A5003411214- Algal biology and biofuel production
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Photosynthetic Processes and Mechanisms
- Renal and related cancers
- Force Microscopy Techniques and Applications
- Mechanisms of cancer metastasis
- Electrohydrodynamics and Fluid Dynamics
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- MicroRNA in disease regulation
- Cellular Mechanics and Interactions
- Micro and Nano Robotics
- Magnesium Alloys: Properties and Applications
- Marine and coastal ecosystems
- ATP Synthase and ATPases Research
- Metalloenzymes and iron-sulfur proteins
- Microbial Metabolic Engineering and Bioproduction
- Physiological and biochemical adaptations
- Magnetic and Electromagnetic Effects
Chang'an University
2022-2024
University of California, San Francisco
2019-2023
Marquette University
2013-2022
Universidad de Antioquia
2022
Amity University
2022
University of Allahabad
2022
University of San Francisco
2021
Sichuan University
2014
The microtubule (MT) plus-end tracking protein EB1 is present at the tips of cilia and flagella; end-binding 1 (EB1) remains tip during flagellar shortening in absence intraflagellar transport (IFT), predominant system flagella. To investigate how accumulates tip, we used vivo imaging fluorescent protein-tagged (EB1-FP) Chlamydomonas reinhardtii. After photobleaching, signal recovered within minutes, indicating an exchange with unbleached entering flagella from cell body. moved independent...
Intraflagellar transport (IFT) carries proteins into flagella but how IFT trains interact with the large number of diverse required to assemble remains largely unknown. Here, we show that radial spokes in Chlamydomonas requires ARMC2/PF27, a conserved armadillo repeat protein associated male infertility and reduced lung function. ARMC2 was highly enriched growing tagged spoke RSP3 co-migrated on anterograde trains. In contrast, cargo an adapter inner outer dynein arms moved independently...
Abstract Intracellular pH dynamics is increasingly recognized to regulate myriad cell behaviors. We report a finding that intracellular also regulates adult stem lineage specification. identify an gradient in mouse small intestinal crypts, lowest crypt cells and increasing along the column. Disrupting this by inhibiting H + efflux Na /H exchanger 1 abolishes budding blocks differentiation of Paneth cells, which are rescued with exogenous WNT. Using single-cell RNA sequencing tracing we...
Although microtubules are known for dynamic instability, the dynamicity is considered to be tightly controlled support a variety of cellular processes. Yet diverse evidence suggests that this not applicable Chlamydomonas, biflagellate fresh water green alga, but intense autofluorescence from photosynthesis pigments has hindered investigation. By expressing bright fluorescent reporter protein at endogenous level, we demonstrate in real time discreet sweeping changes algal elicited by rises...
Nucleoside diphosphate kinases (NDKs) play a central role in diverse cellular processes using the canonical NDK activity or alternative mechanisms that remain poorly defined. Our study of dimeric NDK5 flagellar motility control complex, radial spoke (RS), has revealed new modalities. The flagella Chlamydomonas ndk5 mutant were paralyzed, albeit only deficient three RS subunits. morphology appeared severely changed averaged cryo-electron tomograms, suggesting is crucial for intact spokehead...
Microalgae represent an exceptionally diverse but highly specialized group of micro-organisms that adapt to various ecological habitats. Due their unique environment (extremely low temperature and anoxia), the Tibetan Plateau is among regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring lawn. In this study, 52 microalgae from different environments in Qinghai-Tibet were isolated divided into 11 groups based on results restriction fragment length...
<em>Rhodobacter sphaeroides</em> is abundant in natural carotenoids. However, fermentation under light growth conditions for carotenoids biosynthesis time consuming. In the present study, 36 h micro-aerobic was employed to rapidly produce from <em>Rb. Sphaeroides</em>. Orthogonal experiments suggested that optimal process was: temperature 33°C, inoculation amount 8%, duration h. The yield of total 11.542 mg/L. Real-time PCR indicated expression levels <em>crtA</em>, <em>crtD</em>,...
Abstract The microtubule cytoskeletal system is integral to diverse cellular processes. Although microtubules are known for dynamic instability, the tightly controlled in typical interphase animal cells. In contrast, evidence suggests that mercurial unicellular fresh water green alga, Chlamydomonas , but intense autofluorescence from photosynthesis pigments has hindered investigation. By expressing a bright fluorescent reporter protein at endogenous level, we demonstrate real time discreet...
Summary Intracellular pH (pHi) dynamics is increasingly recognized to regulate myriad cell behaviors, including proliferation, migration, differentiation, and transformation. Here we report a new finding that pHi also regulates adult stem lineage specification. In mouse small intestinal organoids, identify gradient along the crypt axis, lower at base higher toward villus, find dissipating this by inhibiting Na + -H exchanger 1 (NHE1) activity genetically or pharmacologically abolishes...
Abstract Intraflagellar transport (IFT) carries proteins into flagella but how IFT trains interact with the large number of diverse required to assemble remains largely unknown. Here, we show that radial spokes in Chlamydomonas requires ARMC2/PF27, a conserved armadillo repeat protein associated male infertility and reduced lung function. ARMC2 was highly enriched growing tagged spoke RSP3 comigrated on anterograde trains. In contrast, cargo an adapter inner outer dynein arms moved...