A5057814149- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Polysaccharides and Plant Cell Walls
- Advanced Materials Characterization Techniques
- RNA Interference and Gene Delivery
- Advanced Cellulose Research Studies
- High Temperature Alloys and Creep
- Biofuel production and bioconversion
- Plant nutrient uptake and metabolism
- RNA and protein synthesis mechanisms
- Additive Manufacturing Materials and Processes
- Hydrogen embrittlement and corrosion behaviors in metals
- Bacteriophages and microbial interactions
- Polymer Surface Interaction Studies
- Connective tissue disorders research
- Dendrimers and Hyperbranched Polymers
- Molecular Junctions and Nanostructures
- Metal complexes synthesis and properties
- Aluminum toxicity and tolerance in plants and animals
- ZnO doping and properties
- Traffic Prediction and Management Techniques
- Data Visualization and Analytics
- Material Selection and Properties
- Silk-based biomaterials and applications
- Characterization and Applications of Magnetic Nanoparticles
North Carolina State University
2013-2024
Yerevan State University
2024
North Central State College
2024
Purdue University West Lafayette
2024
Smith-Kettlewell Eye Research Institute
2021
Massachusetts Eye and Ear Infirmary
2021
Ganesh Shankar Vidyarthi Memorial Medical College
2019
Columbia University
2017
University of North Texas
2009-2014
Samsung (United States)
2013
Abstract A six-lobed membrane spanning cellulose synthesis complex (CSC) containing multiple synthase (CESA) glycosyltransferases mediates microfibril formation. The number of CESAs in the CSC has been debated for decades light changing estimates diameter smallest formed from β-1,4 glucan chains synthesized by one CSC. We obtained more direct evidence through generating improved transmission electron microscopy (TEM) images and image averages rosette-type CSC, revealing frequent...
Plants are constantly subjected to various biotic and abiotic stresses have evolved complex strategies cope with these stresses. For example, plant cells endocytose plasma membrane material under stress subsequently recycle it back when the conditions relieved. Cellulose biosynthesis is a tightly regulated process that performed by membrane-localized cellulose synthase (CESA) complexes (CSCs). However, regulatory mechanism of has not been well explored. In this study, we show small CESA...
Weakly charged cationic nanoparticles cause structural changes including local denaturing and compaction to DNA under mild conditions. The ligands bind the phosphate backbone of uncharged penetrate helix disrupt base pairing. Mobility shifts in electrophoresis, molecular dynamics, UV-vis spectrophotometry give clues details interactions. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They...
Single-stranded DNA (ssDNA) plays an important role in biological processes and is used nanotechnology other novel applications. Many research questions can be addressed with molecular simulations of ssDNA molecules; however, no dedicated force field for has been developed, there limited experimental information about structures. This study assesses the accuracy applicability existing Amber fields all-atom ssDNA, such as ff99, bsc0, bsc1, OL15, implicit explicit solvents via comparison to...
Cellulose synthases (CESAs) are glycosyltransferases that catalyze formation of cellulose microfibrils in plant cell walls. Seed CESA isoforms cluster six phylogenetic clades, whose non-interchangeable members play distinct roles within synthesis complexes (CSCs). A 'class specific region' (CSR), with higher sequence similarity versus between functional classes, has been suggested to contribute activities or interactions different isoforms. We investigated isoform specificity the moss,...
The development of nucleic acid (NA) based nanotechnology applications rely on the efficient packaging DNA and RNA. However, atomic details NA-nanoparticle binding remains to be comprehensively characterized. Here, we examined how nanoparticle solvent properties affect NA compaction. Our large-scale, all-atom simulations ligand-functionalized gold (NP) double stranded NAs as a function NP charge solution salt concentration reveal different responses RNA cationic NPs. We demonstrate that...
Plant growth and survival depend upon the activity of membrane transporters that control movement distribution solutes into, around, out plants. Although many plant are known, their intrinsic properties make them difficult to study. In barley (Hordeum vulgare), root anion-permeable transporter Bot1 plays a key role in tolerance high soil boron, facilitating efflux borate from cells. However, its three-dimensional structure is unavailable molecular basis permeation function unknown. Using an...
DNA strand length has been found to be an important factor in many DNA-based nanoscale systems. Here, we apply molecular dynamics simulations a synergistic effort with layer-by-layer experimental data understand the effect of on assembly films. The results indicate that short (less than 10 bases) and long (more 30 single-stranded DNAs do not exhibit optimal film growth, this can associated limited accessibility bases surface due formation self-protected interactions prevent efficient...
Transition-metal dopants play a critical role in the high-temperature mechanical strength and corrosion resistance of nickel-based superalloys. In this article, site occupancy behavior chromium γ′-Ni3Al has been investigated by combining three-dimensional (3D) atom probe high-resolution transmission electron microscopy characterizations with ab initio density functional theory (DFT) calculations. The 3D data show clear preference on aluminum sublattice over nickel Rene88 super alloys....
Assembling robust chiral biopolymer structures without compromising vivid optical iridescence is a grand challenge for biocomposite materials. Herein, we report hierarchical nanocellulose nanostructure with helicoidal organization co-assembled from rigid cellulose nanocrystals (CNCs) and longer nanofibers isolated the hydrolyzed wood pulp. This resulting highly iridescent material much tougher than traditional CNC films. We found that mixed are composed of needle-like very long (up to 800...
The processivity of cellulose synthesis in bacterial synthase (CESA) was investigated using molecular dynamics simulations and the hybrid quantum mechanics approach. Our results suggested that CESA can be initiated with H2O molecules. chain length or degree polymerization (DOP) product is related to affinity transmembrane tunnel enzyme. This opens up possibility generating mutants would produce chains desired lengths could have applications biofuel textile fields depend on DOP chains.
Sequence dependency of metal ion aggregation around RNA structures is known to be involved in critical functions ranging from processes molecular recognition enzymatic chemistry. Ion interactions with an HIV-1 TAR core helix were examined explicit solvent dynamics simulations. The results have shown that there a sequence-dependent cationic localization toward the purine-rich run within and other duplexes. behavior independent ionic species or presence bulge. A region high affinity agrees...
Abstract The molecular recognition properties of DNA gave rise to many novel materials and applications such as biosensors, DNA‐functionalized colloidal materials, origami DNA‐based directed surface assembly. surfaces are used in biosensors for programmed self‐assembly biological, organic inorganic moieties into materials. However, density, length, linker design the functionalized DNAs significantly influence DNA‐driven assemblies This perspective discusses understanding structure dynamics...
Higher plants synthesize cellulose using membrane-bound, six-lobed synthase complexes, each lobe containing trimeric synthases (CESAs). Although molecular biology reports support heteromeric trimers composed of different isoforms, a homomeric trimer was reported for in vitro studies the catalytic domain CESA1 Arabidopsis (AtCESA1CatD) and confirmed cryoEM structures full-length CESA8 CESA7 poplar cotton, respectively. In both structures, small portion plant-conserved region (P-CR) forms only...