A5101936262- Neural dynamics and brain function
- Vestibular and auditory disorders
- Visual perception and processing mechanisms
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Olfactory and Sensory Function Studies
- Neural and Behavioral Psychology Studies
- Advanced MRI Techniques and Applications
- Medical Imaging Techniques and Applications
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- stochastic dynamics and bifurcation
- Neural Networks and Applications
- Memory and Neural Mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
- Functional Brain Connectivity Studies
- Advanced Vision and Imaging
- Photoreceptor and optogenetics research
- Face Recognition and Perception
- Evacuation and Crowd Dynamics
Howard Hughes Medical Institute
2024-2025
Janelia Research Campus
2024-2025
Princeton University
2016-2022
Neuroscience Institute
2016-2020
Advances in fluorescence microscopy enable monitoring larger brain areas in-vivo with finer time resolution. The resulting data rates require reproducible analysis pipelines that are reliable, fully automated, and scalable to datasets generated over the course of months. We present CaImAn, an open-source library for calcium imaging analysis. CaImAn provides automatic methods address problems common pre-processing, including motion correction, neural activity identification, registration...
The gradual accumulation of sensory evidence is a crucial component perceptual decision making, but its neural mechanisms are still poorly understood. Given the wide availability genetic and optical tools for mice, they can be useful model organisms study these phenomena; however, behavioral largely lacking. Here, we describe new evidence-accumulation task head-fixed mice navigating in virtual reality (VR) environment. As navigate down stem T-maze, see brief pulses visual on either side,...
To make successful evidence-based decisions, the brain must rapidly and accurately transform sensory inputs into specific goal-directed behaviors. Most experimental work on this subject has focused forebrain mechanisms. Using a novel evidence-accumulation task for mice, we performed recording perturbation studies of crus I lateral posterior cerebellum, which communicates bidirectionally with numerous regions. Cerebellar inactivation led to reduction in fraction correct trials. two-photon...
Recent work has highlighted that many types of variables are represented in each neocortical area. How can these neural representations be organized together without interference and coherently maintained/updated through time? We recorded from excitatory populations posterior cortices as mice performed a complex, dynamic task involving multiple interrelated variables. The encoding implied highly correlated were by less-correlated population modes, while pairs neurons exhibited spectrum...
Significance Rapid detection and processing of stimulus novelty are key elements adaptive behavior. Predictive coding theories postulate that novel stimuli should be encoded differently from familiar stimuli. Here, we show the majority neurons in layer 2/3 mouse primary visual cortex exhibit a significant excess response to The distinction between images developed rapidly, requiring only few repeated presentations. We this phenomenon can described by model cascading adaptation. This...
Abstract To explore theories of predictive coding, we presented mice with repeated sequences images novel sparsely substituted. Under these conditions, could be rapidly trained to lick in response a image, demonstrating high level performance on the first day testing. Using 2-photon calcium imaging record from layer 2/3 neurons primary visual cortex, found that evoked excess activity majority neurons. When new stimulus sequence was repeatedly presented, had similarly elevated for few...
The role of cerebellum in controlling eye movements is well established, but its contribution to more complex forms visual behavior has remained elusive. To study cerebellar activity during attention we recorded extracellular dentate nucleus (DN) neurons two non-human primates (NHPs). NHPs were trained read the direction indicated by a peripheral stimulus while maintaining fixation at center, and report cue performing saccadic movement into same following delay. We found that single-unit DN...
How does the brain internally represent a sequence of sensory information that jointly drives decision-making behavior? Studies perceptual have often assumed cortices provide noisy but otherwise veridical inputs to downstream processes accumulate and drive decisions. However, processing in even earliest can be systematically modified by various external internal contexts. We recorded from neuronal populations across posterior cortex as mice performed navigational task based on accumulating...
There is increased appreciation that dopamine (DA) neurons in the midbrain respond not only to reward 1 , 2 and reward-predicting cues 3 4 but also other variables such as distance 5, movements 6 – 11 behavioral choices 12–15. Based on these findings, a major open question how responses diverse are organized across population of DA neurons. In words, do individual multiplex multiple variables, or subsets specialized encoding specific variables? The reason this fundamental has been difficult...
Summary Studies of perceptual decision-making have often assumed that the main role sensory cortices is to provide input downstream processes accumulate and drive behavioral decisions. We performed a systematic comparison neural activity in primary visual (V1) secondary retrosplenial cortices, as mice task where they should pulsatile cues through time inform navigational decision. Even V1, only small fraction neurons had sensory-like responses cues. Instead, all areas were sequentially...
Abstract Advances in fluorescence microscopy enable monitoring larger brain areas in-vivo with finer time resolution. The resulting data rates require reproducible analysis pipelines that are reliable, fully automated, and scalable to datasets generated over the course of months. Here we present CaImAn, an open-source library for calcium imaging analysis. CaImAn provides automatic methods address problems common pre-processing, including motion correction, neural activity identification,...
Abstract Population recordings of calcium activity are a major source insight into neural function. Large dataset sizes often require automated methods, but automation can introduce errors that difficult to detect. Here we show automatic time course estimation sometimes lead significant misattribution errors, in which fluorescence is ascribed the wrong cell. Misattribution arises when shapes overlapping cells imperfectly defined, or entire processes not identified, and even be produced by...
Summary Recent work has highlighted that many types of variables are represented in each neocortical area. How can these neural representations be organized together without interference, and coherently maintained/updated through time? We recorded from large populations posterior cortices as mice performed a complex, dynamic task involving multiple interrelated variables. The encoding implied correlated were by uncorrelated neural-population modes, while pairs neurons exhibited variety...
Abstract Two-photon laser scanning microscopy of calcium dynamics using fluorescent indicators is a widely used imaging method for large scale recording neural activity in vivo. Here we introduce volumetric Imaging Neurons Stereoscopy (vTwINS), that employs an elongated, V-shaped point spread function to image 3D brain volume. Single neurons project spatially displaced pairs the resulting 2D image, and separation distance between images proportional depth To demix fluorescence time series...
ABSTRACT The gradual accumulation of sensory evidence is a crucial component perceptual decision making, but its neural mechanisms are still poorly understood. Given the wide availability genetic and optical tools for mice, they can be useful model organisms study these phenomena; however, behavioral largely lacking. Here, we describe new evidence-accumulation task head-fixed mice navigating in virtual reality environment. As navigate down stem T-maze, see brief pulses visual on either side,...
Abstract The role of cerebellum in controlling eye movements is well established, but its contribution to more complex forms visual behavior has remained elusive. To study cerebellar activity during attention we recorded extracellular dentate nucleus (DN) neurons two non-human primates (NHPs). NHPs were trained read the direction indicated by a peripheral stimulus while maintaining fixation at center, and report cue performing saccadic movement into same following delay. We found that single...
The role of cerebellum in controlling eye movements is well established, but its contribution to more complex forms visual behavior has remained elusive. To study cerebellar activity during attention we recorded extracellular dentate nucleus (DN) neurons two non-human primates (NHPs). NHPs were trained read the direction indicated by a peripheral stimulus while maintaining fixation at center, and report cue performing saccadic movement into same following delay. We found that single-unit DN...
The role of cerebellum in controlling eye movements is well established, but its contribution to more complex forms visual behavior has remained elusive. To study cerebellar activity during attention we recorded extracellular dentate nucleus (DN) neurons two non-human primates (NHPs). NHPs were trained read the direction indicated by a peripheral stimulus while maintaining fixation at center, and report cue performing saccadic movement into same following delay. We found that single unit DN...