A5012923767- Lymphatic System and Diseases
- Genomics and Chromatin Dynamics
- Barrier Structure and Function Studies
- Cancer Cells and Metastasis
- Energy, Environment, Agriculture Analysis
- Plant Pathogenic Bacteria Studies
- Intracerebral and Subarachnoid Hemorrhage Research
- CRISPR and Genetic Engineering
- Sexual function and dysfunction studies
- Lymphatic Disorders and Treatments
- Plant pathogens and resistance mechanisms
- Plant-Microbe Interactions and Immunity
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Neonatal Respiratory Health Research
- Vascular Malformations Diagnosis and Treatment
Uppsala University
2023-2025
Abstract During embryogenesis, endothelial cells (ECs) are generally described to arise from a common pool of progenitors termed angioblasts, which diversify through iterative steps differentiation form functionally distinct subtypes ECs. A key example is the formation lymphatic ECs (LECs), thought largely transdifferentiation venous endothelium. Opposing this model, here we show that initial expansion mammalian LECs primarily driven by in situ mesenchymal and does not require transition an...
Abstract Methods for modifying gene function at high spatiotemporal resolution in mice have revolutionized biomedical research, with Cre-loxP being the most widely used technology. However, technology has several drawbacks, including weak activity, leakiness, toxicity, and low reliability of existing Cre-reporters. This is mainly because different genes flanked by loxP sites (floxed) vary their sensitivity to Cre-mediated recombination. Here, we report generation, validation, utility...
Intestinal lymphatic vessels are essential for dietary lipid absorption and immune cell trafficking. Specialized villus capillaries, lacteals, undergo constant VEGF-C-dependent renewal to maintain their function in a hyperosmolar, inflammatory microenvironment exposed by-products. The mechanisms of lacteal adaptation remain incompletely understood. We integrated new published single-cell RNA-sequencing data profile murine small-intestinal endothelial cells (LECs) identified three distinct...
Abstract Lymphatic capillaries continuously take up interstitial fluid and adapt to resulting changes in vessel calibre 1–3 . The mechanisms by which the permeable monolayer of loosely connected lymphatic endothelial cells (LECs) 4 maintains mechanical stability remain elusive. Here we identify dynamic cytoskeletal regulation LEC shape, induced isotropic stretch, as crucial for integrity function dermal capillaries. We found that oak leaf-shaped LECs showed a spectrum VE-cadherin-based...
Cerebral cavernous malformation (CCM) is a disease characterized by vascular malformations that primarily develop in the brain. These are prone to leak, and their rupture or thrombotic closure can cause life-threatening hemorrhages strokes. Mouse models have been instrumental study disease, but most premature lethality, precluding investigation of mechanisms through intravital microscopy. Current mouse also do not recapitulate human CCM skin lesions. Endothelial-specific deletion Ccm3 via...
Endothelial cells (ECs) of blood and lymphatic vessels have distinct identity markers that define their specialized functions. Recently, hybrid vasculatures with both vessel-specific features been discovered in multiple tissues. Here, we identify the penile cavernous sinusoidal (pc-Ss) as a new vascular bed expressing key EC genes Prox1, Vegfr3 ,and Lyve1 . Using single-cell transcriptome data human corpus cavernosum tissue, found heterogeneity within pc-S endothelia observed transcriptional...
Abstract Endothelial cells (ECs) of blood and lymphatic vessels have distinct identity markers that define their specialized functions. Recently, hybrid vasculatures with both vessel-specific features been discovered in multiple tissues. Here, we identify the penile cavernous sinusoidal (pc-S) vasculature as a new vascular bed expressing key EC genes Prox1, Vegfr3 Lyve1 . Using single cell transcriptome data human corpus cavernosum tissue, found heterogeneity within pc-S endothelia observed...