A5020191271- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
Max Planck Institute of Biophysics
2022-2023
Chinese University of Hong Kong
2019
Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization chromosomes1. Whereas cohesin and condensin organize by extrusion DNA loops, molecular functions third eukaryotic SMC complex, Smc5/6, remain largely unknown2. Using single-molecule imaging, we show that Smc5/6 forms loops extrusion. Upon ATP hydrolysis, reels symmetrically into at a force-dependent rate one kilobase pair per second. extrudes in form dimers, whereas monomeric...
Macroautophagy/autophagy is an essential process for the maintenance of cellular homeostasis by recycling macromolecules under normal and stress conditions. ATG9 (autophagy related 9) only integral membrane protein in autophagy core machinery has a central role mediating autophagosome formation. In cells, exists on mobile vesicles that traffic to growing phagophore, providing source formation autophagosomes. Here we report three-dimensional structure from Arabidopsis thaliana at 7.8 Å...
Abstract Structural Maintenance of Chromosomes (SMC) protein complexes are essential for the spatial organization chromosomes. While cohesin and condensin organize chromosomes by extruding DNA loops, molecular functions third eukaryotic SMC complex, Smc5/6, remain largely unknown. Using single-molecule imaging, we reveal that Smc5/6 forms loops extrusion. Upon ATP-hydrolysis, symmetrically reels into at a force-dependent rate 1 kilobase pairs per second. extrudes in form dimer, while...
Abstract Structural Maintenance of Chromosomes (SMC) protein complexes are essential for the spatial organization chromosomes. While cohesin and condensin organize chromosomes by extruding DNA loops, molecular functions third eukaryotic SMC complex, Smc5/6, remain largely unknown. Using single-molecule imaging, we reveal that Smc5/6 forms loops extrusion. Upon ATP-hydrolysis, symmetrically reels into at a force-dependent rate 1 kilobase pairs per second. extrudes in form dimer, while...