A5026498854- Reproductive Biology and Fertility
- Effects and risks of endocrine disrupting chemicals
- Pleistocene-Era Hominins and Archaeology
- Archaeology and ancient environmental studies
- Silicon Effects in Agriculture
- Geology and Paleoclimatology Research
- Education, Psychology, and Social Research
- Phytoestrogen effects and research
- Signaling Pathways in Disease
- Microplastics and Plastic Pollution
- Sperm and Testicular Function
- Birth, Development, and Health
- Seed Germination and Physiology
- Ecology and Vegetation Dynamics Studies
- Plant and animal studies
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Sulfur Compounds in Biology
- Sirtuins and Resveratrol in Medicine
- Toxic Organic Pollutants Impact
- Mycotoxins in Agriculture and Food
- Botanical Studies and Applications
- Histone Deacetylase Inhibitors Research
- Macrophage Migration Inhibitory Factor
- Rangeland and Wildlife Management
Charles University
2020-2024
Czech Academy of Sciences, Nuclear Physics Institute
2023
Institute of Animal Science
2018-2023
Czech Academy of Sciences, Institute of Botany
2021
Czech University of Life Sciences Prague
2013-2017
Bisphenol A (BPA), a chemical component of plastics, is widely distributed environmental pollutant and contaminant water, air, food that negatively impacts human health. Concerns regarding BPA have led to the use BPA-free alternatives, one which bisphenol S (BPS). However, effects BPS are not well characterized, its specific on reproduction fertility remain unknown. It therefore necessary evaluate any mammalian oocytes. The present study first demonstrate markedly negative pig oocyte...
Bisphenols belong to the endocrine disruptors, affecting reproduction even in extremely low doses. Bisphenol S (BPS) has become widely used as a substitute for earlier-used bisphenol A; however, its harmlessness is questionable. The aim of this study was evaluate effect BPS on folliculogenesis and oocyte quality after vivo exposure doses BPS. Four-week-old ICR females ( n = 16 each experimental group) were exposed vehicle control (VC), BPS1 (0.001 ng BPS.g/bw/day), BPS2 (0.1 ng.g/bw/day),...
Bisphenol S (BPS), the main replacement for bisphenol A (BPA), is thought to be toxic, but limited information available on effects of ovarian follicles. In our study, we demonstrated presence in follicular fluid women at a concentration 22.4 nM. The effect such concentrations oocyte maturation and subsequent embryo development still unknown. Therefore, focused vitro maturation, fertilization, development. As model, used porcine oocytes, which show many physiological similarities human...
The histone code is an established epigenetic regulator of early embryonic development in mammals. lysine residue K9 H3 (H3K9) a prime target SIRT1, member NAD+-dependent deacetylase family enzymes targeting both and non-histone substrates. At present, little known about SIRT1-modulation H3K9 zygotic pronuclei its association with the success preimplantation embryo development. Therefore, we evaluated effect SIRT1 activity on methylation acetylation porcine zygotes significance modifications...
Abstract Background Archaeobotanists and palaeoecologists use geometric morphometrics extensively to identify plant opal phytoliths. Particularly when applied assemblages of phytoliths from concentrations retrieved closed contexts, morphometric data archaeological compared with similar reference material can allow taxonomic attribution. Observer variation is one aspect phytolith morphometry that has received little attention but might be an important source error a potential cause...
SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that involved in oocyte maturation; therefore, the importance of ooplasmic pool for further fate mature oocytes has been strongly suggested. We hypothesised plays role a signalling molecule through selected regulation. observed re-localisation its association with spindle microtubules. In oocytes, distribution shows spindle-like pattern, spindle-specific action decreases α-tubulin acetylation. Based...
The relative contributions of inter- and intraspecific variation to phytolith shape size have only been investigated in a limited number studies. However, detailed understanding patterns among populations or even within single plant specimen is key importance for the correct taxonomic identification grass taxa fossil samples reconstruction vegetation environmental conditions past. In this study, we used geometric morphometric analysis quantification different sources variation.We...
Alpine grassland vegetation supports globally important biodiversity and ecosystems that are increasingly threatened by climate warming other environmental changes. Trait-based approaches can support understanding of responses to global change drivers consequences for ecosystem functioning. In six sites along a 1314 m elevational gradient in Puna grasslands the Peruvian Andes, we collected datasets on vascular plant composition, functional traits, biomass, fluxes, data over three years. The...
