A5031950517- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Plant Virus Research Studies
- Essential Oils and Antimicrobial Activity
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Regulation of Appetite and Obesity
- Tea Polyphenols and Effects
- COVID-19 Clinical Research Studies
- Drug-Induced Hepatotoxicity and Protection
- SARS-CoV-2 and COVID-19 Research
- Phytochemistry and Biological Activities
- Graphene and Nanomaterials Applications
- Biochemical Analysis and Sensing Techniques
- Insect Resistance and Genetics
- Adipose Tissue and Metabolism
- Fungal and yeast genetics research
- Cellular transport and secretion
- Bacteriophages and microbial interactions
- Biological and pharmacological studies of plants
- Bioactive natural compounds
- Adipokines, Inflammation, and Metabolic Diseases
- Carbon and Quantum Dots Applications
- Plant and Fungal Interactions Research
- Diverse Scientific Research Studies
Université de Lille
2007-2024
Inserm
2015-2024
Centre National de la Recherche Scientifique
2014-2024
Center for Infection and Immunity of Lille
2015-2024
Institut Pasteur de Lille
2014-2024
Centre Hospitalier Universitaire de Lille
2016-2024
Institut Pasteur
2006-2015
Université Lille Nord de France
2009-2015
Institut de Biologie de Lille
2006-2009
Laboratoire de Chimie
2000
Therapeutic options for the highly pathogenic human coronavirus (HCoV) infections are urgently needed. Anticoronavirus therapy is however challenging, as coronaviruses biologically diverse and rapidly mutating. In this work, antiviral activity of seven different carbon quantum dots (CQDs) treatment HCoV-229E was investigated. The first generation CQDs derived from hydrothermal carbonization ethylenediamine/citric acid precursors postmodified with boronic ligands. These nanostructures showed...
Here, we identify (−)-epigallocatechin - 3 -gallate (EGCG) as a new inhibitor of hepatitis C virus (HCV) entry. EGCG is flavonoid present in green tea extract belonging to the subclass catechins, which has many properties. Particularly, possesses antiviral activity and impairs cellular lipid metabolism. Because close links between HCV life cycle metabolism, postulated that may interfere with infection. We demonstrate concentration 50 μM inhibits infectivity by more than 90% at an early step...
Common single nucleotide polymorphisms (SNPs) in the ACDC adiponectin encoding gene have been associated with insulin resistance and type 2 diabetes several populations. Here, we investigate role of SNPs −11,377C>G, −11,391G>A, +45T>G, +276G>T 2,579 French Caucasians (1,229 morbidly obese 1,350 control subjects). We found an association between severe forms obesity −11,377C (odds ratio 1.23, P = 0.001) +276T (1.19, 0.006). Surprisingly, alternative alleles...
Despite the validation of direct-acting antivirals for hepatitis C treatment, discovery new compounds with different modes action may still be importance treatment special patient populations. We recently identified a natural molecule, epigallocatechin-3-gallate (EGCG), as an inhibitor virus (HCV) targeting viral particle. The aim this work was to discover higher anti-HCV activity than that EGCG and determine their mode action. Eight molecules structure similarity were selected. HCV JFH1 in...
Many studies on SARS-CoV-2 have been performed over short-time scale, but few focused the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis ultrastructure SARS-CoV-2-infected Early infection events were characterized by presence clusters single-membrane vesicles and stacks membrane containing nuclear pores called annulate lamellae (AL). A large network host cell-derived organelles transformed into virus factories was subsequently observed in As...
Abstract Older age is one of the strongest risk factors for severe COVID-19. In this study, we determined whether age-associated cellular senescence contributes to severity experimental Aged golden hamsters accumulate senescent cells in lungs, and senolytic drug ABT-263, a BCL-2 inhibitor, depletes these at baseline during SARS-CoV-2 infection. Relative young hamsters, aged had greater viral load acute phase infection displayed higher levels sequelae post-acute phase. Early treatment with...
The gene GAD2 encoding the glutamic acid decarboxylase enzyme (GAD65) is a positional candidate for obesity on Chromosome 10p11–12, susceptibility locus morbid in four independent ethnic populations. GAD65 catalyzes formation of γ-aminobutyric (GABA), which interacts with neuropeptide Y paraventricular nucleus to contribute stimulate food intake. A case-control study (575 morbidly obese and 646 control subjects) analyzing variants identified both protective haplotype, including most frequent...
