A5055338438- Fluorine in Organic Chemistry
- Chemical Reaction Mechanisms
- Chemical Synthesis and Reactions
- Diet, Metabolism, and Disease
- Synthesis and Biological Evaluation
- Radical Photochemical Reactions
- Inorganic Fluorides and Related Compounds
- Electrochemical Analysis and Applications
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Birth, Development, and Health
- Synthesis and Reactions of Organic Compounds
- Oxidative Organic Chemistry Reactions
- Epigenetics and DNA Methylation
- Retinoids in leukemia and cellular processes
- Chemical Synthesis and Analysis
- Inorganic and Organometallic Chemistry
- Congenital limb and hand anomalies
- Synthesis and Characterization of Heterocyclic Compounds
- Sulfur-Based Synthesis Techniques
- Pancreatic function and diabetes
- Organic Chemistry Cycloaddition Reactions
- Wnt/β-catenin signaling in development and cancer
- Synthesis and Reactivity of Heterocycles
- Phenothiazines and Benzothiazines Synthesis and Activities
Charles University
2016-2025
General University Hospital in Prague
2017-2024
Czech Academy of Sciences
2017-2023
Czech Academy of Sciences, Institute of Physiology
2017-2023
Czech Academy of Sciences, Institute of Molecular Genetics
2017
Sapienza University of Rome
2017
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2012
Max Delbrück Center
2009
University of Chemistry and Technology, Prague
1994-2005
Ricardo Prague (Czechia)
1995-2000
The hypodactylous (hd) locus impairs limb development and spermatogenesis, leading to male infertility in rats. We show that the hd mutation is caused by an insertion of endogenous retrovirus into intron 10 Cntrob gene. retroviral mutant rats disrupts normal splicing transcripts results expression a truncated protein. During final phase spermiogenesis, centrobin localizes manchette, centrosome, marginal ring spermatid acroplaxome, where it interacts with keratin 5-containing intermediate...
Autosomal dominant craniometaphyseal dysplasia (AD-CMD) is a rare condition defined by the occurrence of progressive diffuse hyperostosis cranial bones and abnormal metaphyseal widening tubular bones. ANKH known to be only gene associated with AD-CMD. We present case toddler boy macrodolichocephaly, asymmetry skull, wide bulging forehead, gingival hypertrophy irregular teeth. Physical examination, X-ray DNA analysis were performed. All exons flanking intron regions amplified PCR directly...
The spontaneously hypertensive rat (SHR), one of the most widely used model essential hypertension, is predisposed to left ventricular hypertrophy, myocardial fibrosis, and metabolic disturbances. Recently, quantitative trait loci influencing blood pressure, mass, heart interstitial fibrosis were genetically isolated within a minimal congenic subline that contains only 7 genes, including mutant Plzf (promyelocytic leukemia zinc finger) candidate gene. To identify as gene, we targeted in SHR...
A series of 2-pyridineketoximes with paraffinic chains different lengths (CH 3 , 1a; C 8 H 17 1b; 13 27 1c) has been synthesized, and their complexes Cu(II), Co(II), Zn(II), Ni(II) have investigated in the cleavage p-nitrophenyl esters carboxylic phosphoric acids water (1a) or comicelles CTABr (1b,c). While Co(II) Cu(II) are ineffective promoting acetate (PNPA) hexanoate (PNPH) esters, Zn(II) especially strongly accelerate such substrates. With latter metal ion, effective species is a...
The spontaneously hypertensive rat (SHR) is the most widely used model of essential hypertension and susceptible to left ventricular hypertrophy (LVH) myocardial fibrosis. Recently, a quantitative trait locus (QTL) that influences heart interstitial fibrosis was mapped chromosome 8. Our aim dissect genetic basis this QTL(s) predisposing SHR hypertension, LVH, Hemodynamic histomorphometric analyses were performed in genetically defined SHR.PD-chr.8 minimal congenic strain (PD5 subline) rats....
The polydactylous rat strain (PD/Cub) is a highly inbred (F > 90) genetic model of metabolic syndrome. aim this study was to analyze the architecture derangements found in PD/Cub and assess its dynamics time response diet medication. We derived x BN/Cub (Brown Norway) F2 intercross population 149 male rats performed profiling genotyping multiple levels linkage statistical analyses at five different stages ontogenesis after high-sucrose feeding dexamethasone administration challenges....
Abstract Lx mutation in SHR. rat manifests homozygotes as hindlimb preaxial polydactyly. It was previously mapped to a chromosome 8 segment containing the Plzf gene. (promyelocytic leukemia zinc finger protein) influences limb development direct repressor of posterior HoxD genes. However, coding sequence is intact mutants. Using linkage mapping F2 hybrids, we downsized 155 kb and sequenced conserved noncoding elements (CNEs) inside. A 2,964‐bp deletion intron 2, never detected control...
