A5058842471- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Effects and risks of endocrine disrupting chemicals
- Ovarian function and disorders
- Toxic Organic Pollutants Impact
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Heat shock proteins research
- Diabetes and associated disorders
- Carcinogens and Genotoxicity Assessment
- Renal and related cancers
- Microplastics and Plastic Pollution
- Metabolism and Genetic Disorders
- Ubiquitin and proteasome pathways
- Hormonal and reproductive studies
- Bee Products Chemical Analysis
- Cancer, Lipids, and Metabolism
- Estrogen and related hormone effects
- Advanced Proteomics Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Mycotoxins in Agriculture and Food
- Infant Nutrition and Health
- Selenium in Biological Systems
- Cancer, Hypoxia, and Metabolism
- Drug Transport and Resistance Mechanisms
Czech Academy of Sciences, Institute of Biotechnology
2014-2025
Yale University
2020-2021
Czech Academy of Sciences
2009-2015
Charles University
2012
In mammals, germ cell differentiation is initiated in the Primordial Germ Cells (PGCs) during fetal development. Prenatal exposure to environmental toxicants such as endocrine disruptors may alter PGC differentiation, development of male germline and induce transgenerational epigenetic disorders. The anti-androgenic compound vinclozolin represents a paradigmatic example molecule causing effects on cells. We performed prenatal mice analyzed phenotypic molecular changes three successive...
Out of millions ejaculated sperm, a few reach the fertilization site in mammals. Flagellar Ca 2+ signaling nanodomains, organized by multi-subunit CatSper calcium channel complexes, are pivotal for sperm migration female tract, implicating CatSper-dependent mechanisms selection. Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on phosphorylation cascades. correlates with...
Abstract In patients with testicular germ cell tumours (TGCT), sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated low recovery rate after thawing. Since a high-energy demanding cell, which supplied by glycolysis and oxidative phosphorylation (OXPHOS), mitochondrial dysfunctionality can directly result in anomalies. this study, we investigated bioenergetic pattern of cryopreserved TGCT comparison normozoospermic...
Abstract Background Mammalian sperm must undergo a series of controlled molecular processes in the female reproductive tract called capacitation before they are capable penetrating and fertilizing egg. Capacitation, as complex biological process, is influenced by many factors, among which steroidal hormone estrogens play their role. Estrogens, present high concentration generally considered primarily hormones. However, there increasing evidence important impact on male parameters. The...
Toxoplasma gondii is a common protozoan parasite that infects warm-blooded animals throughout the world, including mice and humans. During infection, both, host, utilize various mechanisms to maximize their own reproductive success. Mice humans are both intermediate hosts for gondii, which forms specialized vacuoles containing cysts in formers' tissue. As half of human population infected, developing disease called toxoplasmosis, along with an ever-growing number couples suffering idiopathic...
Gamete fusion is a critical event of mammalian fertilization. A random one-bead one-compound combinatorial peptide library represented synthetic human egg mimics and identified previously unidentified ligand as Fc receptor–like 3, named MAIA after the mythological goddess intertwined with JUNO. This immunoglobulin super family receptor was expressed on oolemma played major role during sperm-egg adhesion fusion. forms highly stable interaction known IZUMO1/JUNO complex, permitting specific...
Tetrabromobisphenol A (TBBPA) is the main flame retardant used in printed circuit boards and laminates. The human population highly exposed to TBBPA as it consumer electronics well office communication equipment. use of hexabromocyclododecane (HBCD) insulation foam boards, which are widely construction sector. Brominated retardants may possess endocrine disrupting activity thus represent a threat environment, including humans their reproduction. aim this work was evaluate oestrogenic effects...
Summary Tetrabromobisphenol A ( TBBPA ) is a widely used brominated flame retardant of increasing concern to human health because its action as an endocrine disruptor. We have previously demonstrated that able increase apoptosis testicular cells and other changes in the first second generations mice exposed . However, potential effects on mouse epididymal spermatozoa not yet been investigated. Therefore, we initiated this study determine whether exposure could also result increased DNA...
The acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. It widely recognized that specific proteins form an active network prior fertilization, their dynamic relocation crucial for sperm-egg fusion. unique presence cofactor protein CD46 in acrosomal was shown, however, its behaviour connection with other has not been explored further. Using super resolution microscopy, we demonstrated reorganisation over head...
