A5062704410- Cell Adhesion Molecules Research
- Retinal Diseases and Treatments
- Intracerebral and Subarachnoid Hemorrhage Research
- Retinal Development and Disorders
- Barrier Structure and Function Studies
- Kruppel-like factors research
- Apelin-related biomedical research
- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
- Angiogenesis and VEGF in Cancer
- Click Chemistry and Applications
- Galectins and Cancer Biology
- Congenital heart defects research
- Bladder and Urothelial Cancer Treatments
- Chemokine receptors and signaling
- Colorectal Cancer Screening and Detection
- Photochromic and Fluorescence Chemistry
- Vascular Malformations Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- Developmental Biology and Gene Regulation
- Molecular Junctions and Nanostructures
- Esophageal Cancer Research and Treatment
- Nanofabrication and Lithography Techniques
- Lymphatic System and Diseases
University Medical Center Hamburg-Eppendorf
2024-2025
Uppsala University
2021-2025
Universität Hamburg
2024-2025
Max Planck Institute for Molecular Biomedicine
2018-2024
University of Tübingen
2021-2023
International Max Planck Research School for Advanced Methods in Process and Systems Engineering
2021
Max Planck Institute of Molecular Physiology
2017
TU Dortmund University
2010-2017
Leibniz Research Centre for Working Environment and Human Factors
2010
Pericytes and vascular smooth muscle cells, collectively known as mural are recruited through PDGFB (platelet-derived growth factor B)-PDGFRB receptor beta) signaling. MCs essential for integrity, their loss has been associated with numerous diseases. Most of this knowledge is based on studies in which insufficiently or fully absent upon inducible ablation. In contrast, little about the physiological consequences that result from impairment specific MC functions. Here, we characterize role...
The understanding of spatially complex biological systems is greatly aided by the availability high resolution information on their tissue architecture, as provided optical sectioning microscopy like confocal and light sheet microscopy. In addition, genetically encoded fluorescent reporter proteins reveal architecture without need for staining procedures. Owing to opacity caused scattering absorption, in limited thin layers a few micrometers traditionally histological sections. Aiming allow...
Abstract Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane highly dynamic, making it difficult in space and time. In particular, sustained local control of molecular function challenging owing lateral diffusion targeted molecules. Herein we present “molecular activity painting” (MAP), novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The...
The Notch pathway is a major regulator of endothelial transcriptional specification. Targeting the receptors or Delta-like ligand 4 (Dll4) dysregulates angiogenesis. Here, by analyzing single and compound genetic mutants for all signaling members, we find significant differences in way ligands regulate liver vascular homeostasis. Loss caused hypermitogenic cell-cycle arrest senescence. Conversely, Dll4 loss triggered strong Myc-driven switch inducing proliferation tip-cell state. Myc...
Cerebral cavernous malformation (CCM) is a disease characterized by vascular malformations that primarily develop in the brain. These are prone to leak, and their rupture or thrombotic closure can cause life-threatening hemorrhages strokes. Mouse models have been instrumental study disease, but most premature lethality, precluding investigation of mechanisms through intravital microscopy. Current mouse also do not recapitulate human CCM skin lesions. Endothelial-specific deletion Ccm3 via...
Die akute subzelluläre Rekrutierung von Proteinen ist eine leistungsfähige Methode zur Untersuchung biologischer Netzwerke. Hoch-dynamische Signalprozesse an der Plasmamembran können bisher jedoch nur bedingt räumlich und zeitlich untersucht werden. Insbesondere durch laterale Diffusion anhaltende lokale Steuerung molekularer Funktionen schwer zu realisieren. Hier wird die “Molekulare-Aktivitäts-Malerei” (Molecular Activity Painting, MAP) vorgestellt, neuartige Technik, photoaktivierbare...
Background Stroke is a leading global cause of human death and disability, with advanced aging associated elevated incidences stroke. Despite high mortality morbidity stroke, the mechanisms to blood-brain barrier dysfunction development stroke age are poorly understood. In vasculature brain, endothelial cells (ECs) constitute core component provide physical composed tight junctions, adherens basement membrane. Methods Results We show, in mice, incidents intracerebral bleeding increases age....
Serum response factor (SRF) controls the expression of muscle contraction and motility genes in mural cells (MCs) vasculature. In retina, MC-SRF is important for correct angiogenesis during development continuing maintenance vascular tone. The purpose this study was to provide further insights into effects MC SRF deficiency on vasculature function mature retina SrfiMCKO mice that carry a MC-specific deletion Srf. Retinal morphology integrity were analyzed vivo via scanning laser...
<title>Abstract</title> Tyrosine kinase receptors and their cognate ligands are critical regulators of vascular endothelial cell proliferation survival. However, less is known about the ability G protein-coupled receptor (GPCR) superfamily to selectively shape lymphatic vasculature in specific vessel beds or organs. The recently emerging roles chemokine CXCR4 its ligand CXCL12 formation cardiac dermal arterial hierarchic vasculature, tip migration lymph guidance mouse zebrafish prompted us...
Menschen auf der ganzen Welt schnitzen ihre Initialen in Bäume als Zeichen ewiger Liebe. Diese Schnitzereien sind eine Million Mal größer die Malereien lebenden Zellen, Y.-W. Wu, L. Dehmelt und Mitarbeiter ihrer Zuschrift S. 6010 ff. vorstellen. Und auch wenn diese Zellmalereien nicht für Ewigkeit sind, halten sie doch mehrere Stunden – sehr lange Zeit dynamischen Umgebung einer Zelle. Die dienen dem bloßen Vergnügen, sondern werden zur Manipulation von Zellfunktionen genutzt, um damit ein...
Abstract Intracerebral hemorrhage (ICH), a devastating form of stroke, is leading global cause human death and disability. The major risk factors for ICH include increasing age, hypertension cerebral amyloid angiopathy. Despite high mortality morbidity associated with ICH, the mechanisms to blood-brain barrier (BBB) dysfunction age development hemorrhagic stroke poorly understood. In vasculature central nervous system, endothelial cells (ECs) constitute core component BBB provide physical...
Abstract Rationale Pericytes (PCs) and vascular smooth muscle cells (vSMCs), collectively known as mural (MCs), are recruited through PDGFB-PDGFRB signaling. MCs essential for integrity, their loss has been associated with numerous diseases. Most of this knowledge is based on studies in which insufficiently or fully absent upon inducible ablation. In contrast, little about the physiological consequences that result from impairment specific MC functions. Objective Here, we characterize role...