A5084583493- Plant Disease Resistance and Genetics
- Weed Control and Herbicide Applications
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Research in Cotton Cultivation
- Pesticide and Herbicide Environmental Studies
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Plant Parasitism and Resistance
- Nanofabrication and Lithography Techniques
- RNA and protein synthesis mechanisms
- Genetic and Environmental Crop Studies
- Food Quality and Safety Studies
- Silicon Effects in Agriculture
- Plant Micronutrient Interactions and Effects
- Soybean genetics and cultivation
- Plant nutrient uptake and metabolism
- RNA Research and Splicing
- Legume Nitrogen Fixing Symbiosis
- Agricultural Productivity and Crop Improvement
- Enzyme-mediated dye degradation
Bayer (United States)
2019-2022
United Soybean Board
2019
Monsanto (United States)
2015-2018
Michigan State University
1991-1994
University of Georgia
1985-1992
The maize regulatory protein Opaque-2 (O2) localizes to the nucleus in both and tobacco cells. Here we show that in-frame carboxy- amino-terminal fusions of O2 reporter beta-glucuronidase (GUS) were sufficient direct GUS transgenic plants transiently transformed onion Two independent regions containing 135 149 amino acids identified able redirect systems. A quantitative biochemical analysis nuclei isolated from revealed second region was more efficient than first one. precise location...
The molecular basis for the low level expression of three alleles maize waxy (Wx) gene has been described. Each allele contains a retrotransposon in intron sequences. These insertions represent previously undescribed elements, and their association with wx indicates that elements are important agents spontaneous mutation maize. For each allele, element sequences spliced from pre-mRNA surrounding even though increase length by approximately 40- to 60-fold. In addition, despite differences...
More than 40 mutant alleles of the waxy (Wx) locus maize are available for molecular analysis. Previous studies have examined nature phenotypically unstable Wx caused by insertion transposable activator (Ac) and dissociation (Ds) elements. In this study we used Southern blot analysis to characterize in 22 strains harboring wx with stable phenotypes. Of these mutations, 17 spontaneous origin, 4 were induced gamma rays, 1 was ethyl methanesulfonate. alleles, find that 13 either insertions or...
In some instances, insertion of maize transposable elements into exons does not result in the total loss enzymatic activity. other messenger RNAs wild-type size are encoded by genes known to contain element Dissociation (Ds) exons. To understand how Ds is processed from RNA, a study was made transcripts two alleles waxy (wx) gene containing insertions exon sequences. The analysis carried out strains where could excise wx gene. Despite 4.3- and 1.5- elements, predominant these were wild type...
Protein targeting to the nucleus has been studied extensively in animal and yeast systems; however, nothing is known about nuclear plants. The Opaque-2 (O2) gene produces a regulatory protein that responsible for inducing transcription of alpha-zein class storage proteins maize kernels. cloned O2 encodes contains leucine zipper DNA binding domain can interact with zein promoters. We have used immunolocalization show present nuclei endosperm tissues produce alpha-zeins. In addition, neither...
For organisms to grow and develop, transcriptional regulatory proteins must localize the nucleus. This movement is mediated by nuclear localization sequences (NLSs) which consist of short stretches basic amino acids that are part structure mature proteins. Two NLSs have been previously identified in maize protein Opaque2 (O2), a member basic‐domain, leucine‐zipper (bZIP) class In this report, it has determined both these necessary for import O2, focus on functional analysis more efficient,...
Abstract A key remit of the NSF ‐funded “Arabidopsis Research and Training for 21 st Century” ( ART ‐21) Coordination Network has been to convene a series workshops with community members explore issues concerning research training in plant biology, including role that using Arabidopsis thaliana can play addressing those issues. first workshop focused on needs bioinformatic computational approaches biology was held 2016, recommendations from have published (Friesner et al., Plant Physiology...
Abstract BACKGROUND Herbicide‐resistant weeds pose a challenge to agriculture and food production. New herbicide tolerance traits in crops will provide farmers with more options effectively manage weeds. Mesotrione, selective pre‐ post‐emergent triketone used corn production, controls broadleaf some annual grass via hydroxyphenylpyruvate dioxygenase (HPPD) inhibition. Recently, the rice HIS1 gene, responsible for native benzobicyclon, was identified. Expression of also confers modest level...
Abstract BACKGROUND Protoporphyrinogen IX oxidase (PPO)‐inhibiting herbicides act by inhibiting a key enzyme in the heme and chlorophyll biosynthetic pathways plants. This enzyme, PPO is conserved across plant species. However, some microbes are known to utilize unique family of enzymes, HemG family. carries out same enzymatic step as but does not share sequence homology with enzymes. RESULTS Bioinformatic analysis was used identify putative variants from microbial sources. A subset these...
Effective management of weedy species in agricultural fields is essential for maintaining favorable growing conditions and crop yields. The introduction genetically modified crops containing herbicide tolerance traits has been a successful additional tool available to farmers better control weeds. However, weed resistance challenges present need trait options.To help meet this challenge, new that provides an aryloxyphenoxypropionate (FOP) members the synthetic auxin family, such as...
Hybrid crops produce higher yields than their inbred parents due to heterosis. For high purity of hybrid seeds, it is critical eliminate self-pollination. Manual or mechanical removal male parts (such as detasseling in maize) labor-intensive, fuel and time-consuming, can cause physical damage female plants, resulting significant seed yield reductions. Many male-sterility systems either require a maintainer for male-sterile line propagation are often affected by environmental factors....