A5033877203- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Synthesis and Biological Evaluation
- Quinazolinone synthesis and applications
- Synthesis and biological activity
- Tuberculosis Research and Epidemiology
- X-ray Diffraction in Crystallography
- Biochemical and Structural Characterization
- Mycobacterium research and diagnosis
- Cancer therapeutics and mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Crystallization and Solubility Studies
- Synthetic Organic Chemistry Methods
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Plant-Microbe Interactions and Immunity
- ATP Synthase and ATPases Research
- Plant Pathogenic Bacteria Studies
- Antimicrobial Peptides and Activities
- Chemical Reactions and Isotopes
- Polyamine Metabolism and Applications
- Genomics and Phylogenetic Studies
- Plant-based Medicinal Research
- Amino Acid Enzymes and Metabolism
Karolinska Institutet
2025
Perm State University
2021-2024
Institute of Ecology and Genetics of Microorganisms
2021-2024
Ural Branch of the Russian Academy of Sciences
2024
Russian Academy of Sciences
2020
Although a variety of protocols to isolate high-molecular-weight genomic DNA exist, the isolation and preservation ultra-high-molecular-weight sufficient quality length for error-free third-generation sequencing remains challenging. Inspired by in agarose plugs suitable be separated pulsed-field gel electrophoresis, we report construction an incubation chamber isolation. The is flanked semi-permeable polycarbonate membranes variable pore size selective diffusion compounds components, which...
The treatment of many bacterial and fungal infections remains a problem due to increasing antibiotic resistance biofilm formation by pathogens. In the present article, methodology for chemoselective synthesis 2-(1H-indol-3-yl)-1H-benzo[d]imidazole derivatives is presented. We report on antimicrobial activity synthesized 2-(1H-indol-3-yl)-1H-benzo[d]imidazoles with significant against Staphylococcus aureus ATCC 25923, 43300 (MRSA), Mycobacterium smegmatis (mc(2)155/ATCC 700084), Candida...
The treatment of many bacterial diseases remains a significant problem due to the increasing antibiotic resistance their infectious agents. Among others, this is related Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA) and Mycobacterium tuberculosis. In present article, we report on antibacterial compounds with activity against both MRSA. A straightforward approach 2-(1H-indol-3-yl)quinazolin-4(3H)-one analogues was developed. Their structural functional relationships...
Suppression of the stringent response is a promising strategy for treatment persistent bacterial infections. A novel class compounds having mechanism action based on alarmone synthetase inhibition and suppressing synthesis (p)ppGpp alarmones in bacteria may provide more effective latent infections resolve problems associated with persistence. Conventional antibiotics primarily act actively growing bacteria, but they are inactive against persister cells slowed metabolism. Alarmone inhibitors...
The synthesis of (p)ppGpp alarmones plays a vital role in the regulation metabolism cessation, growth rate control, virulence, bacterial persistence, and biofilm formation. RelA/SpoT homologs superfamily proteins are responsible for alarmone synthesis, including long bifunctional RSH small synthetases. This study employs enzyme kinetics dose-dependent inhibition methods to investigate specific mechanism action DMNP involving RelMsm RelZ proteins, which synthetases Mycolicibacterium smegmatis...
Bacterial persistence coupled with biofilm formation is directly associated failure of antibiotic treatment TB. We have now identified 4-(4,7-DiMethyl-1,2,3,4-tetrahydroNaphthalene-1-yl)Pentanoic acid (DMNP), a synthetic diterpene analogue, as lead compound capable inhibiting persister cell formation, eradicating biofilms and affecting sliding motility in Mycobacterium smegmatis. The activities DMNP were enhanced by knockout mutations either relMsm or relZ genes and, conversely, reduced...
Using the complex of methods including reporter gene fusions, quantitative reverse transcriptase PCR and knockout mutations participation stress response genes ( rpoS, rmf, relA и spoТ ) in persister cell, formation was revealed E. coli batch cultures during transition to stationary phase. The expression levels these are positively modulated by polyamines (putrescine, spermidine, cadaverine) a concentration-depended manner. So, effects accumulated phase cells targeted potentiation functions...
Abstract Bacterial intercellular communication mediated by small diffusible molecules, known as quorum sensing (QS), is a common mechanism for regulating bacterial colonisation strategies and survival. Influence on QS plant-derived molecules proposed strategy combating phytopathogens modulating their virulence. This work builds upon other studies that have revealed inhibitors extracted from oak bark ( Quercus sp.). It was found co-incubation of Pectobacterium carotovorum VKM-B-1247 with...
The treatment of bacterial infections with antibiotics is significantly complicated because the adaptive mechanisms employed by bacteria. Chronic and recurrent are often linked to persis-tence, biofilm formation, antibiotic tolerance. These processes result in reduced metabolic activity, rendering bacteria insensitive conventional that primarily target actively growing cells. stringent re-sponse, regulated (p)ppGpp alarmone molecules, serves as a stress adaptation mechanism. It conserved...