Specific target binding and stability in diverse biological media is of crucial importance for applications synthetic oligonucleotides as diagnostic therapeutic tools. So far, these issues have been addressed by chemical modification conjugation with a peptide, most often at the terminal position oligonucleotide. Herein, we first time systematically investigate influence internally attached short peptides on properties antisense oligonucleotides. We report synthesis internal double labeling...

Lipid nanoparticles (LNPs) constitute a facile and scalable approach for delivery of payloads to human cells. LNPs are relatively immunologically inert can be produced in cost effective manner. However, targeting across the blood-brain barrier (BBB) has proven challenging. In an effort target composed ionizable cationic lipid (DLin-MC3-DMA), cholesterol, phospholipid 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG...

Hypercholesterolemia is a condition that characterized by very high levels of cholesterol in the blood and major correlating factor with heart disease. Indeed, low-density lipoprotein (LDL) have been causally linked to development atherosclerotic cardiovascular disease (ASCVD). A method specifically reduce long-term, stable manner could prove therapeutically relevant. Cholesterol removed from LDL receptor (LDLR) liver. Others we discovered long non-coding RNA (lncRNA; BM450697) functions as...

There is currently an unmet need for reliable tools that allow direct detection and quantification of modifications in genomic DNA.

Antigen recognition by antibodies plays an important role in human biology and the development of diseases. This interaction provides a basis for multiple diagnostic assays is guide treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA upon to (anti-DNA) provide optical response. The described herein advantageous properties compared those currently available dyes making them valuable research clinical diagnostics. By studying...

Detection of nucleic acids is crucial to the study their basic properties and consequently applying this knowledge determination pathologies such as cancer. In work, our goal determine new trends for creating diagnostic tools cancer driver mutations. Herein, we a library natural modified oligonucleotide duplexes by combination optical theoretical methods. We report profound effect additives on duplexes, including an active crowder. Unpredictably inconsistent with DNA+LNA/RNA locked...

New approaches for genomic DNA/RNA detection are in high demand order to provide controls existing enzymatic technologies and create alternatives emerging applications. In particular, there is an unmet need rapid, reliable of short RNA regions which could open up new opportunities transcriptome analysis, virology, other fields. Herein, we report the first time a "click" chemistry approach oligonucleotide probe elongation as novel specifically detect viral sequence. We hybridized library...

Biomedical research and clinical work demand rapid reliable detection of disease-associated nucleic acids. Fluorescent oligonucleotides that bind precisely, sense target DNA or RNA, are useful tools for simple hybridization-based assays. Although a plethora oligonucleotide modifications reported in the literature, they often result poor coupling yields very expensive. We describe synthesis new bisalkyne butane-1,3-diol scaffold its efficient into sequences. hypothesized covalent attachment...

Abstract For decades the detection of nucleic acids and their interactions at low abundances has been a challenging task that thus far solved by enzymatic target amplification. In this work we aimed developing efficient tools for amplification‐free acid detection, which resulted in synthesis new fluorescent nanoparticles. Here, nanoparticles were made simple inexpensive radical emulsion polymerization butyl acrylate presence dyes additional functionalization reagents. This provided...

Oligonucleotide therapeutics, antisense oligonucleotides (ASOs) and short interfering RNA (siRNA) are synthetic nucleic acid molecules with a promising potential to treat wide range of diseases. Despite considerable progress in the field, development safe effective delivery systems that target organs tissues other than liver is challenging. While keeping possible off-target oligonucleotide interactions toxicity related chemical modifications mind, innovative solutions for targeted...

Synthetic oligonucleotides, their complexes and conjugates with other biomolecules represent valuable research tools therapeutic agents. In spite of growing applications in basic clinical science, only few studies have addressed the issue such compounds' stability biological media. Herein, we studied two therapeutically relevant oligonucleotide probes simulated biofluids; 21 nucleotide-long DNA/locked nucleic acid ON targeted toward cancer-associated BRAF V600E mutation, a longer DNA analog...

DNA and RNA strand exchange is a process of fundamental importance in biology. Herein, we used FRET-based assay to investigate, for the first time, stand kinetics natural DNA, RNA, locked nucleic acid (LNA)-modified sequences vitro PBS absence or presence molecular additives macromolecular crowders such as diethylene glycol dimethyl ether (deg), polyethylene (peg), polyvinylpyrrolidone (pvp). The results show that mediated by LNA-DNA oligonucleotide are different. Different further affect...

Lipid nanoparticles (LNPs) constitute a facile and scalable approach for delivery of payloads to human cells. LNPs are relatively immunologically inert can be produced in cost effective manner. However, targeting across the blood brain barrier (BBB) has proven challenging. In an effort target particular cell types as well generating that cross BBB, we developed assessed two approaches whereby BBB penetrating peptides Tat or T7, RNA aptamers targeted gp160 from HIV CCR5, HIV-1 co-receptor,...