A5072766518- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Rheumatoid Arthritis Research and Therapies
- Advanced Fluorescence Microscopy Techniques
- Molecular Biology Techniques and Applications
- Wound Healing and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Viral Infections and Immunology Research
- biodegradable polymer synthesis and properties
- Renal and related cancers
- Cancer, Hypoxia, and Metabolism
- Mast cells and histamine
- Image and Signal Denoising Methods
- Digital Holography and Microscopy
- bioluminescence and chemiluminescence research
- Phagocytosis and Immune Regulation
- Cytokine Signaling Pathways and Interactions
- Adipose Tissue and Metabolism
Czech Academy of Sciences, Institute of Molecular Genetics
2002-2024
Czech Academy of Sciences
1994-2014
Czech Academy of Sciences, Institute of Microbiology
1994-2004
Charles University
1994-1995
University of Pittsburgh
1994
Czech Academy of Sciences, Institute of Biotechnology
1994
UPMC Hillman Cancer Center
1994
Medical Research Council
1994
A key molecule necessary for activation of T lymphocytes through their antigen-specific cell receptor (TCR) is the transmembrane adaptor protein LAT (linker cells). Upon TCR engagement, becomes rapidly tyrosine phosphorylated and then serves as a scaffold organizing multicomponent complex that indispensable induction further downstream steps signaling cascade. Here we describe identification preliminary characterization novel structurally evolutionarily related to expressed in B lymphocytes,...
Glyco-coat changes on cancer cells due to aberrant glycosylation are potential targets for immune recognition through lectin-like receptors cells. These include natural killer (NK), CD8+ and CD4+ lymphocytes, all reported have, together with cytokines, important functions in antitumor immunity. The aim of this study was evaluate a possible role synthetic monodisperse multivalent neo-glycoconjugates, namely glycodendrimers, as new approach anticancer modulation carbohydrate-mediated...
Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. becomes tyrosine phosphorylated after cross-linking the CD4 or CD8 coreceptors. Phospho-LIME associates with Src family kinase Lck and its negative regulator, Csk. Ectopic expression Jurkat cells results an increase Csk lipid rafts, increased phosphorylation higher...
Abstract Natural killer receptor protein 1 (NKR-P1, a family of proteins), which is dimeric transmembrane predominantly on rat and murine natural cells, contains an extracellular motif related to calcium-dependent animal lectins. The domain architecture this the finding that its cross-linking with antibody results in activation cells make it promising candidate for function. We have expressed full-length NKR-P1 COS prepared soluble fragments by controlled proteolysis or expression truncated...
NTAL (non-T-cell activation linker, also called LAB) and LAT (linker for of T cells) are evolutionarily related transmembrane adaptor proteins that phosphorylated upon immunoreceptor engagement. Using quantitative reverse transcription-PCR, both were found to be expressed in B cells. However, expression was limited early cells, whereas typified mature To delineate their roles B-cell development function, Ntal-deficient mice generated crossed with Lat-deficient mice. cells developed Lat(-/-)...
Vitronectin is an abundant plasma glycoprotein identified also as a part of extracellular matrix. substantially enriched at sites injured, fibrosing, inflamed, and tumor tissues where it believed to be involved in wound healing tissue remodeling. Little known about the mechanism vitronectin localization into damaged tissues.2E12 antibody has been described bind subset late apoptotic cells. Using immunoisolation followed by mass spectrometry, we antigen recognized 2E12 vitronectin. Based on...
Glyco-coat changes on cancer cells due to aberrant glycosylation are potential targets for immune recognition through lectin-like receptors cells. These include natural killer (NK), CD8+ and CD4+ lymphocytes, all reported have, together with cytokines, important functions in antitumor immunity. The aim of this study was evaluate a possible role synthetic monodisperse multivalent neo-glycoconjugates, namely glycodendrimers, as new approach anticancer modulation carbohydrate-mediated...
Glycosphingolipid-enriched microdomains (GEM) are membrane entities that concentrate glycosylphosphatiolylinositol(GPI)-anchored, acylated and proteins important for immune receptor signaling. Using rat leukemic cell line RNK-16 we have initiated proteomic studies of in natural killer (NK) cells. Isolated plasma membranes were treated with Brij 58, or Nonidet-P40, sodium carbonate. Extracts separated by sucrose density gradient centrifugation into very light membrane, medium heavy fractions,...
A firefly luciferase (FLuc) counts among the most popular reporters of present-day molecular and cellular biology. In this study, we report a cryptic promoter activity in luc+ gene, which is frequently used version luciferase. The FLuc coding region displays both mammalian yeast cells. human CCL13 Huh7 cells, transcription from gene 10–16 times weaker comparison to strong immediate-early cytomegalovirus promoter. Additionally, discuss possible impact on experimental results especially some...
