A5078577158- Stress Responses and Cortisol
- Adrenal Hormones and Disorders
- Neuroendocrine regulation and behavior
- Birth, Development, and Health
- Growth Hormone and Insulin-like Growth Factors
- Hemoglobinopathies and Related Disorders
- Hypothalamic control of reproductive hormones
- Electrolyte and hormonal disorders
- Pregnancy and preeclampsia studies
- Ovarian function and disorders
- Prenatal Screening and Diagnostics
- Neonatal Respiratory Health Research
- Iron Metabolism and Disorders
- Preterm Birth and Chorioamnionitis
- Hormonal Regulation and Hypertension
- Reproductive Biology and Fertility
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- Reproductive System and Pregnancy
- Vitamin D Research Studies
- Regulation of Appetite and Obesity
- Thyroid Disorders and Treatments
- Pituitary Gland Disorders and Treatments
- Neuroblastoma Research and Treatments
Louisiana State University
2022-2025
Johns Hopkins University
2020-2024
Massachusetts Institute of Technology
2024
Icahn School of Medicine at Mount Sinai
2017-2020
John Radcliffe Hospital
1995-2017
University of Oxford
2000-2017
New York University
2016
Monklands Hospital
2010
NHS Lanarkshire
2010
University of Siena
2002-2006
Objective To investigate whether syncytiotrophoblast microvilli (STBM) are shed into the maternal circulation in increased amounts pre‐eclamptic pregnancies as a possible cause of vascular endothelial dysfunction. Design A time‐resolved fluoroimmunoassay was developed to measure STBM levels peripheral and uterine venous plasma from normal pregnant women. Three colour flow cytometry used assess microparticulate nature pregnancy plasma. The effects these plasmas on cell proliferation compared...
Plasma CRH was measured in maternal plasma throughout the third trimester of pregnancy, during labor, and postpartum. levels were also arterial venous umbilical. cord samples. In normal pregnant women, increased from 50 ± 15 (±sem) pg/mL at 28 weeks gestation (n = 41) to 1462 182 40 55) 1680 101 65) labor. Women with pregnancy-induced hypertension 49) had significantly elevated above this range. Similarly, women who subsequently went into premature labor raised several before onset After...
In recent studies to clone and characterize genes coding for the corticotropin-releasing factor-binding protein (CRF-BP), analysis of tissue distribution CRF-BP gene indicated a high level expression in rat brain. We have now characterized by immunohistochemical hybridization histochemical means cellular localization mRNA expression, respectively. Results from both approaches converged indicate that is expressed predominantly cerebral cortex, including all major archi-, paleo-, neocortical...
Objective To determine whether circulating markers of oxidative stress are elevated in pre‐eclampsia when appropriate precautions taken to prevent vitro oxidation Design A prospective study. Setting Nuffield Department Obstetrics and Gynaecology, Oxford The William Harvey Institute, London. Sample Three groups women: those with ( n = 19 ), control pregnant ) matched for gestation, age parity a group non individuals reproductive (n 7). Methods Citrated plasma was stored at −80°C 20 μmol β...
In pregnancy, maternal plasma corticotropin releasing hormone (CRH) concentrations rise substantially in the third trimester and fall rapidly post-partum. A binding protein (BP) specific for CRH exists human circulation which inactivates CRH, thus possibly explaining why ACTH does not outside normal limits throughout gestation. We here describe measurement of CRH-BP directly during pregnancy using a radioimmunoassay that is affected by presence high levels occur at this time. 119 healthy...
Summary. Maternal plasma levels of cortiocotrophin‐releasing factor (CRF) have been measured in abnormal pregnancy states to assess their potential as biochemical markers for at‐risk pregnancies. CRF were not significantly altered patients with hydatidiform mole, polyhydramnios or diabetes. elevated pregnancies complicated by accidental antepartum haemorrhage at 28 weeks (P<0·03) but the rest third trimester. In twin raised throughout trimester (28–32 weeks, P<0·01; 34–36 P<0·001)....
Abstract Background Older adult delirium is often unrecognized in the emergency department (ED), yet most compelling research questions to overcome knowledge‐to‐practice deficits remain undefined. The Geriatric Emergency care Applied Research (GEAR) Network was organized identify and prioritize clinical questions. Methods GEAR identified engaged 49 transdisciplinary stakeholders including physicians, geriatricians, nurses, social workers, pharmacists, patient advocates. Adhering Preferred...
ABSTRACT We report the purification of human corticotrophin-releasing factor-binding protein (hCRF-BP) using repeated affinity chromatography (with aid synthetic CRF immobilized on Sepharose 4B solid phase) followed by gel filtration. Presence binding was tracked throughout procedure its ability to inhibit 125 I-labelled hCRF an antiserum; normal plasma exhibits 80% inhibition in this system whereas sheep plasma, which does not contain hCRF-BP, has no effect. Affinity cross-linking purified...
