A5071030090- Polysaccharides and Plant Cell Walls
- Advanced Cellulose Research Studies
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Cellular transport and secretion
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Endoplasmic Reticulum Stress and Disease
- Insect and Arachnid Ecology and Behavior
- Light effects on plants
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Spectroscopy and Chemometric Analyses
- Genetic Mapping and Diversity in Plants and Animals
- Cholesterol and Lipid Metabolism
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Remote Sensing in Agriculture
- Plant Stress Responses and Tolerance
North Carolina State University
2013-2021
Michigan State University
2008-2018
Abstract A six-lobed membrane spanning cellulose synthesis complex (CSC) containing multiple synthase (CESA) glycosyltransferases mediates microfibril formation. The number of CESAs in the CSC has been debated for decades light changing estimates diameter smallest formed from β-1,4 glucan chains synthesized by one CSC. We obtained more direct evidence through generating improved transmission electron microscopy (TEM) images and image averages rosette-type CSC, revealing frequent...
In plants, sterols play fundamental roles as membrane constituents in the biosynthesis of steroid hormones, and act precursors for cell wall deposition. Sterols are synthesized endoplasmic reticulum (ER), but mainly accumulate plasma membrane. How trafficked plant cells is largely unknown. non-plant systems, oxysterol-binding proteins have been involved sterol trafficking homeostasis. There at least twelve homologs Arabidopsis genome, biology these remains most part obscure. Here, we report...
Eukaryotic cells internalize cargo at the plasma membrane via endocytosis, a vital process that is accomplished through complex network of endosomal organelles. In mammalian cells, ER in close association with endosomes and regulates their fission. Nonetheless, physiological role such interaction on endocytosis yet unexplored. Here, we probed existence ER-endosome plant assayed its endocytosis. Through live-cell imaging electron microscopy studies, established are extensively associated ER,...
The circadian clock controls many molecular activities, impacting experimental interpretation. We quantify the genome-wide effects of time-of-day on heat-shock response and "diurnal bias" in stress experiments. Heat has significant adverse plant productivity worldwide. Most experiments examining heat are performed during daytime hours, generating a 'diurnal bias' pathways regulatory mechanisms identified. Such bias may confound downstream interpretations limit our understanding full to...
Defining convergent and divergent mechanisms underlying the biogenesis function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, Trans-Golgi Network (TGN) hub where exocytic endocytic pathways converge. To gain knowledge TGN function, we characterized TGNap1, a protein encoded by plant gene unknown conserved with metazoans. We demonstrate that TGNap1 required for homeostasis biosynthetic traffic pathways. also show binds Rab6, YIP4 microtubules....
The evolution of roots and root hairs was a crucial innovation that contributed to the adaptation plants terrestrial environment. Initiation involves transcriptional cues in part determine cell patterning epidermis. Once hair initiation has occurred, elongation takes place. Although many genes have been identified as being involved development, contributors remain uncharacterized. In this study we report on involvement member (here dubbed maMYB) plant-specific R2R3-MYB family transcription...
Cellulose synthases (CESAs) are glycosyltransferases that catalyze formation of cellulose microfibrils in plant cell walls. Seed CESA isoforms cluster six phylogenetic clades, whose non-interchangeable members play distinct roles within synthesis complexes (CSCs). A 'class specific region' (CSR), with higher sequence similarity versus between functional classes, has been suggested to contribute activities or interactions different isoforms. We investigated isoform specificity the moss,...
The β-1,4-glucan chains comprising cellulose are synthesized by synthases in the plasma membranes of diverse organisms including bacteria and plants. Understanding structure–function relationships plant enzymes involved synthesis (CESAs) is important because most abundant component cell wall, a key renewable biomaterial. Here, we explored structure function region encompassing transmembrane helices (TMHs) 5 6 CESA using computational genetic tools. Ab initio prediction revealed novel...
Cellulose synthases (CESAs) synthesize the β-1,4-glucan chains that coalesce to form cellulose microfibrils in plant cell walls. In addition a large cytosolic (catalytic) domain, CESAs have eight predicted transmembrane helices (TMHs). However, analogous structure of BcsA, bacterial CESA, TMH5 CESA may instead be an interfacial helix. This would place conserved FxVTxK motif cytosol where it could function as substrate-gating loop occurs BcsA. To define functional importance region containing...
Membrane-tethered transcription factors (MTTFs) are proteins that targeted to membranes and capable of regulating gene expression. In this way, they physically restrained from entering the nucleus innately dormant. Upon specific signal recognition cues, MTTFs activated through cleavage by a protease releases factor domain into cytosol thus allowing it translocate where can regulate classically thought provide an advantage organism for rapid transduction in response cellular environmental...
RNA-seq analysis has enabled the evaluation of transcriptional changes in many species including nonmodel organisms. However, most only a single reference genome is available and reads from highly divergent varieties are typically aligned to this reference. Here, we quantify impacts choice mapping rice where three high-quality genomes available. We data popular productive variety different found that identification differentially expressed genes differed depending on which was used for...
Abstract Understanding protein structure and function relationships in cellulose synthase (CesA), including divergent isomers, is an important goal. Here, we report results from mutant complementation assays that tested the ability of sequence variants AtCesA7, a secondary wall CesA Arabidopsis thaliana , to rescue collapsed vessels, short stems, low content irx3‐1 AtCesA7 null mutant. We catalytic mutation seven missense or small domain changes near FTVTSK motif, which lies may, analogously...
In many plant species, the time of day at which flowers open to permit pollination is tightly regulated. Proper flower opening, or Time Day Anther Appearance (TAA), may coordinate flowering opening with pollinator activity shift temperature sensitive developmental processes cooler times day. The genetic mechanisms that regulate timing this process in cereal crops are unknown. To address knowledge gap, it necessary establish a monocot model system exhibits variation TAA. Here, we examine...