Abstract TCR gene rearrangement generates diversity of T lymphocytes by V(D)J recombination. Ig genes are rearranged in B cells using the same enzyme machinery. TCRD (TCR δ) frequently incompletely precursor leukemias and recently were found a significant portion physiological lymphocytes. Incomplete rearrangements (V-D) thus serve as natural indicators previous recombinase activity. Functional has been murine NK precursors. We tested whether other leukocyte subpopulations contained humans....
Abstract TCR gene rearrangement generates diversity of T lymphocytes by V(D)J recombination. Ig genes are rearranged in B cells using the same enzyme machinery. Physiologically, is postulated to rearrange exclusively lineage, but malignant precursor lymphoblasts contain most patients. Several mechanisms which break regulation recombination have been proposed. In this study we show that incomplete δ rearrangements V2-D3 and D2-D3 occur each up 16% alleles all healthy donors studied, complete...
Natural killer (NK) and T cells express a superfamily of proteins with structural features C-type lectins. Recombinantly prepared soluble form rat NKR-P1 (CD161) recognized carbohydrate GalNac GlcNac moieties. Ganglioside GM2 heparin related-IS oligosaccharides representing the high affinity ligands for this receptor, increased sensitivity targets killing by effectors isolated from blood spleen in vitro. Based on these results, we investigated vivo possible therapeutic effect IS carried...
ABSTRACT Super-resolution (SR) microscopy is a cutting-edge method that can provide detailed structural information with high resolution. However, the thickness of specimen has been major limitation for SR methods, and larger structures have posed challenge. To overcome this, key step to optimize sample preparation ensure optical homogeneity clarity, which enhance capabilities methods acquisition thicker structures. Oocytes are largest cells in mammalian body crucial objects reproductive...
Juno and CD9 protein, expressed in oolemma, are known to be essential for sperm-oocyte binding fusion. Although evidence exists that these two proteins cooperate, their interaction has not yet been demonstrated. Here in, we present mutual localization over the surface of mouse metaphase II oocytes captured using 3D STED super-resolution technique. The precise examined was identified different compartments oolemma such as microvillar membrane, planar membrane between individual microvilli,...
Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims this study were to further characterize the involvement UCP2 hematopoiesis. In situ hybridization mouse embryos identified UCP2‐positive liver inside primitive blood vessels from 10.5 days prenatal development. High transcript levels detected reticulocytes other maturating erythroid peripheral mice exposed...
Hepatitis C virus (HCV) is an important pathogen causing both acute and chronic infections in humans. The HCV polyprotein synthesized by cap-independent translation initiation after ribosome binding to the highly structured internal entry site (IRES). IRES has been shown have a low requirement for factors ability bind directly 40S ribosomal subunit. A novel yeast bicistronic reporter system, suitable sensitive accurate analysis of activity, developed. It employs signal amplification based on...
Satellite cells represent a heterogeneous population of stem and progenitor responsible for muscle growth, repair regeneration. We investigated whether c-Myb could play role in satellite cell biology because our previous results using cell-derived mouse myoblast line C2C12 showed that was expressed growing downregulated during differentiation. detected expression activated regenerating muscle. also discovered associated with isolated viable myofiber descendants cells, proliferating...
Abstract Super‐resolution (SR) microscopy is a cutting‐edge method that can provide detailed structural information with high resolution. However, the thickness of specimen has been major limitation for SR methods, and large biological structures have posed challenge. To overcome this, key step to optimise sample preparation ensure optical homogeneity clarity, which enhance capabilities methods acquisition thicker structures. Oocytes are largest cells in mammalian body crucial objects...
Filtration of super-resolved microscopic images brings often troubles with removing undesired image parts like, e.g., noise, inhomogenous background and reconstruction artifacts. Standard filtration techniques, convolution- or Fourier transform-based methods are not always appropriate, since they may lower resolution that was acquired by hi-tech expensive microscopy systems. Thus, in this article it is proposed to filter such using discrete wavelet transform (DWT). Newly developed...
We discuss the presence of anti-keratin antibodies (AKA) IgG class in patients with defined juvenile idiopathic arthritis (JIA).An indirect immunofluorescence test and rat oesophagus substrate was used for detection quantification AKA patients´ sera.Overall 33/60 JIA had sera positive (55 %, P = 0,0001) ranging from 1:10 to 1:160 dilutions.Following childhood classification criteria occurred 2/7 systemic disease (28,6 %), 13/30 RF negative polyarthritis (43,3 0,008) 15/18 (83,3 0,000002).AKA...
Vascular changes are consistent early findings in patients with SSc and often precede the development of fibrosis.Despite a significant reduction capillary density, there is paradoxically no sufficient angiogenesis skin patients.By using pO 2 histograph, we showed that low values overt involved SSc.In vitro, real-time PCR revealed 3.7-fold upregulation potent angiogenic growth factor VEGF fibroblasts after hypoxic exposure compared to normoxic controls.In situ hybridization for biopsies an...