Obesity is a major public health problem and often associated with type 2 diabetes mellitus, cardiovascular disease, metabolic syndrome. Leptin the crucial adipostatic hormone that controls food intake body weight through activation of specific leptin receptors (OB-R) in hypothalamic arcuate nucleus (ARC). However, most obese patients, high circulating levels fail to bring about loss. The prevention this "leptin resistance" goal for obesity research. We report here successful diet-induced...
The treatment of hepatitis C virus (HCV) infection by combination direct acting antivirals (DAA), with different mode action, has made substantial progress in the past few years. However, appearance resistance and high cost therapy is still an obstacle achievement therapy, more specifically developing countries. In this context, search for affordable new mechanisms action needed. Tea, after water, most popular drink worldwide. Polyphenols extracted from green tea have already shown anti-HCV...
Hepatitis C virus (HCV) entry involves binding to cell surface heparan sulfate (HS) structures. However, due the lipoprotein-like structure of HCV, exact contribution virion components this interaction remains controversial. Here, we investigated relative HCV envelope proteins and apolipoprotein E in HS-binding step. Deletion hypervariable region 1, a previously proposed be involved HS binding, did not alter HS, indicating that is context viral infection. Patient sera monoclonal antibodies...
The ORF YOL018c (TLG2) of Saccharomyces cerevisiae encodes a protein that belongs to the syntaxin family. proteins this family, t-SNAREs, are present on target organelles and thought participate in specific interaction between vesicles acceptor membranes intracellular membrane trafficking. TLG2 is not an essential gene, its deletion does cause defects secretory pathway. However, cells lacking vacuolar ATPase subunit Vma2p leads loss viability, suggesting Tlg2p involved endocytosis. In...
Recent emergence of direct-acting antivirals (DAAs) targeting hepatitis C virus (HCV) proteins has considerably enhanced the success antiviral therapy. However, appearance DAA-resistant-associated variants is a cause treatment failure, and high cost DAAs renders therapy not accessible in countries with inadequate medical infrastructures. Therefore, search for new inhibitors lower production should be pursued. In this context, crude extract Juncus maritimus Lam. was shown to exhibit activity...
ABSTRACT In Saccharomyces cerevisiae the FUR4 -encoded uracil permease catalyzes first step of pyrimidine salvage pathway. The availability has a negative regulatory effect upon its own transport. Uracil causes decrease in level permease, partly by decreasing mRNA promoter-independent fashion, probably increasing instability. entry also triggers more rapid degradation existing promoting high efficiency ubiquitination that signals internalization. A direct binding intracellular to is possibly...
Aminoquinolines and piperazines, linked or not, have been used successfully to treat malaria, some molecules of this family also exhibit antiviral properties. Here we tested several derivatives 4-aminoquinolines piperazines for their activity against hepatitis C virus (HCV). We screened 11 from three different families compounds, identified anti-HCV in cell culture six them. Of these, selected a compound (B5) that is currently ending clinical phase I evaluation neurodegenerative diseases. In...
In spite of the high variability its sequence, hepatitis C virus (HCV) envelope glycoprotein E2 contains several conserved regions. this study, we explored structural and functional features highly segment from amino acid (aa) 502 to 520, which had been proposed as a fusion peptide shown strongly overlap potential neutralizing epitope. For purpose, used reverse genetics introduce point mutations within region, characterized phenotypes these mutants in light recently published structure E2....
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak has highlighted the need for broad-spectrum antivirals against coronaviruses (CoVs). Here, pheophorbide a (Pba) was identified as highly active antiviral molecule human CoV-229E after bioguided fractionation of plant extracts.
GBF1 is a host factor required for hepatitis C virus (HCV) replication. functions as guanine nucleotide exchange G-proteins of the Arf family, which regulate membrane dynamics in early secretory pathway and metabolism cytoplasmic lipid droplets. Here we established that Arf-guanine activity critical its function HCV replication, indicating it promotes viral replication by activating one or more family members. involvement was confirmed with use two dominant negative Arf1 mutants. However,...
Abstract Coronavirus disease 2019 (COVID-19, caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2)) is primarily a illness. However, various extrapulmonary manifestations have been reported in patients with forms of COVID-19. Notably, SARS-CoV-2 was shown to directly trigger white adipose tissue (WAT) dysfunction, which turn drives insulin resistance, dyslipidemia, and other adverse outcomes Although advanced age the greatest risk factor for COVID-19 severity, published data...
Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that best possible antigenic components are chosen stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based proposed here as rapid and versatile platform for conjugation, utilizing peptides different pathogenic strains making this strategy good candidate "ready-to-use" vaccine. Initiation an inflammatory reaction with resulting...