A5064432607- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- Peroxisome Proliferator-Activated Receptors
- Retinoids in leukemia and cellular processes
- Connexins and lens biology
- Diet and metabolism studies
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic Associations and Epidemiology
- Biochemical effects in animals
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive System and Pregnancy
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Estrogen and related hormone effects
- Circular RNAs in diseases
- Nuclear Receptors and Signaling
- Pharmacology and Obesity Treatment
- Redox biology and oxidative stress
- NF-κB Signaling Pathways
- Pharmacogenetics and Drug Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Birth, Development, and Health
Charles University
2009-2022
General University Hospital in Prague
2017-2022
The spontaneously hypertensive rat (SHR) is the most widely used model of essential hypertension and susceptible to left ventricular hypertrophy (LVH) myocardial fibrosis. Recently, a quantitative trait locus (QTL) that influences heart interstitial fibrosis was mapped chromosome 8. Our aim dissect genetic basis this QTL(s) predisposing SHR hypertension, LVH, Hemodynamic histomorphometric analyses were performed in genetically defined SHR.PD-chr.8 minimal congenic strain (PD5 subline) rats....
Therapeutic administration of retinoids is often accompanied with undesirable side effects, including an increase in lipid levels up to 45% treated patients. We tested the hypothesis whether spontaneously hypertensive rat (SHR) and congenic SHR.PD-(D8Rat42-D8Arb23)/Cub (SHR-Lx) strains, differing only a 14-gene region chromosome 8 previously shown display differential sensitivity teratogenic effects retinoic acid, could serve as pharmacogenetic model set metabolic retinoid therapy.Male,...
We assessed the effect of previously uncovered gap junction protein alpha 8 (Gja8) mutation present in spontaneously hypertensive rat – dominant cataract (SHR-Dca) strain on blood pressure, metabolic profile, and heart renal transcriptomes. Adult, standard chow-fed male rats SHR SHR-Dca strains were used. found a significant, consistent 10-15 mmHg decrease both systolic diastolic pressures compared with (P<0.01 P<0.05, respectively; repeated measures analysis variance (ANOVA)). With...
Background: Glucocorticoids are potent therapeutic agents frequently used for treatment of number conditions, including hematologic, inflammatory and allergic diseases. Both their adverse effects display significant interindividual variation, partially attributable to genetic factors. We have previously isolated a seven-gene region rat chromosome 8 sensitizing dexamethasone (DEX)-induced dyslipidemia insulin resistance skeletal muscle. Using two newly derived congenic strains we aimed...
All-trans retinoic acid (ATRA, tretinoin) is a vitamin A derivative commonly used in the treatment of diverse conditions ranging from cancer to acne. In fraction predisposed individuals, administration ATRA accompanied by variety adverse metabolic effects, particularly induction hyperlipidemia. We have previously derived minimal congenic SHR.PD-(D8Rat42-D8Arb23)/Cub (SHR-Lx) strain sensitive ATRA-induced increase triacylglycerols and cholesterol under condition high-sucrose diet. SHR-Lx...
Abstract Dexamethasone (DEX) is known to induce diabetes and dyslipidemia. We have compared fasting triacylglycerol cholesterol concentrations across 20 lipoprotein fractions glucose tolerance in control (standard diet) DEX-treated 7-month-old males of two rat strains, Brown Norway (BN) congenic BN.SHR-( Il6 - Cd36 )/Cub (BN.SHR4). These inbred strains differ a defined segment chromosome 4, originally transferred from the spontaneously hypertensive (SHR) including mutant gene, target DEX....
The plasma profile of major lipoprotein classes and its subdivision into particular fractions plays a crucial role in the pathogenesis atherosclerosis is predictor coronary artery disease. Our aim was to identify genomic determinants triglyceride cholesterol distribution particle sizes recombinant inbred rat set PXO, which alleles two models metabolic syndrome (SHR PD strains) segregate together with those from Brown Norway strain. Adult male rats 15 PXO strains (n = 8–13/strain) progenitor...
Metabolic syndrome is a highly prevalent human disease with substantial genomic and environmental components. Previous studies indicate the presence of significant genetic determinants several features metabolic on rat chromosome 16 (RNO16) syntenic regions genome. We derived SHR.BN16 congenic strain by introgression limited RNO16 region from Brown Norway (BN-Lx) into background spontaneously hypertensive (SHR) strain. compared morphometric, metabolic, hemodynamic profiles adult male SHR...
Several members of connexin family transmembrane proteins were previously implicated in distinct metabolic conditions. In this study we aimed to determine the effects complete and heterozygous form connexin50 gene (Gja8) mutation L7Q on profile oxidative stress parameters spontaneously hypertensive inbred rat strain (SHR). Adult, standard chow-fed male rats SHR, SHR-Dca+/− SHR-Dca−/− coisogenic strains used. At age 4 months, dexamethasone (2.6 μg/ml) was administered drinking water for three...
Several corresponding regions of human and mammalian genomes have been shown to affect sensitivity the manifestation metabolic syndrome via nutrigenetic interactions. In this study, we assessed effect sucrose administration in a newly established congenic strain BN.SHR20, which limited segment rat chromosome 20 from model, spontaneously hypertensive (SHR), was introgressed into Brown Norway (BN) genomic background. We mapped extent differential compared sequences BN vs. SHR within silico....
Background: Polydactylous rat strain PD/Cub and spontaneously hypertensive SHR are two established rodent models of human metabolic syndrome. In the process positional cloning apparently pleiotropic locus on chromosome 8 affecting major features syndrome including dyslipidemia we have derived minimal congenic SHR.PD-(D8Rat42-D8Arb23)/Cub (SHR-Lx) carrying only 7 genes PD origin background. Design: Adult male rats SHR-Lx strains were fed standard diet (STD) subsequently treated with retinoic...
Metabolic syndrome is a frequent condition with multifactorial aetiology. Previous studies indicated the presence of genetic determinants metabolic components on rat chromosome 2 (RNO2) and syntenic regions human genome. Our aim was to further explore these findings using novel models. We derived BN-Dca BN-Lx.Dca congenic strains by introgression limited RNO2 region from spontaneously hypertensive strain carrying mutation in Gja8 gene (SHR-Dca, dominant cataract) into genomic background...