A5045564601- Metabolism and Genetic Disorders
- Osteoarthritis Treatment and Mechanisms
- Lysosomal Storage Disorders Research
- Connective tissue disorders research
- Amino Acid Enzymes and Metabolism
- TGF-β signaling in diseases
- Neonatal Health and Biochemistry
- Silk-based biomaterials and applications
- Renal function and acid-base balance
- Bone Metabolism and Diseases
- Trypanosoma species research and implications
- Diet and metabolism studies
- Molecular Biology Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Biochemical and Molecular Research
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Planarian Biology and Electrostimulation
- Genomics and Rare Diseases
- Mitochondrial Function and Pathology
- Calcium signaling and nucleotide metabolism
- Protease and Inhibitor Mechanisms
- Infant Nutrition and Health
- Metabolomics and Mass Spectrometry Studies
- Pancreatitis Pathology and Treatment
Pontificia Universidad Javeriana
2015-2024
Hospital Universitario San Ignacio
2020
Universidad del Rosario
2020
Icahn School of Medicine at Mount Sinai
2016
Military University Nueva Granada
2015
Background We previously demonstrated the therapeutic benefits of pentosan polysulfate (PPS) in a rat model mucopolysaccharidosis (MPS) type VI. Reduction inflammation, reduction glycosaminoglycan (GAG) storage, and improvement skeletal phenotype were shown. Herein, we evaluate long-term safety effects PPS large animal different MPS type, I dogs. focused on arterial since this is one most consistent clinically significant features model. Methodology/Principal Findings dogs treated with daily...
During embryological limb formation mesenchymal cells condense and differentiate into chondrocytes, in a process known as chondrogenesis. These chondrocytes synthesize glycosaminoglycans (GAGs), thus playing an important role this process. A simplified system vitro chondrogenesis, using adult stromal (MSCs) has been demonstrated. This differentiation potential is usually assessed by histological staining. <strong>Objective</strong>. Establishment of semi-automatic grading for...
Abstract Cell membrane is a lipid bilayer that allows the flow of ions through their ionic pumping proteins. The can be stimulated with external stimuli to activate specific signaling pathways intracellularly. Although studies have applied electric and magnetic modify cell function, parameters stimulate are unknown. Accordingly, computational model simulate effect fields on was developed. Cells were from 45 × 10 3 V/m 12.6 5 2 mT, at frequencies 60 kHz, MHz, 1 GHz. Results showed tend...
Heart transplant is a therapeutic option in the treatment of chagasic cardiomyopathy. For early detection Chagas reactivation cases, use PCR tests using endomyocardial biopsies has been proposed. Development an animal model will be first step evaluating applicability this approach.PCR based on TcH2AF-R and S35-S36 primers were evaluated for T. cruzi heart tissue mice experimentally infected with parasite.Two groups ICR 15 10 individuals by intraperitoneal injection 0.3 ml PBS containing 1 x...
Magnetic fields (MFs) have been used as an external stimulus to increase cell proliferation in chondrocytes and extracellular matrix (ECM) synthesis of articular cartilage. However, previously published studies not shown that MFs are homogeneous through culture systems. In addition, variables such stimulation times MF intensities standardized obtain the best cellular proliferative rate or molecular ECM. this work, a device, which produces stimulate surfaces was designed manufactured using...
Classical galactosemia is caused by the genetic deficiency of galactose-1-phosphate-urydyl-transferase resulting in clinical symptoms development during first weeks life including jaundice, hypotonia, lethargy, emesis, hepatomegaly, among others. Currently, dietary restriction galactose considered standard for classical management. For several years, severe was necessary, implying not only dairy products, but also fruits, vegetables, legumes, and viscera. Such management failed to improve or...
Articular cartilage is characterized by low cell density of only one type, chondrocytes, and has limited self-healing properties. When articular affected traumatic injuries, a therapeutic strategy such as autologous chondrocyte implantation usually proposed for its treatment. This approach requires in vitro expansion to yield high number transplantation. To improve the efficiency this procedure, it necessary assess dynamics migration, proliferation death during culture. Computational models...
Abstract In mammals, long bones are formed by ossification of a cartilaginous mould during early stages development, through the formation structures called primary centre, secondary centres (SOCs) and physeal cartilages (PCs). The PC is responsible for bone growth. morphology SOCs varies different femoral this respect, several details involving process murine development lacking. present study, morphological characterization femur from embryonic period to adulthood in mice was studied using...
The utility of low-resolution 1H-NMR analysis for the identification biomarkers provided evidence rapid biochemical diagnoses organic acidemia and aminoacidopathy. 1H-NMR, with a sensitivity expected field strength 400 MHz at 64 scans was used to establish metabolomic urine sample profiles an infant population diagnosed small molecule Inborn Errors Metabolism (smIEM) compared unaffected individuals. A qualitative differentiation spectral samples obtained from individuals affected by...
Mucopolysaccharidoses (MPS) are a group of inherited metabolic diseases caused by impaired function or absence lysosomal enzymes involved in degradation glycosaminoglycans. Clinically, MPS skeletal dysplasias, characterized cartilage abnormalities and disturbances the process endochondral ossification. Histologic growth have been reported at advanced stages disease, but information regarding plate pathology progression either humans animal models, as well its pathophysiology, is...
The growth plate is a cartilaginous structure located in the metaphysis of long bones, characterized histologically by its stratification and columnar arrangement. It responsible for assuring longitudinal growth. Evaluation histological characteristics has been traditionally performed using qualitative observation; however, some quantitative approaches have reported complex techniques. Here, we propose simple images based analysis order to evaluate objectively arrangement within plate. For...
During skeleton formation, mesenchymal cells condense and differentiate into chondrocytes in a process known as chondrogenesis. Glycosaminoglycans (GAGs), main components of aggrecan the extracellular matrix (ECM), have an important role this process. An vitro simplified system has been devised to study chondrogenesis using progenitor cells. Although capacity stromal chondrogenic lineage is well established, there lack knowledge with respect lysosomal enzyme activity during To further...