The use of biofertilizers is important for sustainable agriculture, and the nodule bacteria endophytic actinomycetes an attractive way to enhance plant growth yield. This study tested effects a biofertilizer produced from Bradyrhizobium strains Streptomyces griseoflavus on leguminous, cereal, vegetable crops. Nitrogen fixation was measured using acetylene reduction assay. Under N-limited or N-supplemented conditions, significantly promoted shoot root mung bean, cowpea, soybean compared with...

We investigated the effects of integrated organic and inorganic fertilizers on growth yield indica rice variety Manawthukha japonica Genkitsukushi. In a split-plot design, two varieties were assigned as main plot factors, treatments subplot including no-N fertilizer (N0), 50% chemical (CF) (CF50), 100% CF (CF100), + poultry manure (PM) (CF50PM50), cow (CM) (CF50CM50), compost (CP) (CF50CP50). CF100 was equivalent to N at 85 kg/hm2. Manure applied based estimated mineralizable nitrogen (EMN)...

The decline in rice yields as a result of excessive chemical fertilizer (CF) inputs is matter great concern rice-growing regions Asia. In two-year’s field experiments, the nitrogen, phosphorus, and potassium (NPK) status, growth characteristics yield were examined by application poultry manure (PM), cow (CM) compost (CP). Organic fertilizers applied EMN (estimated mineralizable N) based on their total N content. Six treatments assigned randomized complete block design: (1) no-N (N0); (2) 50%...

ABSTRACT Co-inoculation of nitrogen-fixing bacteria with plant growth-promoting has become more popular than single inoculation rhizobia or plant-growth-promoting because the synergy these in increasing soybean yield and nitrogen fixation. This study was conducted to investigate effects Bradyrhizobium japonicum SAY3-7 Streptomyces griseoflavus P4 co-inoculation on growth, nodulation, fixation, nutrient uptake, seed 'Yezin-6' cultivar. Nitrogen fixation measured using acetylene reduction...

This study was conducted to evaluate the effects of Bradyrhizobium japonicum SAY3-7, elkanii BLY3-8, and Streptomyces griseoflavus P4 on symbiotic effectiveness soybeans before biofertilizer production, produce containing studied three strains (SAY3-7, P4), test soybean varieties, assess varietal interaction between variety plant growth, nodulation, nitrogen fixation, nutrient absorption, seed yield. Nitrogen fixation measured using acetylene reduction assay ureide methods. Contents...

Drought is one of the main factors limiting rice (Oryza sativa L.) productivity and has become an increasingly severe problem in many regions worldwide. Establishing breeding programs to develop new drought-tolerant varieties requires understanding effect drought on plants mechanisms tolerance rice. We conducted a pot experiment explore growth characteristics, root plasticity, stomatal conductance six (DA8, Malagkit Pirurutong, Thierno Bande, Pate Blanc MN1, Kinandang Patong, Moroberekan)...

Chickpea (Cicer arietinum L.) is one of the world’s main leguminous crops that provide chief source food for humans. In present study, we characterized thirty isolates indigenous chickpea rhizobia from Myanmar based on sequence analysis bacterial 16S rRNA gene. The confirmed all were categorized and identified as genus Mesorhizobium they conspecific with M. plurifarium, muliense, tianshanense, sp. This first report describing plurifurium different geographical distribution mesorhizobia in...

Pueraria is an herbaceous, perennial legume crop originating in Asia. phaseoloides (Roxb.) Benth. (tropical kudzu) frequently introduced into production systems and used as green manure, a cover forage plant, making it important economically. We P. trap to study characterize soil rhizobia Eastern Cameroon. Bacteria were isolated from fresh nodules collected field-grown roots. The 16S-23S rRNA internal transcribed spacer (ITS) sequences 30 bacterial isolates amplified by polymerase chain...

Co-inoculation of selected nitrogen-fixing bacteria with plant growth-promoting is the promising way for improvement soybean production through enhancing growth, nodulation, and N2 fixation. Therefore, this experiment was conducted to study effects co-inoculation Bradyrhizobium elkanii BLY3-8 Streptomyces griseoflavus P4 on fixation, N uptake, seed yield Rj4 varieties. Two experiments completely randomized design three replicates were done in study. N2-fixation ability evaluated by acetylene...

The nitrogen (N) uptake, growth characteristics, and yield of the Genkitsukushi rice variety were evaluated over 2 years (2017 2018) field experiments involving application poultry manure (PM), cow (CM), compost (CP). Organic fertilizers quantified as estimated-mineralizable-nitrogen (EMN) based on total N content. Compared with chemical fertilizer (CF100), CF50PM50 resulted in greater characters, dry matter, both years. PM (total > 4%) had a higher mineralization, availability to thus,...

Soybean (Glycine max L.) plays an essential role in human nutrition as a protein source, and plant N source. The rate of fixation varies depending on the cultivars compatibility between inoculated Rhizobium strain host cultivar. Characterizing nodulation regulatory (Rj) genes is necessary to determine strains. Rj were previously identified based inoculation tests PCR analyses. six Yezin-3, Yezin-7, Yezin-11, Shan Seine (Local), Madaya Hinthada (Local) harboring Rj4 gene. Two cultivars,...

