic heart failure, likely provide effective strategies for intervention of failure. This study investigated metabolic...