A5003032786- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Endoplasmic Reticulum Stress and Disease
- Renal and related cancers
- Psoriasis: Treatment and Pathogenesis
- Biomedical Ethics and Regulation
- Epigenetics and DNA Methylation
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Single-cell and spatial transcriptomics
- Genomic variations and chromosomal abnormalities
- RNA Interference and Gene Delivery
- Hematological disorders and diagnostics
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Mitochondrial Function and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Blood disorders and treatments
- Acute Myeloid Leukemia Research
- Genomics and Chromatin Dynamics
- Neutropenia and Cancer Infections
Salk Institute for Biological Studies
2009-2025
Cedars-Sinai Medical Center
2023-2025
University of Notre Dame
2014-2023
Cancer Research Institute
2019-2020
The University of Texas MD Anderson Cancer Center
2002-2010
The Graduate Center, CUNY
2008
University of Houston
2006-2007
St. Jude Children's Research Hospital
2006
Gladstone Institutes
2006
Boston Children's Hospital
2006
Interleukin-17 (IL-17)-producing helper T (TH) cells, named as THIL-17, TH17, or inflammatory TH (THi), have been recently identified a novel effector lineage. However, how cytokine signals mediate THi differentiation is unclear. We found that IL-6 functioned to up-regulate IL-23R and IL-23 synergized with in promoting generation. STAT3, activated by both IL-23, plays critical role development. A hyperactive form of STAT3 promoted development, whereas this process was greatly impaired...
Th17 and regulatory T (Treg) cells play opposite roles in autoimmune diseases. However, the mechanisms underlying their proper migration to inflammatory tissues are unclear. In this study, we report that these two cell subsets both express CCR6. CCR6 expression is regulated by TGF-beta requires nuclear receptors, RORalpha RORgamma. also ligand CCL20, which induced synergistically IL-6, STAT3, RORgamma IL-21. cells, producing promote of Treg vitro a CCR6-dependent manner. Lack reduces...
Large-scale collections of induced pluripotent stem cells (iPSCs) could serve as powerful model systems for examining how genetic variation affects biology and disease. Here we describe the iPSCORE resource: a collection systematically derived characterized iPSC lines from 222 ethnically diverse individuals that allows both familial association-based studies. are with high genomic integrity (no or low numbers somatic copy-number variants) determined using high-throughput RNA-sequencing...
Generation of human induced pluripotent stem cells (hiPSCs) by the expression specific transcription factors depends on successful epigenetic reprogramming to a state. Although hiPSCs and embryonic (hESCs) display similar epigenome, recent reports demonstrated persistence marks from somatic cell type origin aberrant methylation patterns in hiPSCs. However, it remains unknown whether use different sources, encompassing variable levels selection pressure during reprogramming, influences level...
Stem cells exist in vitro a spectrum of interconvertible pluripotent states. Analyzing hundreds hiPSCs derived from different individuals, we show the proportions these states vary considerably across lines. We discover 13 gene network modules (GNMs) and regulatory (RNMs), which are highly correlated with each other suggesting that coordinated co-accessibility elements RNMs likely underlie expression genes GNMs. Epigenetic analyses reveal networks underlying self-renewal pluripotency more...
Abstract Infection of mouse macrophages by Toxoplasma gondii renders the cells resistant to proinflammatory effects LPS triggering. In this study, we show that cell invasion is accompanied rapid and sustained activation host STAT3. Activation STAT3 did not occur with soluble T. extracts or heat-killed tachyzoites, demonstrating a requirement for live parasites. Parasite-induced phosphorylation suppression LPS-triggered TNF-α IL-12 was intact in IL-10-deficient macrophages, ruling out role...
Abstract Danon disease is a familial cardiomyopathy associated with impaired autophagy due to mutations in the gene encoding lysosomal-associated membrane protein type 2 (LAMP-2). Emerging evidence has highlighted importance of regulating cardiomyocyte bioenergetics, function, and survival. However, mechanisms responsible for cellular dysfunction death cardiomyocytes autophagic flux remain unclear. To investigate molecular disease, we created induced pluripotent stem cells (iPSCs) from two...
Highlights•Combining three high-throughput methods provides low-cost characterization of iPSCs•iPSC line heterogeneity is assessed by fluorescent cell barcoding flow cytometry•12-gene qPCR enables gene expression analysis in vitro differentiation potential•SNP arrays provide inexpensive high-resolution digital karyotypingSummaryReprogramming somatic cells to induced pluripotent stem (iPSCs) offers the possibility studying molecular mechanisms underlying human diseases types difficult extract...
Reliable approaches to identify stem cell mechanisms that mediate aggressive cancer could have great therapeutic value, based on the growing evidence of embryonic signatures in metastatic cancers. However, how best and target stem-like aberrantly acquired by cells has been challenging. We harnessed power reprogramming examine GRP78, a chaperone protein generally restricted endoplasmic reticulum normal tissues, but which is expressed surface human many types. discovered (1) GRP78 (sGRP78)...
Abstract The causal variants and genes underlying thousands of cardiac GWAS signals have yet to be identified. Here, we leverage spatiotemporal information on 966 RNA-seq samples perform an expression quantitative trait locus (eQTL) analysis detecting eQTLs considering both eGenes eIsoforms. We identify 2,578 associated with a specific developmental stage-, tissue- and/or cell type. Colocalization between eQTL five traits identified high posterior probabilities for being in 210 loci. Pulse...