A5067229730- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Advanced Proteomics Techniques and Applications
- interferon and immune responses
- Human Pose and Action Recognition
- Malaria Research and Control
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Erythrocyte Function and Pathophysiology
- Connexins and lens biology
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Gait Recognition and Analysis
- Plant-Microbe Interactions and Immunity
- Fatty Acid Research and Health
- Video Surveillance and Tracking Methods
- Photosynthetic Processes and Mechanisms
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Fungal and yeast genetics research
- Toxoplasma gondii Research Studies
- Antimicrobial Peptides and Activities
- Advanced Neural Network Applications
- Single-cell and spatial transcriptomics
Beijing Proteome Research Center
2022-2025
Nanchang University
2022-2024
Fujian Medical University
2024
State Key Laboratory of Proteomics
2024
University of Amsterdam
2018-2024
Jiangxi Agricultural University
2024
First Affiliated Hospital of Nanchang University
2022
Jilin University
2014-2018
University of Minnesota Medical Center
1992-1993
Bacterial endospores (spores) are among the most resistant living forms on earth. Spores of Bacillus subtilis A163 show extremely high resistance to wet heat compared spores laboratory strains. In this study, we found that B. were indeed very and released dipicolinic acid (DPA) slowly during treatment. We also determined proteome vegetative cells differences in these proteomes from those strain PY79, which much less resistant. This proteomic characterization identified 2011 proteins 1901...
Abstract Background Cowpea wilt is a harmful disease caused by Fusarium oxysporum , leading to substantial losses in cowpea production. Melatonin reportedly regulates plant immunity pathogens; however the specific regulatory mechanism underlying protective effect of melatonin pretreated against remains known. Accordingly, study sought evaluate changes physiological and biochemical indices following treated facilitate resistance elucidate associated molecular using weighted gene coexpression...
Previously, we reported a rat S1 protein that is antigenically related to statin, nonproliferating cell-specific marker; however, it shares high homology with the known human elongation factor-1 alpha (EF-1 alpha). To differentiate from EF-1 and study their respective regulation for expression, cDNA clone was isolated characterized. The nucleotide deduced amino acid sequences of this partial are compared mouse as well S1. Both messages were detected in brain by alpha- or S1-specific probes....
Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome dynamics during sporulation, high-resolution time-lapse proteomics was performed in a cell population that induced sporulate synchronously. Here, we are first comprehensively changeover of regulatory proteins, coat and other proteins involved spore biogenesis. Protein co-expression analysis revealed four co-expressed modules (termed blue, brown, green, yellow). Modules brown green...
Toxoplasma gondii is an obligatory intracellular apicomplexan parasite which exploits host cell surface components in invasion and parasitization. Sulfated glycans such as heparin heparan sulfate have been reported to inhibit by T. other parasites Plasmodium falciparum . The aim of this study was investigate the heparin‐binding proteome parasite‐derived were affinity‐purified on moiety followed MS fingerprinting proteins. proteins P. compared based functionality affinity heparin. Among...
Erythrocyte invasion by merozoites is an essential step in Plasmodium falciparum infection and leads to subsequent disease pathology. Proteins both on the merozoite surface secreted from apical organelles (micronemes, rhoptries dense granules) mediate of erythrocytes; some molecules have been regarded as targets development anti-malaria vaccine. Recently, a subgroup rhoptry neck proteins (PfRON2, PfRON4 PfRON5) associated with microneme protein membrane antigen AMA1 has described components...
Abstract Adamantinomatous craniopharyngioma (ACP) is a clinically aggressive tumor without effective treatment method. Previous studies proposed paracrine tumorigenesis model, in which oncogenic β-catenin induces senescence pituitary stem cells and the senescent lead formation of tumors through secretion pro-tumorigenic factors. However, there lacks characterization on ACPs. Here, we profiled 12 ACPs with single-cell RNA TCR-sequencing to elucidate cellular atlas 3 them were also subject...
The early diagnosis of hepatocellular carcinoma (HCC) has not been clinically elucidated, leading to an increased mortality rate in patients with HCC. HCC is a systemic disease related disorders blood homeostasis, and the association between red cells (RBCs) tumorigenesis remains elusive. We performed data-independent acquisition proteomic analyses 72 clinical RBC samples, including (n = 30), liver cirrhosis (LC, n 17), healthy controls 25), characterized relevance RBCs observed dynamic...
To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses effect of synchronized initiation resistance, structures, germination behavior at single-spore level proteome. The results indicate that, in our set up, can generate a yield 70% within 8 h. procedure increases wet heat resistance thickness coat cortex layers, whilst delaying time to phase-darkening burst after addition germinant. proteome...
Plasmodium falciparum is the most virulent parasite of five species that cause human malaria, and biological analysis critical for development novel strategies disease control. DNA endonucleases are important maintaining activity, gene stability interaction with host immune systems. In this study, ten sequences were found in genome P. 3D7 clone, seven them predicted to contain an endonuclease/exonuclease/phosphatase (IPR005135) domain which plays role catalytic activity. The systematically...
The inhalation of zinc chloride (ZnCl2) smoke is one common resources lung injury, potentially resulting in severe pulmonary complications and even mortality. influence ZnCl2 on lysine succinylation (Ksucc) the lungs remains uncertain. In this study, we used a mouse model to perform global proteomic succinylome analyses. A total 6781 Ksucc sites were identified lungs, with injured demonstrating reduction approximately 2000 sites, 91 proteins exhibiting at least five differences number sites....
Abstract In response to extreme conditions, Bacillus subtilis generates highly resilient spores characterized by a unique multilayered structure. This confers resistance against various chemicals and enzymes yet adding complexity the analysis of spore proteome. As first step in bottom-up proteomics, sample preparation poses significant challenge. We assessed how an optimized protocol for easy extraction digestion (SPEED) performed compared previously established methods “One-pot” (OP)...
Abstract Linear ubiquitination catalyzed by HOIL-1-interacting protein (HOIP), the key component of linear assembly complex, plays fundamental roles in tissue homeostasis executing domain-specific regulatory functions. However, a proteome-wide analysis interactome HOIP across tissues is lacking. Here, we present comprehensive mass spectrometry-based profiling four domains nine mouse tissues. The interaction dataset provides high-quality resource with an average approximately 90 interactors...
Long-term exposure to a microgravity environment leads structural and functional changes in hearts of astronauts. Although several studies have reported mechanisms cardiac damage under conditions, comprehensive research on at the protein level these is still lacking. In this study, proteomic analysis microgravity-exposed identified 156 differentially expressed proteins, ubiquitinomic 169 proteins with differential ubiquitination modifications. Integrated revealed that caused by transcription...
Abstract Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome dynamics during sporulation, high resolution time-lapse proteomics was performed in a cell population that induced sporulate synchronously. Here, we are first comprehensively changeover of regulatory proteins, coat proteins and other involved spore biogenesis. Protein co-expression analysis revealed four co-expressed modules (blue, brown, green yellow). Modules brown...