A5045949979- Sperm and Testicular Function
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Reproductive System and Pregnancy
- Diabetes and associated disorders
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Molecular Biology Techniques and Applications
- Complement system in diseases
- Animal Behavior and Reproduction
- Metabolism and Genetic Disorders
- Advanced Fluorescence Microscopy Techniques
- Renal and related cancers
- Drug Transport and Resistance Mechanisms
- Tissue Engineering and Regenerative Medicine
- Fish Ecology and Management Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Nuclear Structure and Function
- Genetic diversity and population structure
- Signaling Pathways in Disease
- Extracellular vesicles in disease
- Platelet Disorders and Treatments
- Olfactory and Sensory Function Studies
- Photochromic and Fluorescence Chemistry
Czech Academy of Sciences, Institute of Biotechnology
2016-2024
Czech Academy of Sciences
2018-2020
Charles University
2009-2012
Proteins CD9 and CD81 are members of the tetraspanin superfamily were detected in mammalian sperm, where they suspected to form an active web participate sperm–egg membrane fusion. The importance these two proteins during early stages fertilization is supported by complete sterility CD9/CD81 double null female mice. In this study, putative mechanism involvement formation sperm its activity prior was addressed. Confocal microscopy colocalization assay used determine a mutual localization...
Gamete fusion is a critical event of mammalian fertilization. A random one-bead one-compound combinatorial peptide library represented synthetic human egg mimics and identified previously unidentified ligand as Fc receptor–like 3, named MAIA after the mythological goddess intertwined with JUNO. This immunoglobulin super family receptor was expressed on oolemma played major role during sperm-egg adhesion fusion. forms highly stable interaction known IZUMO1/JUNO complex, permitting specific...
The acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. It widely recognized that specific proteins form an active network prior fertilization, their dynamic relocation crucial for sperm-egg fusion. unique presence cofactor protein CD46 in acrosomal was shown, however, its behaviour connection with other has not been explored further. Using super resolution microscopy, we demonstrated reorganisation over head...
Sperm–egg interaction and fusion represent a key moment of fertilization. In mammals, it is not possible without the tetraspanin superfamily proteins including CD81. A detailed immunohistochemical localization CD81 was monitored in bovine oocytes during different maturation stages, as well early embryogenesis. addition, characterization carried out mouse sperm. eggs, detected on plasma membrane germinal vesicle, metaphase I II oocytes. During fertilization, accumulation molecules...
The long-term storage of boar sperm presents an ongoing challenge, and the modification cryoprotective compounds in semen extenders is crucial for improving cryopreservation’s success rate. aim our study was to reduce percentage glycerol extender by elimination or substitution with biocompatible, non-toxic polysaccharides. For improvement, we tested a novel polysaccharides dextran pentaisomaltose combination unique silico predictive modeling. We targeted analysis vitro qualitative parameters...
In rodents, the cell surface complement regulatory protein CD46 is expressed solely on spermatozoal acrosome membrane. Ablation of gene associated with a faster reaction. Sperm from Apodemus flavicollis (yellow-necked field mice), A. microps (pygmy mice) and sylvaticus (European wood fail to express exhibit more rapid reaction rate than Mus (house or BALB/c mice. agrarius (striped belong different subgenus have pronounced promiscuity large relative testis size. The aim this study was...
The physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 CD81, are involved in sperm-egg membrane fusion, similarly to integrins, they occupy characteristic regions. We report here on a newly discovered presence sperm, present expression distribution during spermatogenesis sperm transition the acrosome reaction. traced gene protein testicular cell subpopulations, with...
The acrosome located within the mammalian sperm head is essential for successful fertilization, as it enables to penetrate extracellular layers of oocyte and fuse with oolemma. However, acrosomal vesicle no longer considered contain only hydrolytic enzymes. Using label-free nano-scale liquid chromatography tandem mass spectrometry (nLC-MS/MS) proteomics, we identified a total 885 proteins in isolated from spermatozoa obtained cauda epididymis free-living house mice Mus musculus contains...
