A5045300492- Liver Disease Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Ocular Oncology and Treatments
- Drug-Induced Hepatotoxicity and Protection
- Cancer Genomics and Diagnostics
- DNA and Nucleic Acid Chemistry
- Hedgehog Signaling Pathway Studies
- Liver physiology and pathology
- Cancer, Lipids, and Metabolism
- Gene expression and cancer classification
- Cancer-related Molecular Pathways
- Organ Transplantation Techniques and Outcomes
- RNA and protein synthesis mechanisms
- Transplantation: Methods and Outcomes
- MicroRNA in disease regulation
- Antibiotics Pharmacokinetics and Efficacy
- Gene Regulatory Network Analysis
- Corneal Surgery and Treatments
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Laser Applications in Dentistry and Medicine
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Bioactive Compounds and Antitumor Agents
Institute of Biochemistry and Physiology of Plants and Microorganisms
2020
Inova Fairfax Hospital
2017-2018
St. Jude Children's Research Hospital
2011-2012
Sanofi (United States)
2004
University of Colorado Boulder
1995
Institut Jacques Monod
1992
Université Paris Cité
1992
University of Liège
1990
Institute of Mathematical Problems of Biology
1990
Institute of Molecular Genetics
1990
Early T-cell precursor acute lymphoblastic leukaemia (ETP ALL) is an aggressive malignancy of unknown genetic basis. We performed whole-genome sequencing 12 ETP ALL cases and assessed the frequency identified somatic mutations in 94 cases. was characterized by activating genes regulating cytokine receptor RAS signalling (67% cases; NRAS, KRAS, FLT3, IL7R, JAK3, JAK1, SH2B3 BRAF), inactivating lesions disrupting haematopoietic development (58%; GATA3, ETV6, RUNX1, IKZF1 EP300)...
Retinoblastoma is an aggressive childhood cancer of the developing retina that initiated by biallelic loss RB1. Tumours progress very quickly following RB1 inactivation but underlying mechanism not known. Here we show retinoblastoma genome stable, multiple pathways can be epigenetically deregulated. To identify mutations cooperate with loss, performed whole-genome sequencing retinoblastomas. The overall mutational rate was low; only known gene mutated. We then evaluated role in stability and...
Retinoblastoma is a childhood cancer of the developing retina that begins in utero and diagnosed first years life. Biallelic RB1 gene inactivation initiating genetic lesion retinoblastoma. The p53 intact human retinoblastoma but pathway believed to be suppressed by increased expression MDM4 (MDMX) MDM2. Here we quantify MDM2 mRNA protein fetal retinae, primary retinoblastomas, cell lines several independent orthotopic xenografts. We found major antagonist expressed retina. also discovered...
Drugs with clinical adverse effects are compared in an ex vivo 3-dimensional multi-cellular human liver slice model. Functional markers of oxidative stress and mitochondrial function, glutathione GSH ATP levels, were affected by acetaminophen (APAP, 1 mM), diclofenac (DCF, mM) etomoxir (ETM, 100 μM). targeting mitochondria more than dantrolene (DTL, 10 μM) cyclosporin A (CSA, μM), while was methimazole (MMI, 500 terbinafine (TBF, carbamazepine (CBZ Oxidative genes TBF (18%), CBZ, APAP, ETM...
Gecko (Gene Expression: Computation and Knowledge Organization) is a complete, high-capacity centralized gene expression analysis system, developed in response to the needs of distributed user community.Based on client-server architecture, with repository typically many tens thousands Affymetrix scans, includes automatic processing pipelines for uploading data from remote sites, base, computational engine implementing approximately 50 different tools, client application. Among available...
A method for the identification and characterization of protein-DNA interactions is presented. We have developed an approach finding unknown multiple patterns that occur imperfectly in a set several sequences. The pattern may contain letters from nucleotide alphabet (A, C, G T) including ambiguous characters (A/C, A/G, A/T; A/C/G, etc.). This reveals weak DNA signals on unaligned fragments known to be functionally related assumes no prior information sequences' alignment. It determines...
High-resolution mapping of topol cleavages in the regions human DNA including oncogene c-Ha-ras and p53, has revealed three kinds cleavage sites: sites not affected by camptothecin; reinforced only presence camptothecin, which weaken camptothecin. Statistical analysis sequences certain nucleotide or dinucleotide preferences for groups studied. The camptothecin-reduced predominate upstream from point, whereas camptothecin-induced situation is reversed. influence camptothecin on induced two...
Journal Article Electrophoretic behavior of d(GGAAAAAAGG)n, d(CCAAAAAACC)n, and (CCAAAAAAGG)n, implications for a DNA bending model Get access R.A. Abagyan, Abagyan * To whom correspondence should be addressed at Unversité de Liege, Laboratoire Genie Genetique, Institut Chimie B6, B 4000 Sart Tilman, Belgium Search other works by this author on: Oxford Academic PubMed Google Scholar V.N. Mironov, Mironov B.K. Chernov, Chernov V.P. Chuprina, Chuprina 1Research Computer CenterUSSR Academy...
Human liver slice function was stressed by daily dosing of acetaminophen (APAP) or diclofenac (DCF) to investigate injury and repair. Initially, untreated human kidney slices were evaluated with the global U133A array assess extended culture conditions. Then, drug induced signals repair in exposed APAP DCF (1 mM) via specific gene expression arrays. In culture, remained differentiated indicated that pathways activated both tissues. Morphologically exhibited evidence regeneration, while did...
Introduction: Stage of hepatic fibrosis caused by collagen deposition is the only independent histologic predictor mortality. Emerging evidence suggests a role for miR dysregulation in pathogenesis NAFLD. In fact, specific miRs profiles are associated with Our aim was to use sera from NAFLD patients assess differential signature expression assessed percentage (%) deposition. Methods: We included 66 biopsy-proven [64% NASH, 61% fibrosis]. Each liver biopsy stained Sirius red and % quantified...
Problems of agrobacterial transformation maize by methods in planta and vitro are described. Experimental data on optimization regeneration from transformed embryogenic callus the KM line presented.
Представлены результаты использования низкоинтенсивного лазерного облучения в целях профилактики послеоперационных раневых осложнений при ампутации бедра у пациентов с острой и хронической артериальной ишемией. Даны ультразвукового исследования культи бедра. Проанализированы факторы риска развития осложнений.
Introduction: NASH is the progressive form of non-alcoholic fatty liver disease (NAFLD). Stage fibrosis in predicts mortality. Phosphoproteomic and MicroRNAs (miRs) technologies can elucidate pathways involved pathogenesis NASH-related fibrosis. Methods: The study included tissue, serum clinical data from patients with NAFLD. Hepatic phosphoproteins were measured using Reverse Phase Protein Microarrays (RPMA). Using sera specimen, HTG EdgeSeq sequencing technology was used to sequence ˜2200...