A5064827512- Cellular Mechanics and Interactions
- Spaceflight effects on biology
- Microfluidic and Bio-sensing Technologies
- Mathematics and Applications
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Gastrointestinal Tumor Research and Treatment
- 3D Printing in Biomedical Research
- Liver physiology and pathology
- Cardiomyopathy and Myosin Studies
- Axon Guidance and Neuronal Signaling
- Hedgehog Signaling Pathway Studies
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Micro and Nano Robotics
- Point processes and geometric inequalities
Princeton University
2018-2024
University of California, Santa Barbara
2022-2024
During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes local cellular processes, global coordination of remodeling at the organ scale remains unclear. Here, we combine in toto light-sheet microscopy Drosophila embryo with quantitative analysis physical modeling to relate patterns force generation during gastrulation process. We find that complex spatio-temporal pattern can be predicted...
The actomyosin cytoskeleton is a crucial driver of morphogenesis. Yet how the behavior large-scale cytoskeletal patterns in deforming tissues emerges from interplay geometry, genetics, and mechanics remains incompletely understood. Convergent extension Drosophila melanogaster embryos provides opportunity to establish quantitative understanding dynamics anisotropic non-muscle myosin II. Cell-scale analysis protein localization fixed suggests that gene expression govern anisotropy via complex...
Shape changes of epithelia during animal development, such as convergent extension, are achieved through the concerted mechanical activity individual cells. While much is known about corresponding large-scale tissue flow and its genetic drivers, fundamental questions regarding local control contractile on cellular scale embryo-scale coordination remain open. To address these questions, we develop a quantitative, model-based analysis framework to relate cell geometry tension in recently...
During morphogenesis, diverse cell-scale and tissue-scale processes couple to dynamically sculpt organs. In this coupling, genetic expression patterns biochemical signals regulate respond mechanical deformations ensure reproducible robust changes in tissue geometry. A long-standing approach characterize these interactions has been the construction of atlases, atlases have necessarily relied on fixed snapshots embryogenesis. Addressing how profiles relate dynamics, however, requires a scheme...
Shape changes of epithelia during animal development, such as convergent extension, are achieved through concerted mechanical activity individual cells. While much is known about the corresponding large scale tissue flow and its genetic drivers, fundamental questions regarding local control contractile on cellular embryo-scale coordination remain open. To address these questions, we develop a quantitative, model-based analysis framework to relate cell geometry tension in recently obtained...
Abstract Shape changes of epithelia during animal development, such as convergent extension, are achieved through concerted mechanical activity individual cells. While much is known about the corresponding large scale tissue flow and its genetic drivers, fundamental questions regarding local control contractile on cellular embryo-scale coordination remain open. To address these questions, we develop a quantitative, model-based analysis framework to relate cell geometry tension in recently...
The actomyosin cytoskeleton is a crucial driver of morphogenesis. Yet how the behavior large-scale cytoskeletal patterns in deforming tissues emerges from interplay geometry, genetics, and mechanics remains incompletely understood. Convergent extension flow D. melanogaster embryos provides opportunity to establish quantitative understanding dynamics anisotropic non-muscle myosin II. Cell-scale analysis protein localization fixed suggests that there are complex rules governing control...
During embryogenesis tissue layers continuously rearrange and fold into specific shapes. Developmental biology identified patterns of gene expression cytoskeletal regulation underlying local dynamics, but how actions multiple domains distinct cell types coordinate to remodel tissues at the organ scale remains unclear. We use in toto light-sheet microscopy, automated image analysis, physical modeling quantitatively investigate link between kinetics global transformations force generation...
Shape changes of epithelia during animal development, such as convergent extension, are achieved through the concerted mechanical activity individual cells. While much is known about corresponding large-scale tissue flow and its genetic drivers, fundamental questions regarding local control contractile on cellular scale embryo-scale coordination remain open. To address these questions, we develop a quantitative, model-based analysis framework to relate cell geometry tension in recently...