A5103149743- Anesthesia and Neurotoxicity Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Particle physics theoretical and experimental studies
- Allergic Rhinitis and Sensitization
- Quantum Chromodynamics and Particle Interactions
- Asthma and respiratory diseases
- Neuroscience and Neuropharmacology Research
- Caveolin-1 and cellular processes
- Cardiac Ischemia and Reperfusion
- High-Energy Particle Collisions Research
- Anesthesia and Sedative Agents
- Intensive Care Unit Cognitive Disorders
- Mitochondrial Function and Pathology
- Cardiac, Anesthesia and Surgical Outcomes
- Olfactory and Sensory Function Studies
- Respiratory and Cough-Related Research
- Neurological Disease Mechanisms and Treatments
- Hemoglobin structure and function
- Neurological Disorders and Treatments
- Neutrino Physics Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Signaling Pathways in Disease
- Thermal Regulation in Medicine
- Natural Antidiabetic Agents Studies
- Ion channel regulation and function
Sun Pharma (India)
2023-2025
AISECT University
2024
Indian Institute of Technology Indore
2023
Providence Health Care
2021-2023
Sun Pharma Advanced Research (India)
2023
B.J. Medical College
2023
VA San Diego Healthcare System
2013-2022
University of California, San Diego
2013-2022
Providence College
2022
Navsari Agricultural University
2022
A CONVENTIONAL premise of general anesthesia is that anesthetics produce a nontoxic and reversible state unconsciousness. However, recent evidence has indicated exposure neonatal animals to triggers widespread neurodegeneration, leading persistent memory learning abnormalities during adulthood.1–3Specifically, postnatal day 5–10 (PND5-10) rat pups combination isoflurane, nitrous oxide, midazolam resulted in substantial neurodegeneration the hippocampus neocortex1as well as impaired...
The effects of propofol, etomidate, midazolam, and fentanyl on motor evoked responses to transcranial stimulation (tc-MERs) were studied in five healthy human volunteers. Each subject, four separate sessions, received intravenous bolus doses propofol 2 mg.kg-1, etomidate 0.3 midazolam 0.05 3 micrograms.kg-1. Electrical tc-MERs (tce-MERs) elicited with anodal stimuli 500-700 V. Magnetic (tcmag-MERs) using a Cadwell MES-10 magnetic stimulator at maximum output. Compound muscle action...
The aged brain exhibits a loss in gray matter and decrease spines synaptic densities that may represent sequela for neurodegenerative diseases such as Alzheimer's. Membrane/lipid rafts (MLR), discrete regions of the plasmalemma enriched cholesterol, glycosphingolipids, sphingomyelin, are essential development stabilization synapses. Caveolin-1 (Cav-1), cholesterol binding protein organizes signaling components within MLR. It is unknown whether synapses dependent on an age-related Cav-1...
Background Dexmedetomidine reduces cerebral blood flow (CBF) in humans and animals. In animal investigations, metabolic rate (CMR) was unchanged. Therefore, the authors hypothesized that dexmedetomidine would cause a decrease CBF/CMR ratio with even further reduction by superimposed hyperventilation. This might be deleterious patients neurologic injuries. Methods Middle artery velocity (CBFV) recorded continuously six volunteers. CBFV, jugular bulb venous saturation (Sjvo2), CMR equivalent...
Background— Caveolae, lipid-rich microdomains of the sarcolemma, localize and enrich cardiac-protective signaling molecules. Caveolin-3 (Cav-3), dominant isoform in cardiac myocytes, is a determinant caveolar formation. We hypothesized that myocyte–specific overexpression Cav-3 would enhance formation caveolae augment protection vivo. Methods Results— Ischemic preconditioning vivo increased caveolae. Adenovirus for phosphorylation survival kinases myocytes. A transgenic mouse with (Cav-3 OE)...
Propofol exposure to neurons during synaptogenesis results in apoptosis, leading cognitive dysfunction adulthood. Previous work from our laboratory showed that isoflurane neurotoxicity occurs through p75 neurotrophin receptor (p75(NTR)) and subsequent cytoskeleton depolymerization. Given propofol both suppress neuronal activity, we hypothesized also induces apoptosis developing p75(NTR).Days vitro 5-7 were exposed (3 μM) for 6 h was assessed by cleaved caspase-3 (Cl-Csp3) immunoblot...
To determine the effect of age on quadriceps muscle blood flow (QMBF), leg vascular resistance (LVR), and maximum oxygen uptake (QVO2 max), a thermal dilution technique was used in conjunction with arterial venous femoral sampling six sedentary young (19.8 +/- 1.3 yr) old (66.5 2.1 males during incremental knee extensor exercise (KE). Young attained similar maximal KE work rate (WRmax) (young: 25.2 old: 24.1 4 W) QVO2 max 0.52 0.03 0.42 0.05 l/min). QMBF lower subjects by approximately 500...
Background Several investigations have shown that volatile anesthetics can reduce ischemic cerebral injury. In these studies, however, neurologic injury was evaluated only after a short recovery period. Recent data suggest caused by ischemia is dynamic process characterized continual neuronal loss for prolonged Whether isoflurane-mediated neuroprotection sustained longer period not known. The current study conducted to compare the effect of isoflurane on brain (2-day) and long (14-day)...
