A5085434127- Plant Molecular Biology Research
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Remote Sensing in Agriculture
- Optical Polarization and Ellipsometry
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Gene Regulatory Network Analysis
- Bioinformatics and Genomic Networks
- Molecular Biology Techniques and Applications
- Plant Reproductive Biology
- Spectroscopy and Chemometric Analyses
- Genomics and Phylogenetic Studies
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Leaf Properties and Growth Measurement
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Pineapple and bromelain studies
- Plant Pathogens and Fungal Diseases
- Genomics and Chromatin Dynamics
- Magnetic and Electromagnetic Effects
- Powdery Mildew Fungal Diseases
- Remote-Sensing Image Classification
- Insect and Arachnid Ecology and Behavior
North Carolina State University
2016-2025
North Central State College
2023
Michigan State University
2009-2015
University of California, San Diego
2010-2015
University of Southern California
2015
University of Chicago
2008
Quinsigamond Community College
2007
Abstract The Arabidopsis thaliana CBF cold response pathway plays a central role in acclimation. It is characterized by rapid induction of genes encoding the CBF1-3 transcription factors, followed expression gene regulon, which imparts freezing tolerance. Our goal was to further understanding cis-acting elements and trans-acting factors involved CBF2. We identified seven conserved DNA motifs (CM), CM1 7, that are present promoters CBF2 another rapidly cold-induced factor, ZAT12. results...
The first described feedback loop of the Arabidopsis circadian clock is based on reciprocal regulation between TIMING OF CAB EXPRESSION 1 (TOC1) and CIRCADIAN CLOCK-ASSOCIATED (CCA1)/LATE ELONGATED HYPOCOTYL (LHY). CCA1 LHY are Myb transcription factors that bind directly to TOC1 promoter negatively regulate its expression. Conversely, activity has remained less well characterized. Genetic data support necessary for reactivation / , but there little description biochemical function. Here we...
Exposure of Arabidopsis thaliana plants to low non-freezing temperatures results in an increase freezing tolerance that involves action the C-repeat binding factor (CBF) regulatory pathway. CBF1, CBF2 and CBF3, which are rapidly induced response temperature, encode closely related AP2/ERF DNA-binding proteins recognize (CRT)/dehydration-responsive element (DRE) DNA present promoters CBF-regulated genes. The CBF transcription factors alter expression more than 100 genes, known as regulon,...
Organisms have evolved endogenous biological clocks as internal timekeepers to coordinate metabolic processes with the external environment. Here, we seek understand mechanism of synchrony between oscillator and products metabolism known Reactive Oxygen Species (ROS) in Arabidopsis thaliana. ROS-responsive genes exhibit a time-of-day-specific phase expression under diurnal circadian conditions, implying role clock transcriptional regulation these genes. Hydrogen peroxide production...
Significance The circadian clock, an endogenous time-keeping mechanism common to most species, allows organisms coordinate biological processes with specific times of day. In plants, the role clock extends almost every aspect growth and development, including responses biotic abiotic stresses. core molecular components circuits have been well studied in model organism Arabidopsis thaliana ; however, how this connects clock-controlled outputs remains poorly understood. Here, we performed a...
Extensive transcriptional networks play major roles in cellular and organismal functions. Transcript levels are part determined by the combinatorial overlapping functions of multiple transcription factors (TFs) bound to gene promoters. Thus, TF-promoter interactions provide basic molecular wiring regulatory networks. In plants, discovery functional TFs is limited an increased complexity network circuitry due a significant expansion TF families. Here, we present construction comprehensive...
Solanum commersonii and tuberosum are closely related plant species that differ in their abilities to cold acclimate; whereas S. increases freezing tolerance response low temperature, does not. In Arabidopsis thaliana, cold-regulated genes have been shown contribute tolerance, including those comprise the CBF regulon, controlled by transcription factors. The temperature transcriptomes regulons of were therefore compared determine whether there might be differences ability acclimate. results...
Using existing protocols, RNA extracted from seeds rich in starch often results poor quality RNA, making it inappropriate for downstream applications. Though some methods are proposed extracting plant tissue and other polysaccharides, they invariably yield less RNA. In order to obtain high tissues including roots a modified SDS-LiCl method was compared with methods, TRIZOL kit (Invitrogen), Plant RNeasy mini (Qiagen), Furtado (2014) method, CTAB-LiCl method. Modifications the extraction...
