Previous studies have demonstrated that insulin-like growth factor-I (IGF-I) increases elastin gene transcription in aortic smooth muscle cells and this up-regulation is accompanied by a loss of protein binding to the proximal promoter. Sp1 has been identified as one factors whose lost, present study we show Sp3 also abrogated IGF-I, but selected manner. In functional analyses using Drosophila SL-2 cells, expression can drive from promoter, while co-expression results repression activity....

The meniscus is divided into three zones according to its vascularity: an external vascularized red-red zone mainly comprising collagen I, a red-white interphase collagens I and II, internal white-white rich in II. Known scaffolds used treat meniscal injuries do not reflect the chemical composition of vascular areas meniscus. Therefore, this study, four composite zonal (named A, B, C, D) were developed characterized; exhibited main components (collagen I), (collagens I/II), II) Noncomposite...

This work assessed wound healing response in rabbit oral lesions grafted with autologous artificial connective tissue or acellular collagen scaffolds. Autologous (AACT) was produced using fibroblasts and I Before implantation, AACT grafts were assayed to demonstrate the presence of extracellular matrix components, as well expression characteristic genes secretion chemokines, cytokines, growth factors. tested rabbits from which obtained, whereas type scaffolds (CS) evaluated a separate group...

The pharmacologic activity of compounds isolated from Physalis peruviana has been demonstrated. use this fruit juice for treating pterygium reported in Colombian traditional medicine. However, studies demonstrating the juice's when used disease have not published to date. In present study anti-inflammatory and cytostatic activities P. a rabbit eye inflammatory model were investigated.A novel inflammation was developed studying (based on an adaptation Draize test). Cytostatic evaluated by...

Previously, we found that oral autologous artificial connective tissue (AACT) had a different protein secretion profile to of clot-embedded AACT. Other mucosa substitutes, having cell types and scaffolds, dissimilar profiles proteins (including for AACT) influence healing outcome; thus, ascertain the factors secreted by whether they are influenced their microstructure might help in understanding bioactivity. An important component is fiber orientation scaffold used manufacturing it. This...

Previous studies demonstrated that basic fibroblast growth factor (bFGF) decreases elastin gene transcription in pulmonary fibroblasts. In this study we pursue the identification of element and trans-acting factors responsible. Gel shift analyses show bFGF increases protein binding to a sequence located at −564 −558 base pairs (bp), which possesses homology both AP1 cAMP-response consensus elements yet displays unique affinity for heterodimer binding. Site-directed mutation −564- −558-bp...

Although many bone substitutes have been designed and produced, the development of tissue engineering products that mimic microstructural characteristics native remains challenging. It has shown pore orientation within collagen scaffolds influences matrix formation by endochondral route. In addition, unidirectional can limit growth blood vessels. However, a comparison between amount be formed in with different orientations addition to analyzing effect loading osteogenic proangiogenic factors...

Several studies have evaluated proteins secreted by fibroblasts comprising skin substitutes, finding that they are in combinations and concentrations promote wound healing. However, assessment of oral forming a part substitutes is scarce. In our previous work, collagen type-I scaffolds (CSs) autologous artificial connective tissue (AACT) were produced implanted rabbit lesions, evidencing AACT outperforms CS. The present work determined the factor profile time grafting as well embedded clot....

Abstract In mammals, long bones are formed by ossification of a cartilaginous mould during early stages development, through the formation structures called primary centre, secondary centres (SOCs) and physeal cartilages (PCs). The PC is responsible for bone growth. morphology SOCs varies different femoral this respect, several details involving process murine development lacking. present study, morphological characterization femur from embryonic period to adulthood in mice was studied using...

Studies have been conducted on the pathogenicity of periodontopathogens in cultures endothelial cells two-dimensional (2D) polystyrene surfaces, where monolayer formed is not exposed to proteins subendothelial matrix. In this work, we developed a culture system by seeding human coronary artery (HCAECs) onto three-dimensional (3D) scaffolds collagen type I, protein. The inflammatory responses HCAEC monolayers, either 3D or directly 2D plate, lipopolysaccharide (LPS) from Aggregatibacter...

Analysis of factors that play a role on the healing process in exudates from skin wounds might shed light effect grafted artificial tissue has wound regeneration and repair. The first objective this work was to standardize an optic surface plasmon resonance method based self-assembled monolayers quantify mediator (angiopoietin-2, epidermal growth factor, tumour necrosis factor-α, transforming factor-β1, vascular endothelial factor) exudates. Optimal conditions for self-assembling alkanethiol...

Mesenchymal stem cells (MSCs) differentiate to that are part of tissues, such as bone and cartilage, both during ontogeny for the repair/regeneration renewal tissues postnatal life. Accordingly, MSCs can be a powerful tool regenerative medicine tissue engineering interventions in musculoskeletal deficiencies. found marrow connective exposed biochemical biophysical factors modulate their biological behavior. Given have been widely studied, research on effect stimuli differentiation is scarce....

Abstract The role of periodontopathogens in inflammatory endothelial dysfunction is not known. This study characterizes a three‐dimensional model with human coronary artery cells on (HCAEC‐3D) type I collagen scaffolds to evaluate whether infection Aggregatibacter actinomycetemcomitans induces proinflammatory response associated atherosclerosis. HCAEC‐3D culture was physicochemically characterized regard biocompatibility and barrier function. Then, the infected A. strain ATCC 29522 at...