Calcineurin is required for oocyte exit from meiotic block in metaphase II (MII) stage invertebrates and also lower vertebrates. However, the role of calcineurin mammalian activation still unclear. The aim this study was to determine whether involved processes regulating porcine activation. Indirect immunofluorescence demonstrated localization both subunits, CnA CnB, especially cortex area MII oocytes, vitro fertilized parthenogenetically activated oocytes. After activation, fluorescence...
Abstract Perennial herbaceous species form their above‐ground parts every year anew and discard them before the advent of winter. The senescence structures is thus an inevitable part life cycle. This also a key process that determines photosynthetic gain late in season economy soil‐borne nutrients. Here we address patterns drivers shoot perennial plants. We present comparative study 231 temperate species, ranging from spring ephemeroids to senescing autumn, common botanical garden...
Using pollen analysis, phytolith analysis and radiocarbon dating of newly discovered sedimentary archives, here we prove the native status European larch (Larix decidua Mill.) in two sandstone rocky areas north Bohemia (Czech Republic). At end Last Ice Age at beginning Holocene, was relatively common Central Europe. In most critical period Middle it demonstrably retreated to exposed habitats, but did not become completely extinct. form relict populations, survived locally until modern era,...
The evolutionary success of termites has been driven largely by a complex communication system operated rich set exocrine glands. As many as 20 different organs are known in termites. While some these relatively well known, only anecdotal observations exist for others. One the that received negligible attention so far is labral gland. In this study, we examined structure and ultrastructure labrum soldiers 28 termite species. We confirm gland present all species, comprises two secretory...
The objective of the present study was to examine if luteal blood flow (LBF) monitoring could be used as an additional prognostic tool for early pregnancy diagnosis, and we particularly focused on differences in LBF between pregnant nonpregnant mares. Furthermore, other possible developmental corpus luteum (CL) mares were evaluated. CL (n = 119) 27 monitored once daily B- Power-Doppler Mode days 1, 2, 9, 12, 16 after ovulation (day 0 ovulation). data evaluated using MIXED Linear Model with...
Hydrogen sulfide has been shown to improve the quality of oocytes destined for in vitro fertilization. Although hydrogen is capable modulating ion channel activity somatic cells, role gametes and embryos remains unknown. Our observations confirmed hypothesis that KATP L-type Ca2+ channels play roles porcine oocyte ageing revealed a plausible contribution modulation activity. We benefits activation suppression channels, respectively, preservation quality. experiments identified as promoting...
In the sphere of reproductive biotechnologies, demand for sufficient numbers high-quality oocytes is still increasing. some cases, this obstacle overcome by in vitro prolonged cultivation. However, a oocyte culture accompanied changes called ageing. Ageing manifested spontaneous parthenogenetic activation, programmed cell death or lysis. Various substances, such as caffeine dithiothreitol, have been tested ageing suppression. respect, research into gasotransmitters (hydrogen sulphide, carbon...
Abstract Background and Aims Grass silica short cell (GSSC) phytoliths appear to be the most reliable source of fossil evidence for tracking evolutionary history paleoecology grasses. In recent years, modern techniques that quantitatively assess phytolith shape variation have widened opportunities classification grass phytoliths. However, phylogenetic, ecological intraindividual variability patterns in remain largely unexplored. Methods The full range [3650 two-dimensional (2-D) outlines]...
The cumulus expansion of cumulus-oocyte complex (COC) is an essential regulating process oocyte maturation and as such it a possible biomarker the in vitro maturing oocytes quality. Cumulus usually assessed by non-invasive methods based on visual evaluation with many inaccuracies. On other hand, analytical measurement quantity hyaluronic acid (HA), most abundant compound expanded cumuli, one to evaluate precisely. Therefore, this study aimed verify applicability HA analysis for evaluating...
Radiouhlíkové datování je etablovanou metodou, která pomáhá určit absolutní stáří archeologických nálezů. Tento tematický přehled prezentuje základní principy radiouhlíkové metody, předpoklady pro výběr vzorků z situací, způsob zacházení se vzorky před zasláním do laboratoře, laboratorní postupy úpravy vzorků, průběh AMS měření a kalibraci výsledků. Dále jsou vysvětleny faktory, které omezují výsledky radiouhlíkového datování, zejména radiouhlíková plata rezervoárový efekt, jak lze jejich...
The authors present preliminary results from a new research project based in Jebel Shaqadud, Sudan. Their findings highlight the potential for this region's archaeological record to expand our understanding of adaptation strategies used by human groups arid north-east African environments away rivers and lakes during Holocene. Furthermore, they exceptionally early radiocarbon dates that push postglacial occupation eastern Sahel back twelfth millennium BP.