A5077634952- Tuberculosis Research and Epidemiology
- Biochemical and Molecular Research
- MicroRNA in disease regulation
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Bioinformatics and Genomic Networks
- Cancer therapeutics and mechanisms
- Cancer-related molecular mechanisms research
- Pharmacological Effects of Natural Compounds
- vaccines and immunoinformatics approaches
- Glioma Diagnosis and Treatment
- Microbial Metabolic Engineering and Bioproduction
- Cancer Mechanisms and Therapy
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Cancer Research and Treatment
- ATP Synthase and ATPases Research
- Galectins and Cancer Biology
- Cancer-related Molecular Pathways
- Synthesis and Catalytic Reactions
Capital University of Science and Technology
2017-2025
Lund University
2023
University of North Carolina at Chapel Hill
2016
Mohammad Ali Jinnah University
2009-2012
International Islamic University, Islamabad
2012
University of South Australia
2005
Background: The global burden of tuberculosis (TB) and antibiotic resistance is attracting the attention researchers to develop some novel rapid diagnostic tools. Although, conventional methods like culture are considered as gold standard, they time consuming in procedure, during which there more chances transmission disease. Further, Xpert MTB/RIF assay offers a fast facility within 2 h, but due low sensitivity sample types may lead serious state role computer technologies now increasing...
Resistance to key first-line drugs is a major hurdle achieve the global end tuberculosis (TB) targets. A prodrug, pyrazinamide (PZA) only drug, effective in latent TB, recommended drug resistance and susceptible Mycobacterium (MTB) isolates. The prodrug conversion into active form, pyrazinoic acid (POA), required activity of pncA gene encoded pyrazinamidase (PZase). Although mutations have been commonly associated with PZA but small number cases mutationss RpsA protein. Here this study total...
Pyrazinamide (PZA) is an important component of first-line drugs because its distinctive capability to kill subpopulations persistent Mycobacterium tuberculosis (MTB). The prodrug converted active form, pyrazinoic acid (POA) by MTB pncA-encoded pyrazinamidase (PZase). Mutation in pncA the most common and primary cause PZA resistance. aim present study was explore molecular characterization resistance a Pashtun-dominated region Khyber Pakhtunkhwa, Pakistan.We performed drug susceptibility...
Severe acute respiratory syndrome virus 2 (SARS-CoV-2) belongs to the single-stranded positive-sense RNA family. The contains a large genome that encodes four structural proteins, small envelope (E), matrix (M), nucleocapsid phosphoprotein (N), spike (S), and 16 nonstructural proteins (nsp1-16) together, ensure replication of in host cell. Among these interactions N Nsp3 are essential links viral for processing. reside at CoV synthesis sites known as replication-transcription complexes...
ABSTRACT Bedaquiline is a key drug recommended by the WHO for treatment of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant (XDR) TB, its use could potentially shorten duration with improved outcomes. However, resistance against this has increased, resulting in serious concerns. Heteroresistance among important obstacles to complicate detection MTB resistance. We investigated presence bedaquiline heteroresistant isolates from Pakistan highlight their relevance Whole...
Abstract Glioblastoma multiforme (GBM) IDH-wildtype is the most prevalent brain malignancy in adults. However, molecular mechanisms, which leads to GBM have not been completely elucidated. Granulocyte colony-stimulating factor (GCSF), receptor GCSFR, and Signal transducers activators of transcription 3 (STAT3) involved occurrence development various cancers, but their role little known. Herein, we investigated gene protein expression GCSF, STAT3 21 tissue biopsy samples also tumor associated...
Pyrazinamide (PZA) is an important component of first-line anti-tuberculosis drugs which converted into active form, pyrazinoic acid (POA), by Mycobacterium tuberculosis (MTB) pncA gene encoded, pyrazinamidase (PZase). Mutations in are detected >70% PZA resistant isolates but, noticeably, not all. In this study, we selected 18 PZA-resistant but wild type (pncAWT) MTB isolates. Drug susceptibility testing (DST) all the were repeated at critical concentration drug. All these PZA-resistance...