Integrins are transmembrane cell receptors involved in two crucial mechanisms for successful fertilization, namely, mammalian intracellular signaling and adhesion. α6β4, α3β1 α6β1 three major laminin expressed on the surface of cells including gametes, presence individual integrin subunits α3, α6, β1 β4 has been previously detected sperm. However, to date, proof existence heterodimer pairs sperm their detailed localization is missing. The conclusion this study evidence that subunit mouse it...
Spermatogenesis starts with the onset of puberty within seminiferous epithelium testes. It is a complex process under intricate control endocrine system. Physiological regulations by steroid hormones in general and estrogens particular are due to their chemical nature prone be disrupted exogenous factors acting as disruptors (EDs). 17α-Ethynylestradiol (EE2) an environmental pollutant confirmed ED activity well-known effect on spermatogenesis chromatin remodeling haploid germ cells. The aim...
Fertilization is a multiple step process leading to the fusion of female and male gametes formation zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte sperm surface, fertilization also involves communication chemical molecules triggering various signaling pathways. This work focuses mouse taste receptor, mTAS1R3, encoded by Tas1r3 gene, as potential receptor mediating between using C57BL/6J lab strain. In order specify role we aimed characterize...
Abstract Divergence in sperm phenotype and female reproductive environment may be a common source of postmating prezygotic (PMPZ) isolation between species. However, compared to other barriers it has received much less attention. In this study, we examined morphology velocity two hybridizing passerine species, the nightingale ( Luscinia megarhynchos ) thrush L. luscinia ). addition, for first time characterized tract fluid proteome. We demonstrate that spermatozoa have significantly longer...
In humans, CD46 has been detected on the acrosomal membrane in sperm, contrast to widespread surface expression somatic cells where it plays a key role protection from complement attack. rodents, is expressed solely of mature sperm and their immediate precursors, spermatids. A monoclonal antibody against short consensus repeat (SCR1) ectodomain blocks binding human zona-free oocytes vitro. However, CD46-knockout mice are fertile have an accelerated spontaneous acrosome reaction. Wild-caught...
ABSTRACT Super-resolution (SR) microscopy is a cutting-edge method that can provide detailed structural information with high resolution. However, the thickness of specimen has been major limitation for SR methods, and larger structures have posed challenge. To overcome this, key step to optimize sample preparation ensure optical homogeneity clarity, which enhance capabilities methods acquisition thicker structures. Oocytes are largest cells in mammalian body crucial objects reproductive...
Juno and CD9 protein, expressed in oolemma, are known to be essential for sperm-oocyte binding fusion. Although evidence exists that these two proteins cooperate, their interaction has not yet been demonstrated. Here in, we present mutual localization over the surface of mouse metaphase II oocytes captured using 3D STED super-resolution technique. The precise examined was identified different compartments oolemma such as microvillar membrane, planar membrane between individual microvilli,...
Integrins are transmembrane receptors that facilitate cell adhesion and cell-extracellular matrix communication. They involved in the sperm maturation including capacitation gamete interaction, resulting successful fertilization. αV integrin belongs to glycoprotein superfamily, it is indispensable for physiological spermiogenesis testosterone production. We targeted gene protein expression of subunit described its membrane localization sperm. Firstly, mouse, we traced during spermatogenesis...
Abstract There is a well-established link between abnormal sperm chromatin states and poor motility, however, how these two processes are interdependent unknown. Here, we identified possible mechanistic insight by showing that Protamine 2, nuclear DNA packaging protein in sperm, directly interacts with cytoskeletal Septin 12, which associated motility. 12 has several isoforms, show, the Prm2 -/- short one (Mw 36 kDa) mislocalized, while long isoforms 40 41 unexpectedly lost chromatin-bound...
Abstract Super‐resolution (SR) microscopy is a cutting‐edge method that can provide detailed structural information with high resolution. However, the thickness of specimen has been major limitation for SR methods, and large biological structures have posed challenge. To overcome this, key step to optimise sample preparation ensure optical homogeneity clarity, which enhance capabilities methods acquisition thicker structures. Oocytes are largest cells in mammalian body crucial objects...
There is a well-established link between abnormal sperm chromatin states and poor motility, however, how these two processes are interdependent unknown. Here, we identified possible mechanistic insight by showing that Protamine 2, nuclear DNA packaging protein in sperm, directly interacts with cytoskeletal Septin 12, which associated motility. 12 has several isoforms, show, the