Intact handled rats were pretreated with the immunoglobulin G fractions from normal rabbit serum or antisera to ovine corticotropin-releasing factor (CRF) and/or vasopressin and subjected restraint formalin stress. The formalin- induced rise in plasma ACTH was reduced 28% anti-CRF, 53% those antivasopressin, 16% given both antibodies. Pretreatment of animals a combination antibodies also attenuated response stress 13%, 37%, 12%, respectively, serum-treated rats. Antiserum pretreatment did...
Late gestational maternal plasma contains a carrier substance for corticotrophin releasing factor (CRF-41) with molecular weight in the region of 40,000. Using gel chromatography and CRF-41 immunoradiometric assay (IRMA), we show that binding peptide to its can be disrupted by treatment urea. The capacity is not saturated, since time-dependent incorporation synthetic occurs at 4 degrees C. also present normal male plasma. It specific human CRF-41, ACTH, GnRH, vasopressin or ovine CRF-41....
This study is the first to use CRH-binding protein (CRH-BP) purified from human plasma investigate how CRH-BP affects physiological activity of CRH. After incubation at 37 C for 15 min, reduced ACTH-releasing synthetic (h) CRH in a dose-dependent fashion; using hCRH and concentrations commonly found late gestational maternal plasma, (1.5 100 ng/mL, respectively) an average 76% reduction ACTH release was obtained. No effect observed under same conditions on induced by ovine (o) CRH, which...
Abstract The purpose of this study was to examine the effects differential reinforcement other behavior (DRO) on reducing unsafe playground young children at school and subsequently, if necessary, additive a brief time‐out. DRO procedure effective in eliminating for one four participants. three participants experienced addition time‐out combination with DRO. + TO condition nearly eliminated all who condition. Additionally, did not negatively affect social interactions among peers or...
A “two-site” immunoradiometric assay (IRMA) which allows the direct estimation of human CRH (hCRH) in plasma is described. Using this IRMA, basal levels normal subjects ranged from 2–28 pg/mL [mean, 15 ± 7 (±sd) pg/mL; n = 58]. Values men and women were similar. Plasma values within range also found patients with Cushing’s syndrome, Addison’s disease, Nelson’s no correlation between ACTH these patients. Elevated pregnant near term [1462 752 55], dilution curve CRH-like immunoreactivity...
Abstract Background Sickle cell disease (SCD) is an autosomal recessive blood disorder affecting approximately 100,000 Americans and 3.1 million people globally. The scarcity of relevant knowledge experience with rare diseases creates a unique need for cooperation infrastructure to overcome challenges in translating basic research advances into clinical advances. Despite registry initiatives SCD, the unavailability descriptions selection process copies final data collection tools, coupled...
The frequency, severity, and forms of symptoms months after coronavirus 2019 (COVID-19) are poorly understood, especially in community settings. To better understand characterize community-based COVID-19, a retrospective cohort analysis was conducted. Three hundred twenty-eight consecutive persons with positive test for SARS-CoV-2 the Johns Hopkins Health System, Maryland, March-May 2020, were selected study. Symptom occurrence severity measured through questionnaires. Of 328 evaluated,...
Samples of maternal blood, amniotic fluid and umbilical arterial venous blood were collected from 11 women at 16-24 weeks pregnancy. Corticotrophin-releasing hormone-41 (CRH-41) ACTH measured by immunoradiometric assay. The mean levels pmol/l in plasma, 12 fetal plasma 9.7 fluid. CRH-41 1.6 0.7 plasma. There was a positive correlation between ACTH. In there weak inverse This is the first demonstration circulation mid-trimester human fetus, but on basis present findings it not possible to...
In Brief OBJECTIVE: Urocortin is a neuropeptide, member of the corticotropin-releasing hormone family, that produced by human endometrium. Ovarian endometrioma prevalent gynecologic disorder still lacking specific serum markers. present study we measured systemic levels urocortin to assess diagnostic performance its determination in distinguishing endometriomas from other benign ovarian cysts. METHODS: Plasma was radioimmunoassay women with (n=40) and benign, nonendometriotic cysts (n=40)....
Sexual and reproductive healthcare standards for adolescents young adults with sickle cell disease (SCD) are not established. A total of 50 entering adult SCD care completed a Family Planning Survey assessing sexual health needs from March 2019 to July 2020. Clinical data were abstracted respondents' electronic medical records. Linear logistic regression was applied explore associations between clinical characteristics survey results. Few respondents (8%) wished be pregnant in the coming...