A5035667960- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- Genomic variations and chromosomal abnormalities
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
Karolinska Institutet
2022-2024
Integrated Cardio Metabolic Centre
2022-2023
Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization chromosomes1. Whereas cohesin and condensin organize by extrusion DNA loops, molecular functions third eukaryotic SMC complex, Smc5/6, remain largely unknown2. Using single-molecule imaging, we show that Smc5/6 forms loops extrusion. Upon ATP hydrolysis, reels symmetrically into at a force-dependent rate one kilobase pair per second. extrudes in form dimers, whereas monomeric...
The structural maintenance of chromosomes (SMC) protein complexes—cohesin, condensin, and the Smc5/6 complex (Smc5/6)—are essential for chromosome function. At molecular level, these complexes fold DNA by loop extrusion. Accordingly, cohesin creates loops in interphase, condensin compacts mitotic chromosomes. However, role Smc5/6's recently discovered extrusion activity is unknown. Here, we uncover that associates with transcription-induced positively supercoiled at cohesin-dependent...
Abstract Structural Maintenance of Chromosomes (SMC) protein complexes are essential for the spatial organization chromosomes. While cohesin and condensin organize chromosomes by extruding DNA loops, molecular functions third eukaryotic SMC complex, Smc5/6, remain largely unknown. Using single-molecule imaging, we reveal that Smc5/6 forms loops extrusion. Upon ATP-hydrolysis, symmetrically reels into at a force-dependent rate 1 kilobase pairs per second. extrudes in form dimer, while...
SUMMARY The Structural Maintenance of Chromosome (SMC) protein complexes cohesin, condensin and the Smc5/6 complex (Smc5/6) are essential for chromosome function. At molecular level, these fold DNA by loop extrusion. Accordingly, cohesin creates loops in interphase, compacts mitotic chromosomes. However, role Smc5/6’s recently discovered extrusion activity is unknown. Here, we uncover that associates with transcription-induced positively supercoiled chromosomal at cohesin-dependent...
Abstract Structural Maintenance of Chromosomes (SMC) protein complexes are essential for the spatial organization chromosomes. While cohesin and condensin organize chromosomes by extruding DNA loops, molecular functions third eukaryotic SMC complex, Smc5/6, remain largely unknown. Using single-molecule imaging, we reveal that Smc5/6 forms loops extrusion. Upon ATP-hydrolysis, symmetrically reels into at a force-dependent rate 1 kilobase pairs per second. extrudes in form dimer, while...