A5041754337- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Metabolism, Diabetes, and Cancer
- Microbial Metabolic Engineering and Bioproduction
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Glioma Diagnosis and Treatment
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Diet and metabolism studies
- Biosensors and Analytical Detection
- Plasmonic and Surface Plasmon Research
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Lymphatic System and Diseases
- Nanopore and Nanochannel Transport Studies
- Digestive system and related health
- Lymphatic Disorders and Treatments
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Folate and B Vitamins Research
- Single-cell and spatial transcriptomics
Ludwig Cancer Research
2022
University of Lausanne
2022
Karolinska Institutet
2017-2020
Karolinska University Hospital
2017-2020
Sabancı Üniversitesi
2012-2015
Intestinal lymphatic vessels are essential for dietary lipid absorption and immune cell trafficking. Specialized villus capillaries, lacteals, undergo constant VEGF-C-dependent renewal to maintain their function in a hyperosmolar, inflammatory microenvironment exposed by-products. The mechanisms of lacteal adaptation remain incompletely understood. We integrated new published single-cell RNA-sequencing data profile murine small-intestinal endothelial cells (LECs) identified three distinct...
Modern mass spectrometry-based metabolomics is a key technology for biomedicine, enabling discovery and quantification of wide array biomolecules critical human physiology. Yet, only fraction metabolites have been structurally determined, the majority features in typical data remain unknown. To date, metabolite identification relies largely on comparing MS2 fragmentation patterns against known standards, related compounds or predicted spectra. Here, we propose an orthogonal approach to...
Biological samples such as tissues, blood, or tumors are often complex and harbor heterogeneous populations of cells. Separating out specific cell types subpopulations from mixtures to study their metabolic phenotypes is challenging because experimental procedures for separation may disturb the state To address this issue, we developed a method analysis using stable isotope tracing fluorescence-activated sorting followed by liquid chromatography-high-resolution mass spectrometry. ensure...
Small intestinal villi are structural and functional units present in higher vertebrates uniquely adapted to nutrient absorption. Villus enterocytes organized transcriptional "zones" dedicated specialized tasks such as absorption of specific nutrients. We report that the transcription factor c-MAF is expressed differentiated lower mid-villus a target BMP signaling. Maf inactivation perturbed villus zonation program by increasing carbohydrate-related transcripts while suppressing linked...
Abstract Introduction Choline is an essential human nutrient that particular important for proliferating cells, and altered choline metabolism has been associated with cancer transformation. Yet, the various metabolic fates of in cells have not investigated systematically. Objectives This study aims to map products normal cancerous cells. Methods We performed 13 C-choline tracing followed by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis vitro-transformed...
Proliferating cells must synthesize a wide variety of macromolecules while progressing through the cell cycle, but coordination between cycle progression and cellular metabolism is still poorly understood. To identify metabolic processes that oscillate over we performed comprehensive, non-targeted liquid chromatography-high resolution mass spectrometry (LC-HRMS) based metabolomics HeLa isolated in G1 SG2M phases, capturing thousands diverse metabolite ions. When accounting for increased...
Mammalian cell types exhibit specialized metabolism, and there is ample evidence that various co-existing engage in metabolic cooperation. Moreover, even cultures of a single type may contain cells distinct states, such as resting or cycling cells. Methods for measuring activities subpopulations are valuable tools understanding cellular metabolism. Complex populations most commonly separated using sorter, isolated by this method can be analyzed metabolomics methods. However, problem with...
Vascular Endothelial Growth Factor (VEGF) is a signalling protein known as serum biomarker for number of diseases including cancer. Therefore, it necessary to develop label-free, rapid and sensitive method detecting VEGF in its native forms human serum. In this work, we have demonstrated the proof-of-concept label-free biosensor based on capacitance changes induced by capturing using anti-VEGF aptamers functionalized interdigitated (IDE) capacitor arrays (aptasensors). Limit detection...
Mammalian cell types exhibit specialized metabolism, and there is ample evidence that various co-existing engage in metabolic cooperation. Moreover, even cultures of a single type may contain cells distinct states, such as resting or cycling cells. Methods for measuring activities subpopulations are valuable tools understanding cellular metabolism. Complex populations most commonly separated using sorter, isolated by this method can be analyzed metabolomics methods. However, problem with...
Alterations in both cell cycle regulation and cellular metabolism are associated with cancer transformation, enzymes active the committed phase could represent vulnerabilities of cells. Here, we map metabolic events G1 SG2M phases by combining sorting mass spectrometry-based isotope tracing, revealing hundreds cycle-associated metabolites. In particular, arginine uptake ornithine synthesis was during transformed but not normal An integrative data analysis implicated arginase 2 (ARG2) enzyme...
Abstract Proliferating cells must synthesize a wide variety of macromolecules while progressing through the cell cycle, but coordination between cycle progression and cellular metabolism is still poorly understood. To identify metabolic processes that oscillate over we performed comprehensive, non-targeted liquid chromatography-high resolution mass spectrometry (LC-HRMS) based metabolomics HeLa isolated in G 1 SG 2 M phases, capturing thousands diverse metabolite ions. When accounting for...