Pyrazinamide (PZA) is an essential first line antitubercular drug, which plays a crucial role in tuberculosis treatment. The PZA, considered as pro-drug needs enzyme of mycobacterial pyrazinamidase (PZase) for its conversion into active form pyrazinoic acid. Further, this PZA inhibits the ribosomal proteins S1, facilitates transfer-mRNA complex formation throughout translation. spontaneous mutations RpsA have been found to be associated with drug resistance. However, resistance mechanism...
In an effort to discover the mechanism of resistance offered by Mycobacterium tuberculosis (Mtb) toward pyrazinamide (PZA) drug, extensive molecular dynamics strategy was employed. PZA is a first-line prodrug that effectively cuts therapy time 33% (from 9 6 months). Pyrazinamidase enzyme (PZase), encoded pncA gene, responsible for activation into pyrazinoic acid (POA). POA toxic and potently inhibits growth latent Mtb even at low pH values. caused three genes pncA, rpsA, panD. Among them,...
A central approach for better understanding the forces involved in maintaining protein structures is to investigate folding and thermodynamic properties. The effect of process often disturbed mutated states. To explore dynamic properties behind mutations, molecular (MD) simulations have been widely performed, especially unveiling mechanism drug failure mutation. When comparing wild type (WT) mutants (MTs), structural changes along with solvation free energy (SFE), Gibbs (GFE) are calculated...
Pyrazinamide (PZA) is an important component of first-line tuberculosis (TB) treatment because its distinctive capability to kill subpopulations persister Mycobacterium (MTB). The significance PZA can be understood by inclusion in the most recent World Health Organization-recommended multidrug-resistant (MDR) TB regimen. Very little information available about prevalence PZA-resistant from geographically distinct regions high burden countries, including Khyber Pakhtunkhwa (KPK), Pakistan,...
Background: Clostridium tetani are rod-like, anaerobic types of pathogenic bacteria the genus Clostridium. It is Gram-positive in nature and appears as a tennis racket or drumsticks on staining with dye. Tetanus neuromuscular disease wherein exotoxin produces muscle fits host. second leading cause worldwide deaths occurring from family immunization-preventable diseases. Methods: In this research, subtractive proteome analysis C. was performed to identify putative drug targets. The proteins...
Pyrazinamide (PZA) is an important component of first-line antituberculosis drugs activated by Mycobacterium tuberculosis pyrazinamidase (PZase) into its active form pyrazinoic acid. Mutations in the pncA gene have been recognized as major cause PZA resistance. We detected some novel mutations, Leucine19Arginine (L19R), Arginine140Histidine (R140H), and Glutamic acid144 Lysine (E144K), PZA-resistant isolates our wet lab drug susceptibility testing sequencing. As molecular mechanism...
Pyrazinamide (PZA) is an important component of first-line anti-tuberculosis (anti-TB) drugs. The anti-TB agent activated into active form, pyrazinoic acid (POA), by Mycobacterium tuberculosis (MTB) pncA gene encoding pyrazinamidase (PZase). major cause PZA-resistance has been associated with mutations in the gene. We have detected several novel including V131F, Q141P, R154T, A170P, and V180F (GeneBank Accession No. MH461111) PZA-resistant isolates during PZA drug susceptibility testing...
Osteoarthritis occurs when protective cartilage of bones worn out. Similarlty, damage mainly in the pannus rheumatoid arthritis. It is a potentially debilitating condition, affecting women two to three times more often than men. The cause and prognosis osteoarthritis are still poorly known. However, advances study disease pathogenesis have encouraged creation new therapeutics with improved outcomes. purpose this investigate differentially expressed genes involved dysregulated arthritis (RA)...
Glioblastoma (GBM) harbors significant genetic heterogeneity, high infiltrative capacity, and patterns of relapse following many therapies. The expression nuclear factor kappa-B (NF-κB p65 (RelA)) signaling pathways is constitutively activated in GBM through inflammatory stimulation such as tumor necrosis factor-alpha (TNFα), cell invasion, motility, abnormal physiological stimuli, inducible chemoresistance. However, the underlying anti-tumor anti-proliferative mechanisms NF-κB (RelA) TNFα...