A5021518824- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Toxic Organic Pollutants Impact
- Insect Utilization and Effects
- Advanced Chemical Sensor Technologies
- Insect and Pesticide Research
- Analytical Chemistry and Chromatography
- Pesticide Residue Analysis and Safety
- Effects and risks of endocrine disrupting chemicals
- Genomics and Chromatin Dynamics
- Mass Spectrometry Techniques and Applications
- Genomics and Phylogenetic Studies
- Environmental Toxicology and Ecotoxicology
- Biofuel production and bioconversion
- Chromosomal and Genetic Variations
- Isotope Analysis in Ecology
- Genetic Mapping and Diversity in Plants and Animals
- Chemical Analysis and Environmental Impact
- Eicosanoids and Hypertension Pharmacology
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Enzyme Production and Characterization
- Chemical Thermodynamics and Molecular Structure
- Pharmacogenetics and Drug Metabolism
- Transgenic Plants and Applications
European Molecular Biology Laboratory
2022-2025
European Molecular Biology Laboratory
2022
European Bioinformatics Institute
2021
University of Liège
2007-2016
ETH Zurich
2014
National Bank of Belgium
2003
Abstract The current human reference genome, GRCh38, represents over 20 years of effort to generate a high-quality assembly, which has benefitted society 1,2 . However, it still many gaps and errors, does not represent biological genome as is blend multiple individuals 3,4 Recently, telomere-to-telomere reference, CHM13, was generated with the latest long-read technologies, but derived from hydatidiform mole cell line nearly homozygous 5 To address these limitations, Human Pangenome...
Structural maintenance of chromosomes (SMC) protein complexes structure genomes by extruding DNA loops, but the molecular mechanism that underlies their activity has remained unknown. We show active condensin complex entraps bases a loop transiently in two separate chambers. Single-molecule imaging and cryo-electron microscopy suggest putative power-stroke movement at first chamber feeds into SMC-kleisin ring upon adenosine triphosphate binding, whereas second holds on upstream same double...
Soon after death, the decay process of mammalian soft tissues begins and leads to release cadaveric volatile compounds in surrounding environment. The study postmortem decomposition products is an emerging field forensic science. However, a better knowledge smell death its constituents may have many applications sciences. Domestic pigs are most widely used human body analogues experiments, mainly due ethical restrictions. Indeed, trials on corpses restricted countries worldwide. This article...
In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. yeast, partitioning of such circles the mother cell at mitosis ensures their loss from population promotes replicative ageing. Nevertheless, mechanisms are debated. this study, we show that SAGA complex mediates interaction non-chromosomal with nuclear pore complexes (NPCs) and thereby confinement in cell. Reciprocally, causes retention accumulation NPCs, which affects...
There has been tremendous progress in phased genome assembly production by combining long-read data with parental information or linked-read data. Nevertheless, a typical generated trio-hifiasm still generates more than 140 gaps. We perform detailed analysis of gaps, breaks, and misorientations from 182 haploid assemblies obtained diversity panel 77 unique human samples. Although trio-based approaches using HiFi are the current gold standard, chromosome-wide phasing accuracy is comparable...
The AAA ATPase p97/VCP forms complexes with different adapters to fulfill distinct cellular functions. We analyzed the structural organization of Ufd1-Npl4 adapter complex and its interaction p97 compared it another adapter, p47. found that binary a heterodimer cooperatively interacts via bipartite binding mechanism. Binding site 1 (BS1) is short hydrophobic stretch in C-terminal domain Ufd1. second located at N terminus Npl4 activated upon Ufd1 Npl4. It consists about 80 amino acids are...
The functional impact and cellular context of mosaic structural variants (mSVs) in normal tissues is understudied. Utilizing Strand-seq, we sequenced 1,133 single-cell genomes from 19 human donors increasing age, discovered the heterogeneous mSV landscapes hematopoietic stem progenitor cells. While mSVs are continuously acquired throughout life, expanded subclones our cohort confined to individuals >60. Cells already harboring more likely acquire additional somatic variants, including...
The most common chemotherapeutics induce DNA damage to eradicate cancer cells, yet defective repair can propagate mutations, instigating therapy resistance and secondary malignancies. Structural variants (SVs), arising from copy-number-imbalanced -balanced rearrangements, are a major driver of tumor evolution, understudied posttherapy. Here, we adapted single-cell template-strand sequencing (Strand-seq) HER2+ breast model investigate the formation doxorubicin-induced de novo SVs. We coupled...
Abstract The current human reference genome, GRCh38, represents over 20 years of effort to generate a high-quality assembly, which has greatly benefited society 1, 2 . However, it still many gaps and errors, does not represent biological genome since is blend multiple individuals 3, 4 Recently, telomere-to-telomere CHM13, was generated with the latest long-read technologies, but derived from hydatidiform mole cell line duplicate thus nearly homozygous 5 To address these limitations, Human...
Melatonin exerts a variety of physiologic activities that are mainly relayed through the melatonin receptors MT1 and MT2 Low expressions these in tissues have led to widespread experimental use agonist 2-[(125)I]-iodomelatonin as substitute for melatonin. We describe three iodinated ligands: 2-(2-[(2-iodo-4,5-dimethoxyphenyl)methyl]-4,5-dimethoxy phenyl) (DIV880) (2-iodo-N-2-[5-methoxy-2-(naphthalen-1-yl)-1H-pyrrolo[3,2-b]pyridine-3-yl])acetamide (S70254), which specific ligands at...
Hindgut homogenates of the termite Reticulitermes santonensis were incubated with carboxymethyl cellulose (CMC), crystalline celluloses or xylan substrates. Hydrolysates analyzed matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectrometry (MALDI-TOF MS). The method was first set up using acid hydrolysis analysis characterize non-enzymatic profiles. Commercial enzymes Trichoderma reesei T. longibrachiatum also tested validate enzymatic analysis. For CMC hydrolysis,...
More detailed information regarding the early mucosal events that lead to intestinal metaplasia would be very beneficial for understanding pathogenesis of Barrett's esophagus (BE). Gastroesophageal reflux and duodenogastroesophageal play a major role in esophagus. The aim this study was investigate prevalence newly developed BE patients who had previously undergone subtotal esophagectomy - clinical condition characterized by absence lower esophageal sphincter massive gastroesophageal...
Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOFMS) has been used to analyze complex mixtures of volatile organic compounds (VOCs) produced during cadaveric decomposition processes. The use specific spectrometric scripting approaches permitted easily identify gravesoils from control soils. high peak capacity the system, as well development a data processing approach allowed isolation and identification several hundreds analytes. When coupled...
Abstract SMC protein complexes structure genomes by extruding DNA loops, but the molecular mechanism that underlies their activity has remained unknown. We show active condensin complex entraps bases of a loop in two separate chambers. Single-molecule and cryo-electron microscopy provide evidence for power-stroke movement at first chamber feeds into SMC-kleisin ring upon ATP binding, while second holds on upstream same double helix. Unlocking strict separation ‘motor’ ‘anchor’ chambers turns...