Abstract Organic photodetectors (OPDs) exhibit performance on par with inorganic detectors (e.g., Si) but can be ultrathin, ultra‐lightweight, flexible, and mechanically resilient, opening up opportunities for novel applications including optical sensors continuous human plant health monitoring. Here, a high‐performance flexible self‐powered OPD designed on‐plant sensing is developed. The employs an electrode consisting of Ag nanowires (NWs) embedded in UV‐curable resin to achieve thin form...
Introduction Understanding how plants adapt to the space environment is essential, as will be a valuable component of long duration missions. Several spaceflight experiments have focused on transcriptional profiling means understanding plant adaptation microgravity. However, there limited overlap between results from different experiments. Differences in experimental conditions and hardware make it difficult find consistent response across distinguish primary effects microgravity other...
Significance The effects of warmer nighttime temperatures (WNT) on crops are one poorly understood dimension climate change. WNT result from the asymmetrical increase in versus daytime temperatures. In rice, reduce grain yield and quality. amplitude daily temperature cycles plants use to set their circadian clock. Therefore, we examined how affect timing molecular activities. field-grown plants, alter pattern transcriptome. Genes with strong rhythmic expression those under control affected...
Background: The growing demand for rare earth elements (REEs), particularly dysprosium (Dy), in part driven by clean energy technologies, underscores the need sustainable extraction methods. Recovery of Dy, from geographically distributed waste sources is challenging. This gap positions phytomining, a technique using plants to accumulate metals, as promising alternative. However, plant species differ their ability metals high concentrations, necessitating efficient screening In this study,...
The circadian clock controls many molecular activities, impacting experimental interpretation. We quantify the genome-wide effects of time-of-day on heat-shock response and "diurnal bias" in stress experiments. Heat has significant adverse plant productivity worldwide. Most experiments examining heat are performed during daytime hours, generating a 'diurnal bias' pathways regulatory mechanisms identified. Such bias may confound downstream interpretations limit our understanding full to...
Sorghum is often exposed to suboptimal low temperature stress under field conditions, particularly at the seedling establishment stage. Enhancing chilling tolerance will facilitate earlier planting and so minimize negative impacts of other stresses experienced later growth stages. Genome-wide association mapping was performed on a sorghum panel grown control (30/20 °C; day/night) (20/10 °C) conditions. Genomic regions chromosome 7, controlling emergence index (root shoot) vigor, were...
SUMMARY RNA‐Sequencing is widely used to investigate changes in gene expression at the transcription level plants. Most plant RNA‐Seq analysis pipelines base normalization approaches on assumption that total transcript levels do not vary between samples. However, this has been demonstrated. In fact, many common experimental treatments and genetic alterations affect efficiency or RNA stability, resulting unequal abundance. The addition of synthetic controls a simple correction for variation...
Abstract A key remit of the NSF ‐funded “Arabidopsis Research and Training for 21 st Century” ( ART ‐21) Coordination Network has been to convene a series workshops with community members explore issues concerning research training in plant biology, including role that using Arabidopsis thaliana can play addressing those issues. first workshop focused on needs bioinformatic computational approaches biology was held 2016, recommendations from have published (Friesner et al., Plant Physiology...
Abstract High night temperatures (HNT) are shown to significantly reduce rice ( Oryza sativa L.) yield and quality. A better understanding of the genetic architecture HNT tolerance will help breeders develop varieties adapted future warmer climates. In this study, a diverse indica panel displayed wide range phenotypic variability in quality traits under control (24 °C) higher (29 temperatures. Genome-wide association analysis revealed 38 loci associated across treatments (18 for 20 HNT)....
Automation of plant phenotyping using data from high-dimensional imaging sensors is on the forefront agricultural research for its potential to improve seasonal yield by monitoring crop health and accelerating breeding programs. A common challenge when capturing images in field relates spectral reflection sunlight (glare) leaves that, at certain solar incidences sensor viewing angles, presents unwanted signals. The presented here involves convergence 2 parallel projects develop a facile...
Organisms respond to changes in their environment through transcriptional regulatory networks (TRNs). The hierarchy of these can be inferred from expression data. Computational approaches identify TRNs applied any species where quality RNA acquired, However, ChIP-Seq and similar validation methods are challenging employ non-model species. Improving the accuracy computational inference significantly reduce cost time subsequent experiments. We have developed ExRANGES, an approach that improves...