Inoculation density has a marked effect on nodulation and N fixation in soybean (Glycine max L.). Therefore, we conducted this study to determine the optimal inoculation of Bradyhizobium japonicum SAY3-7 (SAY3-7) Streptomyces griseoflavus P4 (P4) for plant growth, nodulation, fixation, investigate co-inoculation selected cultivars, using density. Nitrogen terms an acetylene reduction activity value, was measured flame ionization gas chromatograph. In study, low-density single with (105 or...

Bradyrhizobium elkanii BLY3-8 does not form nodules on the roots of Rj3-genotype soybean (cultivar D-51). This is a cultivar-specific nodulation restriction. The genes A6X20_40975 and A6X20_41030 in strain were predicted to encode transcriptional activator apparatus type III secretion system (T3SS) (the proteins TtsI RhcJ), respectively. Mutants disrupted these overcame These results suggest that an effector injected via T3SS into involved restriction by soybean.

We report here the draft genome sequences of Bradyrhizobium elkanii strains BLY3-8 and BLY6-1, which are incompatible with Rj3 genotype soybean cultivars. The these will be useful to identify a causal gene for this incompatibility.

Bradyrhizobia are known symbiotic partners of soybean. However, some soybean cultivars restrict nodulation by Bradyrhizobium bacterial strains. These restrictions related to compatibility between the Rj genes and types inoculated bacteria. The objective this study was determine incompatibility Type B strains with Rj3 cultivars. Newly isolated B. elkanii BLY3-8 BLY6-1 from Myanmar specific strain USDA33, which incompatible cultivars, japonicum USDA110 were used as inoculants check or Nitrogen...

Abstract We collected soil samples from six major mung bean cropping regions in Myanmar: Sagaing, Mandalay, Nay Pyi Taw, and Magway the tropical savanna climate zone Bago Yangon monsoon zone. All fields grew for at least 5 years had no history of rhizobial inoculation. Mung ‘Yezin-11’, a popular cultivar Myanmar, was inoculated with suspensions. From nodules formed on roots, we isolated 55 strains. Identification isolates revealed dominant species indigenous rhizobia each region. identified...

(2019). Nitrate, nutrient content and growth parameters of komatsuna (Brassica rapa L.) in response to manure application depending on EMN (estimated mineralizable nitrogen) Journal Plant Nutrition: Vol. 42, No. 15, pp. 1726-1739.

We investigated the effects of fermented nori (Pyropia yezoensis) liquid fertilizers on plant growth, soil analysis development (SPAD) values, and nutrient uptake komatsuna (Brassica rapa L. var. wakana komatsuna) plants. The four types seaweed (SLFs) evaluated in this study were prepared by anaerobic fermentation unwashed (SLF1), aerobic (SLF2), washed (SLF3), (SLF4). In Experiment 1, highest SPAD, values obtained from treatment with SLF2 SLF4. There no significant differences between basal...

Black gram (Vigna mungo L. Hepper) is one of the main leguminous crops that provide chief source food. Several Bradyrhizobium species are able to induce effective nodules in black cultivars. In present study, we characterized forty isolates indigenous bradyrhizobia from Myanmar based on sequence analysis bacterial 16S rRNA gene. The confirmed all were categorized and identified as genus they conspecific with B. elkanii, sp., liaoningense, japonicum yunamingense. Almost collected major...

We conducted plant growth experiments in microbe-free vermiculite to study the effects of four types fermented seaweed liquid fertilizer (SLF) made from nori (Pyropia yezoensis) on germination, characteristics, SPAD value, and nutrient content uptake komatsuna (Brassica rapa L. var. wakana komatsuna). The SLF used this were prepared by anaerobic fermentation unwashed (SLF1), aerobic (SLF2), washed (SLF3), (SLF4). Komatsuna seeds treated with 200-, 300-, 400-fold dilutions SLFs exhibited...

The selection of effective rhizobia for higher efficiency nitrogen fixation is one the most important steps inoculant production. Therefore, this experiment was conducted to select type A and B strains specific Rj-gene harboring soybean varieties test symbiotic effectiveness selected on different varieties. Screening experiments using were done with a completely randomized design three replications in study. Evaluation Myanmar Bradyrhizobium plant growth, nodulation N2 studied pot sterilized...

Bradyrhizobium species are symbiotic partners of soybean plants. However, some bacteria do not form functional nodules on the roots Rj4 genotype cultivars. Our objective was to identify strains (i.e., type C strains) that least competent this plant genotype. We checked (i) previously isolated Myanmar elkanii (MMY6-1, MMY6-2, and MMY6-5), (ii) Myanmarese spp. (MMY3-5 MMY3-7), (iii) strain Is-34 B. japonicum, for nodule formation when associated with other Rj soybeans. Strains in groups (i),...

Two field experiments were conducted at Yezin farm and Sepin research farm, Yamenthin, Myanmar to investigate the effect of different mulching materials on growth, soil moisture weed infestation maize identify most
 farms, Yamethin, most suitable for cultivation in study areas during dry season (October, 2019 March, 2020). The used randomized complete block design (RCB) with three replications. No six materials, including rice straw mulching, husk stover mung bean soybean white plastic...