The present study was undertaken to examine the cerebral protective properties attributed isoflurane and at same time compare its effects with those of mild hypothermia (temperature reduction by 3 degrees C). Twenty-one fasted Wistar-Kyoto rats were assigned one three groups (n = 7); 1.3 MAC (end-tidal) isoflurane-normothermia (pericranial temperature 38.0 C), halothane-normothermia, halothane-hypothermia 35.0 C during ischemia). In each animal trachea intubated lungs mechanically...
ABSTRACT Caveolae, small invaginations in the plasma membrane, contain caveolins (Cav) that scaffold signaling molecules including tyrosine kinase Src. We tested hypothesis cardiac protection involves a caveolin‐dependent mechanism. used vitro and vivo models of ischemia‐reperfusion injury, electron microscopy (EM), transgenic mice, biochemical assays to address this hypothesis. found Cav‐1 mRNA protein were expressed mouse adult myocytes (ACM). The volatile anesthetic, isoflurane, protected...
We studied the feasibility of recording motor evoked responses to transcranial electrical stimulation (tce-MERs) during partial neuromuscular blockade (NMB). In 11 patients, compound muscle action potentials were recorded from tibialis anterior in response various levels vecuronium-induced NMB. The level NMB was assessed by accelerometry adductor pollicis after train-of-four ulnar nerve. potential also direct peroneal nerve (M-response) as an alternative means assessing degree all tce-MERs...
The mechanisms by which isoflurane injured the developing brain are not clear. Recent work has demonstrated that it is mediated in part activation of p75 neurotrophin receptor. This receptor activates RhoA, a small guanosine triphosphatase can depolymerize actin. It therefore conceivable inhibition RhoA or prevention cytoskeletal depolymerization might attenuate neurotoxicity. study was conducted to test these hypotheses using primary cultured neurons and hippocampal slice cultures from...
N-Methyl-D-aspartate (NMDA) receptor (NMDAR) activation and downstream signaling are important for neuronal function. Activation of prosurvival Src family kinases extracellular signal-regulated kinase (ERK) 1/2 is initiated by NMDAR activation, but the cellular organization these in relation to NMDARs not entirely clear. We hypothesized that caveolin-1 scaffolds coordinates protein complexes involved this necessary preconditioning, whereby protects neurons from subsequent ischemic cell...
We show here that the apposition of plasma membrane caveolae and mitochondria (first noted in electron micrographs >50 yr ago) caveolae-mitochondria interaction regulates adaptation to cellular stress by modulating structure function mitochondria. In C57Bl/6 mice engineered overexpress caveolin specifically cardiac myocytes (Cav-3 OE), localization increases rigidity (4.2%; P<0.05), tolerance calcium, respiratory (72% increase state 3 23% complex IV activity; while reducing stress-induced...
Decreased expression of prosurvival and progrowth-stimulatory pathways, in addition to an environment that inhibits neuronal growth, contribute the limited regenerative capacity central nervous system following injury or neurodegeneration. Membrane/lipid rafts, plasmalemmal microdomains enriched cholesterol, sphingolipids, protein caveolin (Cav) are essential for synaptic development/stabilization signaling. Cav-1 concentrates glutamate neurotrophin receptors kinases regulates cAMP...
Abstract Background Traumatic brain injury (TBI) enhances pro-inflammatory responses, neuronal loss and long-term behavioral deficits. Caveolins (Cavs) are regulators of glial survival signaling. Previously we showed that astrocyte microglial activation is increased in Cav-1 knock-out (KO) mice Cav-3 modulate morphology. We hypothesized Cavs may regulate cytokine production after TBI. Methods Controlled cortical impact (CCI) model TBI (3 m/second; 1.0 mm depth; parietal cortex) was performed...
Abstract Climate change significantly impacts the global hydrological cycle, leading to pronounced shifts in hydroclimatic extremes such as increased duration, occurrence, and intensity. Despite these significant changes, our understanding of risks resilience remains limited, particularly at catchment scale peninsular India. This study aims address this gap by examining 54 catchments from 1988 2011. We initially assess extreme precipitation discharge indices estimate design return levels...
In Brief Although isoflurane can reduce ischemic neuronal injury after short postischemic recovery intervals, this neuroprotective efficacy is not sustained. Neuronal apoptosis contribute to the gradual increase in infarct size ischemia. This suggests that isoflurane, although capable of reducing early death, may inhibit ischemia-induced apoptosis. We investigated effects on markers rats subjected focal Fasted Wistar-Kyoto were anesthetized with and randomly allocated awake (n = 40) or...
AN INVESTIGATION WAS performed to compare the cerebral protective properties of etomidate, isoflurane, and thiopental. In separate groups spontaneously hypertensive rats, or thiopental was administered achieve maintain burst-suppression electroencephalogram (3–5 bursts/min) for duration experiment. A fourth group received 1.2 minimal alveolar concentration halothane. All underwent 3 hours middle artery occlusion then 2 reperfusion. Thereafter, animals were killed volume injured brain...