A5019151769- Vector-borne infectious diseases
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Insect and Pesticide Research
- Parasitic Infections and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Dermatological diseases and infestations
- Insect Pest Control Strategies
- Insect symbiosis and bacterial influences
- Trypanosoma species research and implications
- Entomopathogenic Microorganisms in Pest Control
- Yersinia bacterium, plague, ectoparasites research
- Research on Leishmaniasis Studies
- Rabies epidemiology and control
- Glutathione Transferases and Polymorphisms
- Zoonotic diseases and public health
- Mycobacterium research and diagnosis
- Amoebic Infections and Treatments
- vaccines and immunoinformatics approaches
- Parasites and Host Interactions
- Coccidia and coccidiosis research
- Helminth infection and control
- Malaria Research and Control
University of Missouri
2013-2022
Missouri College
2018-2021
The Ohio State University
2000-2007
Harvard University
2002
Washington State University
1998-2001
Agricultural Research Service
1992-2000
United States Department of Agriculture
2000
University of Georgia
1997
Oklahoma State University
1989-1993
Stillwater (Canada)
1990
DNA heterogeneity among members of the genus Brucella was demonstrated with arbitrarily primed polymerase chain reaction (AP-PCR). Simple, reproducible genomic fingerprints from 25 different strains were generated five chosen primers, alone and in pairs, PCR. Reaction conditions optimized for each primer. Several segments amplified sample all primers. PCR products that are not shared act as polymorphic markers. Polymorphism apparent The can be distinguished according to banding patterns...
Sarcocystis neurona is the most important cause of a neurologic disease in horses, equine protozoal myeloencephalitis (EPM). The complete life cycle S. neurona, including description sarcocysts and intermediate hosts, has not been completed until now. Opossums (Didelphis spp.) are definitive horses other mammals aberrant hosts. In present study, laboratory-raised domestic cats (Felis domesticus) were fed sporocysts from intestine naturally infected opossum virginiana). Microscopic...
Trypanosomes were observed in a peripheral blood smear from 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so parasite identification was undertaken determine likely sources of the infection. Several morphological parameters trypanosomes similar those Trypanosoma evansi statistically different lewisi-like parasites naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA...
Abstract: A rapidly increasing stray animal population in Bangkok has caused concern regarding transmission of vector‐borne and zoonotic diseases. The purpose this study was to determine if animals are a potential reservoir Hepatozoon , genus tick‐borne parasites that received little attention Thailand. Blood samples were collected from companion near monasteries 42 metropolitan districts. Both dogs cats sampled 26 districts, alone 4 districts 12 Samples total 308 300 cats. Light microscopy...
ABSTRACT Gamma interferon (IFN-γ)-activated macrophages are believed to play a key role in resistance Babesia bovis through parasite suppression by macrophage secretory products. However, relatively little is known about interactions between this intraerythrocytic and the of its bovine host. In study, we examined vitro effect intact fractionated B. merozoites on nitric oxide (NO) production. presence IFN-γ, stimulated NO production, as indicated increased l -arginine-dependent nitrite (NO 2...
Doxycycline is the treatment of choice for canine monocytic ehrlichiosis (CME), a well-characterized disease and valuable model tick-borne zoonoses. Conflicting reports clearance Ehrlichia canis after with doxycycline suggested that phase during which initiated influences outcomes these treatments. The purpose this study was to evaluate efficacy 28-day regimen experimental E. infections from dogs treated three phases disease. Ten were inoculated blood carriers acute, subclinical, or chronic...
Sixteen of 22 omp-1 paralogs encoding 28-kDa-range immunodominant outer membrane proteins Ehrlichia chaffeensis were transcribed in blood monocytes dogs throughout a 56-day infection period. Only one paralog was by E. three developmental stages Amblyomma americanum ticks before or after transmission to naïve dogs.
Human granulocytic ehrlichiosis (HGE) is an emerging tick-borne zoonosis caused by a strain of Anaplasma phagocytophila called the HGE agent, obligatory intracellular bacterium. The agent expresses immunodominant 44-kDa outer membrane proteins (P44s) encoded multigene family. present study established experimental process for transmission from infected mice (a reservoir model) to nymphal Ixodes scapularis ticks biological vector) and subsequently horses patient adult ticks. Overall, total 20...
ABSTRACT A liquid culture followed by molecular confirmation was evaluated for potential to improve sensitivity and reduce time diagnosis of Mycobacterium avium subsp. paratuberculosis infection. Fecal samples from 240 animals Ohio farms were assessed presence M. using four different protocols: (i) sedimentation processing inoculation on Herrold's Egg Yolk media (HEYM) slants (monitored biweekly up 16 weeks), (ii) double centrifugation HEYM (iii) liquid-solid method modified 7H9 broth (8...
Molecules secreted from the tick salivary gland modulate vertebrate host immune response, thus representing potential targets for novel control measures. Tick serine protease inhibitor (Serpin) is one such molecule that may facilitate feeding, blood meal digestion and pathogen transmission. The objective of this study was to determine immunogenicity protection recombinant Rhipicephalus (Boophilus) microplus Serpin (rSerpin) in rabbits. Rabbits were injected with rSerpin, adjuvant or...
ABSTRACT Detection of vector-borne pathogens is necessary for investigation their association with vertebrate and invertebrate hosts. The ability to detect Ehrlichia spp. within individual experimentally infected ticks would be valuable studies evaluate the relative competence different vector species transmission scenarios. purpose this study was develop a sensitive PCR assay based on oligonucleotide sequences from unique canis gene, p30 , facilitate that require monitoring pathogen in...
ABSTRACT Ehrlichia canis , an obligatory intracellular bacterium of monocytes and macrophages, causes canine monocytic ehrlichiosis. E . immunodominant 30-kDa major outer membrane proteins are encoded by a polymorphic multigene family consisting more than 20 paralogs. In the present study, we analyzed mRNA expression 14 paralogs in experimentally infected dogs Rhipicephalus sanguineus ticks reverse transcription-PCR using gene-specific primers followed Southern blotting. Eleven out were...