A5072067307- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Plant Pathogens and Fungal Diseases
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Entomopathogenic Microorganisms in Pest Control
- Banana Cultivation and Research
- Mycotoxins in Agriculture and Food
- Plant and Fungal Interactions Research
- Plant Parasitism and Resistance
- Insect and Pesticide Research
- Plant Disease Management Techniques
- Biosensors and Analytical Detection
- Insect and Arachnid Ecology and Behavior
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Collembola Taxonomy and Ecology Studies
- Hemiptera Insect Studies
- Nematode management and characterization studies
- Research on scale insects
- Diphtheria, Corynebacterium, and Tetanus
- Insect Pest Control Strategies
- Legume Nitrogen Fixing Symbiosis
Elsoms (United Kingdom)
2019-2022
Fera Science (United Kingdom)
2010-2020
University of Nottingham
2006-2014
Phytoplasma phylogenetics has focused primarily on sequences of the non-coding 16S rRNA gene and 16S–23S intergenic spacer region (16–23S ISR), primers that enable amplification these regions from all phytoplasmas by PCR are well established. In this study, based secA have been developed into a semi-nested assay results in sequence expected size (about 480 bp) 34 examined, including strains representative 12 16Sr groups. Phylogenetic analysis showed similar clustering when compared with...
In Europe the most devastating phytoplasma associated with grapevine yellows ( GY ) diseases is a quarantine pest, flavescence dorée FD p), from 16SrV taxonomic group. The on‐site detection of p an affordable device would contribute to faster and more efficient decisions on control measures for p. Therefore, real‐time isothermal LAMP assay was validated according EPPO standards MIQE guidelines. shown be specific extremely sensitive, because it detected in all leaf samples that were...
The yellow-legged or Asian hornet (Vespa velutina colour form nigrithorax) was introduced into France from China over a decade ago. Vespa has since spread rapidly across Europe, facilitated by suitable climatic conditions and the ability of single nest to disperse many mated queens large area. Yellow-legged hornets are major concern because potential impact they have on populations beneficial pollinators, most notably western honey bee (Apis mellifera), which shows no effective defensive...
Primers and probes based on the 23S rRNA gene have been utilized to design a range of real-time PCR assays for routine phytoplasma diagnostics. These authenticated as specific shown be at least sensitive nested PCR. A universal assay detect all phytoplasmas has developed, along with multiplex discriminate 16SrI group from members other 16Sr groups. Assays 16SrII, 16SrIV, 16SrXII groups also developed confirm that can used group-specific assays.
A study to investigate the association of phytoplasmas with papaya dieback and citrus decline syndromes in Ethiopia was carried out between July 2009 February 2010, sampling performed major papaya‐ citrus‐growing areas Rift Valley. Samples plants symptoms were collected from papaya, suspected phytoplasma weed hosts crops around fields studied. Nested polymerase chain reaction (nested‐PCR) used for initial characterization, using primers that amplify regions 16S rRNA secA genes, results then...
Since its conception, DNA barcoding has seen a rapid uptake within the research community. Nevertheless, as with many new scientific tools, progression towards point of routine deployment diagnostic laboratories been slow. In this paper, we discuss application in Defra plant health laboratories, where is used primarily for identification invertebrate pests. We present series case studies that demonstrate successful but also reveal some potential limitations to expanded use. The regulated...
Bakanae disease (caused by Fusarium fujikuroi) and rice blast Magnaporthe oryzae) are two of the most important seedborne pathogens rice. The detection both in seed is necessary to maintain high quality standards avoid production losses. Currently, blotter tests used followed morphological identification developing provide an incidence infection lots. Two loop-mediated isothermal amplification assays were developed with primers designed target elongation factor 1-α sequence F. fujikuroi...
The larvae of the Old World genera Leucinodes Guenée, 1854 and Sceliodes are internal feeders in fruits Solanaceae, causing economic damage to cultivated plants like Solanum melongena S. aethiopicum. In sub-Saharan Africa five nominal species one occur. One these species, eggplant fruit shoot borer L. orbonalis 1854, is regarded as regularly intercepted from Asia Europe, North South America therefore a quarantine pest on continents. We investigate taxonomy African based morphological...
A terminal restriction fragment analysis (T‐RFLP) technique was developed for the simple and rapid detection diagnosis of phytoplasmas in plants. The selected primers amplified part 23S rRNA gene to provide improved resolution between taxonomic groups compared conventional enzyme 16S rRNA. Using enzymes Bsh 12361 Mse I on PCR products, range 68–640 bp, tested 37 isolates from 10 16Sr groups. Distinct unambiguous T‐RFLP profiles were produced nine groups, such that almost all within a group...
Xanthomonas campestris pv. musacearum (Xcm) is the causal agent of banana xanthomonas wilt, a major threat to production in eastern and central Africa. The pathogen present very high levels within infected plants can be transmitted by broad range mechanisms; therefore early specific detection vital for effective disease management. In this study, polyclonal antibody (pAb) was developed deployed lateral flow device (LFD) format allow rapid in-field Xcm. Published Xcm PCR assays were also...
Journal Article Rapid, specific, simple, in‐field detection of Xanthomonas campestris pathovar musacearum by loop‐mediated isothermal amplification Get access J. Hodgetts, Hodgetts Fera, National Agri‐Food Innovation Campus York UK Correspondence Jennifer Campus, Sand Hutton, YO41 1LZ York, UK. E‐mail: jennifer.hodgetts@fera.co.uk Search for other works this author on: Oxford Academic Google Scholar Hall, Hall G. Karamura, Karamura Agricultural Research Laboratories PO Box 7064 Kampala...
Abstract Two independent surveys were performed in Peru during February and November 2007 to detect the presence of phytoplasmas within any crops showing symptoms resembling those caused by phytoplasmas. Molecular identifications characterisations based on phytoplasma 16S 23S rRNA genes using nested PCR terminal restriction fragment length polymorphism (T‐RFLP). The indicated that present most locations sampled both cultivated crops, including carrots, maize, native potatoes, improved...
Mung bean is an important source of food for humans and animals in tropical subtropical countries, also used green manure. During spring 2008, mung plants Faisalabad, Pakistan, showed severe symptoms phyllody, virescence a bushy appearance. Stem, leaf stalk phloem sections from with without were examined under light microscope (CXRII, Labomed, CA, USA) using Dienes’ stain. Regularly distributed dark blue areas observed only the cells symptoms, not other plant tissues nor healthy plants,...
Bacterial blotch is a group of economically important diseases the common button mushroom (Agaricus bisporus). Once pathogens are introduced to farm, mesophilic growing conditions (that optimum for production) result in severe and widespread secondary infections. Efficient, timely quantitative detection hence critical design localized control strategies prediction disease risk. This study describes development real-time TaqManTM assays that allow molecular diagnosis three currently prevalent...
Rose cryptic virus-1 (RoCV1) also known as Rosa multiflora virus is a partitivirus affecting roses, one of the most important ornamental crops worldwide. RoCV1 has previously been reported in US, Canada and New Zealand, now identified United Kingdom for first time. Using High Throughput Sequencing (HTS) sequences were found two samples collected 2007 2012. This discovery led to development RT-qPCR (TaqMan) assay detection this virus. As part rose survey UK, 251 analysed using newly developed...