The SHR and the PD/Cub are two established rodent models of human metabolic syndrome. Introgression a ca 30 cM region rat chromosome 8 from onto genetic background was previously shown to influence several syndrome-related traits along with causing PLS in SHR- Lx congenic strain. In process identification causative alleles, we have produced sublines. differential segment PD5 substrain [SHR.PD( D8Rat42-D8Arb23 )/Cub] spans approximately 1.4 Mb encompassing only 14 genes. When comparing...
Therapeutic administration of retinoids is often accompanied with undesirable side effects, including an increase in lipid levels up to 45% treated patients. We tested the hypothesis whether spontaneously hypertensive rat (SHR) and congenic SHR.PD-(D8Rat42-D8Arb23)/Cub (SHR-Lx) strains, differing only a 14-gene region chromosome 8 previously shown display differential sensitivity teratogenic effects retinoic acid, could serve as pharmacogenetic model set metabolic retinoid therapy.Male,...
2,2-Dinitroethene-1,1-diamine (FOX-7) is a compound synthesized in 1998 with simple structure but very unusual properties. One part of the molecule bears two geminal electron-withdrawing nitro groups as strong oxidation center and opposite represented by electron-donating amino groups. This combination creates so called push-pull effect electrons. The electron distribution unbalanced enabling intramolecular transfer reactions, what typical for energetic material. Many studies appeared during...
Recently, it has been found that spontaneous mutation Lx (polydactyly-luxate syndrome) in the rat is determined by deletion of a conserved intronic sequence Plzf (Promyelocytic leukemia zinc finger protein) gene. In addition, prominent candidate gene for quantitative trait loci (QTLs) associated with cardiac hypertrophy and fibrosis spontaneously hypertensive (SHR). current study, we tested effects targeting SHR using TALENs (transcription activator-like effector nucleases). ova were...
Three potential reactivators of nerve agents-inhibited acetylcholinesterase: 2-[(hydroxyimino)phenylmethyl]-1-methylpyridinium iodide 3a, 2-[(hydroxyimino)pyridin-2-ylmethyl]-1-methylpyridinium 3b and 2-[(1-hydroxyimino) ethyl]-1-methylpyridinium 3c were synthesized. Their reactivation potency was examined using a standard in vitro test. A rat brain homogenate used as the source acetylcholinesterase. compared with currently acetylcholinesterase reactivator - 2-PAM (pralidoxime) 4. All tested...
Reactivators of acetylcholinesterase (AChE; EC 3.1.1.7) are able to treat intoxication by organophosphorus compounds, especially with pesticides or nerve agents. Owing the fact that there exists no universal "broad-spectrum" reactivator organophosphates-inhibited AChE, many laboratories have synthesized new AChE reactivators. Here, we five and three previously known quaternary monopyridinium oximes as potential reactivators inhibited Potencies cleave p-nitrophenyl acetate (PNPA), which is...
Endogenous retroviruses (ERVs) contribute to a range of germline, as well somatic mutations in mammals. However, autonomous retrotransposition potentially active elements has not been demonstrated the rat genome. We cloned an insertion that disrupted normal splicing Cntrob gene was subsequently identified nonautonomous, novel endogenous retrovirus RnERV-K8e family. The family is closely related recently reported MmERV-K10c elements, but differs from mouse MusD or IAP families. In addition,...
Metribuzin [4-amino-6-tert-butyl-3-methylthio-1,2,4-triazin-5(4H)-one] is a widely used weed controlling agent. In aqueous solutions and in 30 % v/v acetonitrile/water it reduced two two-electron steps. both steps protonated forms of azomethine bonds are reduced, the more positive 1,6-bond, negative (by about 0.4 V) 2,3-bond. At pH > 7 conjugated base at considerably potentials one-electron step. Reduction accompanied by acid-base hydration-dehydration equilibria. The 1,6-dihydrometribuzin,...
A series of quaternized alkyl pyridyl ketoximes was synthesized and tested as micellar hydrolytic catalysts. 2- And 4-[1-(hydroxyimino)tridecyl]-1-methylpyridinium bromides were surprisingly efficient, most probably due to the location their nucleophilic hydroxyimino group below surface. Absorbance reaction mixture vs time plots exhibited remarkable positive deviation from first-order kinetics when hydrolysis 4-nitrophenyl phosphates catalyzed by 1-dodecyl-3-[1-(hydroxyimino)ethyl]- or...
Ten meta - and para -substituted benzoic acids with substituents N + (CH 3 ) , CH 2 Py SO PO(OCH were synthesized. Dissociation constants of these determined in five solvents (water, ethanol, methanol, -dimethylformamide, dimethyl sulfoxide) at 25 °C. acid derivatives other H, NHCOCH OCH F, Cl, Br, I, COCH CN, NO taken from the literature (calibration set). Substituent σ m p I R i for calculated by nonlinear PLS (partial least-square method latent variables) calibration three correlation...