A5022230566- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Cocoa and Sweet Potato Agronomy
- Research in Cotton Cultivation
- Plant-Microbe Interactions and Immunity
- Agricultural pest management studies
- Fungal Plant Pathogen Control
- Plant Parasitism and Resistance
- Plant Pathogens and Resistance
- Plant pathogens and resistance mechanisms
- Genetic and Environmental Crop Studies
- Agricultural Practices and Plant Genetics
- Plant Disease Resistance and Genetics
- Agriculture, Plant Science, Crop Management
- Plant Disease Management Techniques
- Soybean genetics and cultivation
- Insect Resistance and Genetics
- Botanical Research and Chemistry
- Powdery Mildew Fungal Diseases
- Peanut Plant Research Studies
- Biological Control of Invasive Species
Nuclear Institute for Agriculture and Biology
2015-2025
Pakistan Institute of Engineering and Applied Sciences
2024-2025
National University of Sciences and Technology
2020-2021
Leaf curl disease caused by Cotton Curl Burewala virus (CLCuBuV) has been recognized as serious threat to cotton in Indian subcontinent. However, information about cotton–CLCuBuV interaction is still limited. In this study, the level of phenolic compounds, total soluble proteins, and malondialdehyde (MDA) activities phenylalanine ammonia-lyase (PAL), peroxidase (POX), catalase (CAT), proteases, superoxide dismutase (SOD), polyphenol oxidase (PPO) were studied leaves two susceptible (CIM-496...
Abstract Cotton leaf curl disease (CLCuD), caused by cotton viruses (CLCuVs), is among the most devastating diseases in cotton. While widely cultivated species Gossypium hirsutum generally susceptible, diploid G. arboreum a natural source for resistance against CLCuD. However, influence of CLCuD on transcriptome and interaction with remains to be elucidated. Here we have used an RNA-Seq based study analyze differential gene expression under infestation. plants were infested graft inoculation...
Resistance to key first-line drugs is a major hurdle achieve the global end tuberculosis (TB) targets. A prodrug, pyrazinamide (PZA) only drug, effective in latent TB, recommended drug resistance and susceptible Mycobacterium (MTB) isolates. The prodrug conversion into active form, pyrazinoic acid (POA), required activity of pncA gene encoded pyrazinamidase (PZase). Although mutations have been commonly associated with PZA but small number cases mutationss RpsA protein. Here this study total...
ABSTRACT Mungbean is an economically important short‐duration traditional pulse crop in Pakistan. During a routine inspection of mungbean fields the summer 2018, unusual disease symptoms reminiscent Tospovirus were observed for first time. These include severe leaf chlorosis, necrosis, plant stunting and death. To identify causal virus, symptomatic fresh leaves analysed following RT‐PCR using GBNV‐specific primer pair which resulted amplification ~0.8 kbp fragment specific to CP gene...
Cotton leaf curl disease (CLCuD), caused by cotton Burewala virus (CLCuBV), has emerged as a major threat to production in Pakistan. Resistance CLCuBV was evaluated cultivated and wild genotypes representing six Gossypium species visual symptom scoring assessment using PCR tests. Considerable variation responses observed when whitefly graft transmission inoculate with field greenhouse experiments. Under evaluation, all of hirsutum three G. barbadense were susceptible. Eleven that represented...
Yellow mosaic disease (YMD) is one of the major diseases affecting mungbean (Vigna radiata (L.) Wilczek). In this study, we report mapping quantitative trait locus (QTL) for yellow India virus (MYMIV) resistance in mungbean. An F8 recombinant inbred line (RIL) population was generated Thailand from a cross between NM10-12-1 (MYMIV resistance) and KPS2 susceptible). One hundred twenty-two RILs their parents were evaluated MYMIV infested fields Pakistan. A genetic linkage map developed RIL...
Severe acute respiratory syndrome virus 2 (SARS-CoV-2) belongs to the single-stranded positive-sense RNA family. The contains a large genome that encodes four structural proteins, small envelope (E), matrix (M), nucleocapsid phosphoprotein (N), spike (S), and 16 nonstructural proteins (nsp1-16) together, ensure replication of in host cell. Among these interactions N Nsp3 are essential links viral for processing. reside at CoV synthesis sites known as replication-transcription complexes...
Phyllody is a serious disease of sesame in Pakistan. In the present study investigations were carried out on symptomatology, etiology, and transmission this disease. Floral virescence, phyllody, proliferation are most common symptoms. Sometimes these symptoms accompanied by yellowing, cracking seed capsules, germination seeds formation dark exudates foliage. Shoot apex fasciation has also been occasionally observed, but no phytoplasma DNA detected fasciated plants using PCR assays. Light...
During spring 2005–06 chickpea plants (cvs Desi and Kabuli) were found to be affected by a previously undescribed disease in Pakistan. Symptoms consisted of proliferation branches with smaller leaflets, giving bushy appearance the plants. Affected scattered field more easily spotted at flowering podding time. The flowers developed abnormal green structures (phyllody) instead normal flowers. At time crop maturity when healthy drying diseased conspicuously green. Tissue samples from without...
Studies were conducted to identify the sources of resistance in mungbean recombinant inbred lines (RILs) Thailand against yellow mosaic disease (MYMD). 146 RILs <TEX>$F_8$</TEX> series evaluated a field including resistant parent NM-10-12-1 and susceptible KPS 2 during summer 2008 under high inoculum pressure. The subsequently scored for symptom severity ratings (DSSR) using new scale. Observations regarding DSSR % index (%DI) showed that tested responded differently disease. A large number...
Abstract Objective Phyllody disease caused by phytoplasma is an emerging problem in mungbean worldwide. However, the alterations host physiology and its associated biochemical components induced infection with plant remain unknown. Hence present study was performed diseased plants order to determine patho-physiological changes that take place. Methods Under study, total phenolic compounds, soluble proteins, peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO), Chlorophyll a, b,...
Sesame phyllody disease has been recorded on sesame in Pakistan for a number of years, and is characterized by virescence, phyllody, yellowing, floral sterility stem proliferation infected plants. The causes significant losses (Sarwar et al., 2006) but prior to this report the causal agent had not identified. However, Oman, phytoplasmas 16SrII group have reported as (Al-Sakeiti 2005). Tissue samples from uninfected plants were examined using light microscope Dienes' stain. Regularly...
Charcoal rot caused by Macrophomina phaseolina is a serious disease of sesame in Pakistan. M. isolate was subjected to growth rate test at 10, 15, 20, 25, 30, 35 and 40°C. The optimum temperature for fungal microsclerotia production found be 30–35°C. Gray black, radial colonies with intermediate mycelial jet black oval round were observed this range. pathogenic against all the 18 tested plant species pathogenicity proved its necrophytic behavior. Seed infection efficiency 100% significant...
Chickpea chlorotic dwarf virus (CpCDV, genus Mastervirus, family Geminiviridae) is the most common viral disease of chickpea in Pakistan. Two aphid [Aphis craccivora Koch, Myzus persicae (Sulzer)], two leafhopper [Empoasca devastans Distant, Orosius albicinctus (Distant)] species and an unidentified brown were collected a field by hand sweep nets for transmission studies CpCDV. Transmission results showed that only O. successfully transmitted CpCDV from diseased to healthy plants. The...