In order for mammalian sperm to obtain a fertilizing ability, they must undergo complex of molecular changes, called capacitation. During capacitation, steroidal compounds can exert fast nongenomic response in through their interaction with plasma membrane receptors, and activate crucial signaling pathways leading time-dependent protein tyrosine phosphorylation (TyrP). Estrogen receptor beta was detected epididymal mouse sperm; therefore, the effect 17B-estradiol, estrone, estriol,...
It has been recently shown in mice that sperm undergo acrosome reaction (AR) by passing through cumulus cells; furthermore, the acrosome-reacted can bind to zona pellucida and consequently fertilise egg. During AR, relocation of primary fusion protein IZUMO1 into equatorial segment is crucial for sperm-egg fusion. There a high rate spontaneous AR rodents, with up 60% promiscuous species. The aim this study was clarify whether after induced same, there correlation between speed...
Estrogens play a crucial role in spermatogenesis and estrogen receptor α knock-out male mice are infertile. It has been demonstrated that estrogens significantly increase the speed of capacitation vitro; however this may lead to reduction reproductive potential due decreased ability these sperm undergo acrosome reaction. To date vivo effect on capacitate not investigated. Therefore, study, we exposed (n=24) 17β-estradiol (E2) at concentration 20 ng/ml either during puberty from fourth...
Capacitation involves physiological changes that spermatozoa must undergo in the female reproductive tract or vitro to obtain ability bind, penetrate and fertilize egg. Up date, several methods have been developed characterize this complex biological process. The goal of presented study is mutually compare fluorescent techniques, check their detect molecular processes during capacitation progress determine predict percentage acrosome reacted (AR) sperm after exposure solubilized zona...
Abstract Background High-throughput studies provide a wide spectrum of genes for use as predictive markers during testicular sperm extraction (TESE) in combination with ICSI. In this work, we used the specimens from biopsies men non-obstructive azoospermia who underwent TESE to investigate expression spermatogenesis-related MND1, SPATA22, GAPDHS and ACR . Methods Testicular biopsy were subdivided into three groups: hypospermatogenesis (HS); maturation arrest (MA); Sertoli cell-only syndrome...
Male germ cells experience a drastic chromatin remodeling through the nucleo-histone to nucleo-protamine (NH-NP) transition necessary for proper sperm functionality. Post-translational modifications (PTMs) of H4 Lys5, such as acetylation (H4K5ac), play crucial role in epigenetic control nucleosome disassembly facilitating protamine incorporation into paternal DNA. It has been shown that butyrylation on same residue (H4K5bu) participates temporal regulation NH-NP mice, delaying bromodomain...
Spermatogenesis starts with the onset of puberty within seminiferous epithelium testes. It is a complex process under intricate control endocrine system. Physiological regulations by steroid hormones in general and estrogens particular are due to their chemical nature prone be disrupted exogenous factors acting as disruptors (EDs). 17α-Ethynylestradiol (EE2) an environmental pollutant confirmed ED activity well-known effect on spermatogenesis chromatin remodeling haploid germ cells. The aim...
Recent studies have shown that infertility affects estimated 15% of all couples. Male is the primary or contributory cause in 60% these cases. Consequently, application assisted reproduction increasing. These methods could benefit from an extended evaluation sperm quality. For this reason, we analyzed proteins 30 men with normal spermiograms and asthenozoospermia. Ejaculates both groups were tested by flow cytometry (FCM) fluorescence a set well-characterized anti-human Hs-monoclonal...
Juno and CD9 protein, expressed in oolemma, are known to be essential for sperm-oocyte binding fusion. Although evidence exists that these two proteins cooperate, their interaction has not yet been demonstrated. Here in, we present mutual localization over the surface of mouse metaphase II oocytes captured using 3D STED super-resolution technique. The precise examined was identified different compartments oolemma such as microvillar membrane, planar membrane between individual microvilli,...
Abstract There is a well-established link between abnormal sperm chromatin states and poor motility, however, how these two processes are interdependent unknown. Here, we identified possible mechanistic insight by showing that Protamine 2, nuclear DNA packaging protein in sperm, directly interacts with cytoskeletal Septin 12, which associated motility. 12 has several isoforms, show, the Prm2 -/- short one (Mw 36 kDa) mislocalized, while long isoforms 40 41 unexpectedly lost chromatin-bound...
In testicular germ cell tumour (TGCT) patients, sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated with low recovery rate after thawing. Since a high-energy demanding cell, which supplied by glycolysis and oxidative phosphorylation (OXPHOS), mitochondrial dysfunctionality can directly result in anomalies. this study, we investigated bioenergetic pattern of cryopreserved TGCT patients comparison normozoospermic...