A5051500900- Mycobacterium research and diagnosis
- Microfluidic and Bio-sensing Technologies
- Infectious Diseases and Mycology
- Tuberculosis Research and Epidemiology
- Microfluidic and Capillary Electrophoresis Applications
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Phagocytosis and Immune Regulation
- Veterinary medicine and infectious diseases
- Malaria Research and Control
- Lipid Membrane Structure and Behavior
- Ginseng Biological Effects and Applications
- Plant Pathogenic Bacteria Studies
- Electrowetting and Microfluidic Technologies
- Immune Cell Function and Interaction
- Plant tissue culture and regeneration
- Glycosylation and Glycoproteins Research
- Nanopore and Nanochannel Transport Studies
- Infectious Diseases and Tuberculosis
- Evolutionary Game Theory and Cooperation
- Mosquito-borne diseases and control
- Complement system in diseases
- Insect Resistance and Genetics
Knoxville College
2024
University of Tennessee at Knoxville
2015-2024
Yale University
2023
Lincoln Memorial University
2023
Louisiana Department of Wildlife and Fisheries
2006-2022
United States Department of Agriculture
2006-2021
National Institute for Mathematical and Biological Synthesis
2016-2017
Rajasthan University of Veterinary and Animal Sciences
2011
Guru Angad Dev Veterinary and Animal Sciences University
2011
Emory University
2011
This work presents an aptamer-based, highly sensitive and specific sensor for atto- to femtomolar level detection of bisphenol A (BPA). Because its widespread use in numerous products, BPA enters surface water from effluent discharges during manufacture, use, waste landfill sites throughout the world. On-site measurement concentrations is important evaluating compliance with quality standards or environmental risk levels harmful compound environment. The this porous, conducting,...
Phosphatidylserine (PS) is a membrane phospholipid which in intact cells exclusively localized the inner leaflet of lipid bilayer. However, once undergo apoptosis or oxidative stress, PS molecules are exposed on external surface and this contributes to their adherence macrophages endothelial cells. exposure Plasmodium falciparum-infected red was determined by flow cytometry using fluorescein-labeled annexin V, specifically binds PS. Involvement malaria-infected examined vitro cytoadherence...
This work presents an AC electrokinetic impedance sensing method that is capable of detecting specific interactions between macromolecules such as antigen–antibody binding. Serum samples were added to the surface interdigitated electrodes had been coated with bacterial antigens. After applying signal 100 mV at a frequency continuously, electrodes' change was recorded and used determine occurrence level antibody binding antigen. Our theoretical analysis indicated this signal, target will...
Vertebrates are constantly exposed to pathogens, and the adaptive immunity has most likely evolved control clear such infectious agents. CD4(+) T cells major players in immune response pathogens. Following recognition of pathogen-derived antigens naïve differentiate into effectors which then pathogen replication either directly by killing pathogen-infected or assisting with generation cytotoxic lymphocytes (CTLs) pathogen-specific antibodies. Pathogen-specific effector highly heterogeneous...
Johne's disease (JD), a persistent and slow progressing infection of ruminants such as cows sheep, is caused by replicating bacilli Mycobacterium avium subspecies paratuberculosis (MAP) infecting macrophages in the gut. Infected animals initially mount cell-mediated CD4 T cell response against MAP which characterized production interferon (Th1 response). Over time, Th1 diminishes most antibody to antigens becomes dominant (Th2 The switch from Th2 occurs concomitantly with progression...
In the year 2007, a total of 200 faecal samples comprising 100 each from cattle and buffaloes different locations Bikaner, Rajasthan were analyzed to confirm presence gastrointestinal parasitic infection.Twenty four (12.00%) found positive for strongyle eggs.Eleven per cent 13 be helminthosis.The prevalence in varied 9.09 12.50 locations.Prevalence range was slightly higher which ranged between 10.52 14.81.The estimation EPG count Strongyle species 200-1000, with an average...
Johne's disease is an infectious gastrointestinal in ruminants caused by Mycobacterium avium subsp. paratuberculosis that causes diarrhea, emaciation, decreased milk production and eventually death. The transmitted utero via colostrums to calves, fecal-orally all age classes. Financial losses due the are estimated be over $200 million US dairy industry. goal of this study was evaluate cost effectiveness control measures based on diagnosis with a sensitive ELISA, EVELISA. An agent-based,...
Shiga toxin-producing Escherichia coli (STEC) is causing outbreaks worldwide and a rapid detection method urgently needed. Loop-mediated isothermal amplification (LAMP) has attracted attention in the development of pathogen methods; however, current methods for LAMP amplicon suffer some drawbacks. In this study, we designed new by incorporating peroxidase-mimicking G-quadruplex DNAzyme simple colorimetric amplicon. As produces containing two molecules per unit, was termed Dual (cDDLAMP)....
Early apoptotic Jurkat T cells undergo capping of CD43, and its polylactosaminyl saccharide chains serve as ligands for phagocytosis by macrophages. This suggests the presence a polylactosaminoglycan-binding receptor on Here we show that this is nucleolin, multifunctional shuttling protein present in nucleus, cytoplasm, surface some types cells. Nucleolin was detected at macrophages, anti-nucleolin antibody inhibited binding early to Nucleolin-transfected HEK293 expressed nucleolin cell...
ABSTRACT Johne's disease (JD), or paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis , is one of the most widespread and economically important diseases livestock wild ruminants worldwide. Control JD could be accomplished diagnosis good animal husbandry, but this currently not feasible because commercially available diagnostic tests have low sensitivity levels are incapable diagnosing prepatent infections. In study, a highly sensitive subspecies-specific enzyme-linked...
Mycobacterial infections-tuberculosis (TB), bovine tuberculosis (bTB), and Johne's disease (JD)-are major infectious diseases of both human animals. Methods presently in use for diagnosis mycobacterial infections include bacterial culture, nucleic acid amplification, tuberculin skin test, interferon-γ assay, serology. Serological tests have several advantages over other methods, including short turn-around time, relatively simple procedures, low cost. However, current serodiagnostic methods...
Abstract Recent outbreaks of Zika virus have been declared a public health emergency international concern. The diagnosis infection is based on person's recent travel history, symptoms, and laboratory test results. However, the may be delayed because symptoms are often mild nondescript, confirmatory tests relatively time‐consuming expensive. Given lack an effective vaccine against virus, short period viremia, developing rapid sensitive means detecting in serum priority. This work presents...
Effective and efficient animal disease detection control have drawn increasing attention in smart farming recent years. It is crucial to explore how harvest data enable data-driven decision making for rapid diagnosis early treatment of infectious diseases among herds. This article proposes an IoT-based social behavior sensing framework model mastitis propagation infer infection risks dairy cows. To monitor cow behaviors, we deploy portable GPS devices on cows track their movement...
A novel mechanism of phagocytic recognition apoptotic cells was found and characterized. Jurkat incubated with appropriate concentrations etoposide or anti-Fas antibody transiently became susceptible to binding phagocytosis by THP-1 cell-derived macrophages at 2 h. The bound showed no chromatin condensation, but the prevented a caspase inhibitor, indicating that they were recognized an early stage apoptosis. ligands on sialylpolylactosaminyl sugar chains because 1) inhibited oligosaccharide...