A5060221466- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Microbial Community Ecology and Physiology
- Advanced Proteomics Techniques and Applications
- Bacterial biofilms and quorum sensing
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Microbial Inactivation Methods
- Gut microbiota and health
- Blood properties and coagulation
- Legume Nitrogen Fixing Symbiosis
- Bacterial Identification and Susceptibility Testing
- Iron Metabolism and Disorders
University of Amsterdam
2011-2021
The endospore is the dormant form of Bacillus subtilis and many other Firmicutes. By sporulation, these spore formers can survive very harsh physical chemical conditions. Yet, they need to go through germination return their growing form. inner membrane (IM) has been shown play an essential role in triggering initiation germination. In this study, we isolated IM bacterial spores, parallel with isolation vegetative cells. With use GeLC–MS/MS, over 900 proteins were identified from B....
Bacillus cereus, responsible for food poisoning, and Clostridium difficile, the causative agent of difficile-associated diarrhea (CDAD), are both spore-forming pathogens involved in spoilage, intoxication, other infections humans animals. The proteinaceous coat exosporium layers from spores important their resistance pathogenicity characteristics. additionally provides an ability to adhere surfaces eventually leading spore survival food. Thus, studying these identifying suitable protein...
Spores are of high interest to the food and health sectors because their extreme resistance harsh conditions, especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat than those under liquid medium condition. It also been more mature spore is, is its most likely mediated, at least in part, by progressive cross-linking coat proteins. The current study for first time assesses, proteomic level, effect two commonly used sporulation...
Species from the genus Bacillus have ability to form endospores, dormant cellular forms that are able survive heat and acid preservation techniques commonly used in food industry. Resistance characteristics of spores towards various environmental stresses part attributed their coat proteins. Previously, 70 proteins been assigned spore subtilis using SDS-PAGE, 2-DE gel approaches, protein localization studies genome-wide transcriptome studies. Here, we present a "gel-free" protocol is capable...
Bacterial endospores, the transmissible forms of pathogenic bacilli and clostridia, are heterogeneous multilayered structures composed proteins. These proteins protect spores against a variety stresses, thus helping spore survival, assist in germination, by interacting with environment to form vegetative cells. Owing complexity, insolubility, dynamic nature proteins, it has been difficult obtain their comprehensive protein profiles.The intact Bacillus subtilis, cereus, Peptoclostridium...
Bacillus subtilis spores can reactivate their metabolism through germination upon contact with germinants and develop into vegetative cells outgrowth. However, the mechanisms at basis of molecular machinery that triggers spore outgrowth processes are still largely unclear. To gain further insights these processes, transcriptome proteome changes occurring during conversion to were analyzed in present study. For each time point sampled, quantitatively monitored relative metabolically...
The resistance properties of the bacterial spores are partially due to spore surface proteins, ∼30% which said form an insoluble protein fraction. Previous research has also identified a group coat proteins affected by maturation, exhibit increased level interprotein cross-linking. However, and types cross-links involved, previously proposed based on indirect evidence, have yet be confirmed experimentally. To obtain more insight into structural basis proteinaceous component coat, we...
Clostridioides difficile-associated infection (CDI) is a health-care-associated caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to other. In vivo, spores need germinate into cells prior establishing an infection. Bile acids glycine, both available in sufficient amounts inside human host intestinal tract, serve efficient germinants for spores. It therefore, better understanding of virulence,...
To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses effect of synchronized initiation resistance, structures, germination behavior at single-spore level proteome. The results indicate that, in our set up, can generate a yield 70% within 8 h. procedure increases wet heat resistance thickness coat cortex layers, whilst delaying time to phase-darkening burst after addition germinant. proteome...
Abstract Bacillus subtilis forms highly resistant, metabolically inactive dormant spores upon nutrient limitation. These endospores pose challenges to the food and medical sectors. Spores reactivate their metabolism contact with germinants develop into vegetative cells. The activation of molecular machinery that triggers progress germination spore outgrowth is still unsettled. To gain further insight in processes, transcriptome proteome changeover during cells, was analysed. B. analysis...
Clostridioides difficile -associated infection (CDI) is a health-care-associated mainly transmitted via highly resistant endospores from one person to the other. In vivo , spores need germinate in cells prior establishing an infection. Bile acids and glycine, both available sufficient amounts inside human host intestinal tract, serve as efficient germinants for spores. It therefore, better understanding of virulence, crucial study cell spore states with respect their genetic, metabolic...