A5076379269- Gene Regulatory Network Analysis
- Developmental Biology and Gene Regulation
- bioluminescence and chemiluminescence research
- Model Reduction and Neural Networks
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- Protein Kinase Regulation and GTPase Signaling
- Cellular Mechanics and Interactions
- Protein Structure and Dynamics
- Cell Image Analysis Techniques
- Machine Learning in Materials Science
- Epigenetics and DNA Methylation
- Biofuel production and bioconversion
- Melanoma and MAPK Pathways
- Behavioral Health and Interventions
- Protein Tyrosine Phosphatases
- Receptor Mechanisms and Signaling
- Evolution and Genetic Dynamics
- Computational Drug Discovery Methods
- Physical Activity and Health
- Fungal and yeast genetics research
- Fibroblast Growth Factor Research
- Microbial Metabolic Engineering and Bioproduction
- Hippo pathway signaling and YAP/TAZ
- Probabilistic and Robust Engineering Design
Princeton University
2020-2023
Maryville College
2021
University of Alabama
2015
Significance In early development, cells commit to a single germ fate despite receiving multiple, conflicting inductive cues. Here, we examine how in the Drosophila embryo integrate promesodermal and proendodermal signals. We find that proendoderm signals repress transcriptional determinants of mesodermal cell movements during critical time window embryo. Based on precise optogenetic perturbations, live imaging, computational modeling, our work provides framework for quantitative...
We present a data-driven approach to characterizing nonidentifiability of model's parameters and illustrate it through dynamic as well steady kinetic models. By employing Diffusion Maps their extensions, we discover the minimal combinations required characterize output behavior chemical system: set effective for model. Furthermore, introduce use Conformal Autoencoder Neural Network technique, kernel-based Jointly Smooth Function disentangle redundant parameter that do not affect from ones...
Positional information in development often manifests as stripes of gene expression, but how form remains incompletely understood. Here, we use optogenetics and live-cell biosensors to investigate the posterior brachyenteron (byn) stripe early Drosophila embryos. This depends on interpretation an upstream ERK activity gradient expression two target genes, tailless (tll) huckebein (hkb), that exert antagonistic control over byn. We find high or low doses signaling produce transient sustained...
Abstract Cells employ intracellular signaling pathways to sense and respond changes in their external environment. In recent years, live-cell biosensors have revealed complex pulsatile dynamics many pathways, but studies of these are limited by the necessity imaging at high spatiotemporal resolution 1 . Here, we describe an approach infer from just a single measurement fixed cells using pulse-detecting gene circuit. We computationally screened for circuit with pulse detecting capability,...
The Extracellular signal Regulated Kinase (ERK) controls multiple critical processes in the cell and is deregulated by mutations human cancers, congenital abnormalities, immune diseases, neurodevelopmental syndromes. Catalytic activity of ERK requires dual phosphorylation an upstream kinase, a mechanism that can be described two sequential Michaelis-Menten (MM) steps. estimation individual reaction rate constants from kinetic data full has proved challenging. Here, we present analytically...
We present a data-driven approach to characterizing nonidentifiability of model's parameters and illustrate it through dynamic as well steady kinetic models. By employing Diffusion Maps their extensions, we discover the minimal combinations required characterize output behavior chemical system: set effective for model. Furthermore, introduce use Conformal Autoencoder Neural Network technique, kernel-based Jointly Smooth Function disentangle redundant parameter that do not affect from ones...
Exercise is Medicine–On Campus (EIM-OC) involves promoting physical activity on college campuses and ideally integrated into the student health clinic to assess PA provide exercise prescriptions. PURPOSE: To evaluate awareness, engagement, determinants of participation in EIM-OC which housed Health Science Outdoor Studies (HSOS) Division at a small liberal arts (1148 students). METHODS: Maryville College students completed an anonymous, online survey asking about their knowledge PA....
Positional information in developing tissues often takes the form of stripes gene expression that mark boundaries a particular cell type or morphogenetic process. How is still many cases poorly understood. Here we use optogenetics and live-cell biosensors to investigate one such pattern: posterior stripe brachyenteron (byn) early Drosophila embryo. This byn depends on interpretation an upstream signal - gradient ERK kinase activity two target genes tailless (tll) huckebein (hkb) exert...