A5076111953- Vector-borne infectious diseases
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Toxin Mechanisms and Immunotoxins
- Mosquito-borne diseases and control
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Rabies epidemiology and control
- Antibiotic Resistance in Bacteria
- Plant Pathogenic Bacteria Studies
- Dermatological diseases and infestations
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Heat shock proteins research
- Cell death mechanisms and regulation
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Protein Hydrolysis and Bioactive Peptides
- Leptospirosis research and findings
- S100 Proteins and Annexins
- Endoplasmic Reticulum Stress and Disease
- Plant and Fungal Interactions Research
University of Shizuoka
2015-2024
Food & Nutrition
2011-2022
The University of Melbourne
2022
Japanese Red Cross Society Wakayama Medical Center
2022
National Institute of Infectious Diseases
2018-2022
Shizuoka Institute of Science and Technology
2022
Peter Doherty Institute
2022
Ise Red Cross Hospital
2022
Jeju National University
2018
Hetao College
2018
Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other in Rickettsiales order. spp. display a unique large expansion immunodominant outer membrane proteins facilitating antigenic variation. All have diminished ability synthesize amino acids compared their...
ABSTRACT Several immunodominant major proteins ranging from 23 to 30 kDa were identified in the outer membrane fractions of Ehrlichia chaffeensis and canis . The N-terminal amino acid sequence a 28-kDa protein E. (one proteins) was determined. gene ( p28 ), almost full length, encoding cloned by PCR with primers designed based on consensus between C termini Cowdria ruminantium MAP-1 Anaplasma marginale MSP-4 proteins. overexpressed, antibody recombinant raised rabbit. serum patient infected...
Abstract We report Anaplasma phagocytophilum infection of Ixodes persulcatus and I. ovatus ticks in Japan. Unique p44/msp2 paralogs (and/or 16S rRNA genes) were detected tick tissues, salivary glands, spleens experimentally infected mice. These findings indicate the public health threat anaplasmosis
Abstract Angiogenesis is crucial for tumor growth and hematogenous metastasis. Specifically expressed functional protein molecules in angiogenic endothelial cells, especially on the plasma membrane, may be molecular targets antiangiogenic drugs drug delivery systems (DDS) cancer therapy. To discover such target molecules, we performed subcellular proteome analysis of human umbilical vein cells (HUVECs) treated with or without vascular factor (VEGF) using 2‐dimensional difference in‐gel...
To evaluate the diversity of extended-spectrum β-lactamases (ESBL) genes among food-producing animals, 48 isolates ESBL-producing Escherichia coli were obtained from rectal samples broilers, layers, beef cattle and pigs, at slaughterhouse level. ESBL-carrying E. isolated 60.0% individual broiler samples, 5.9% 12.5% 3% pigs. One Klebsiella pneumoniae was a broiler. The ESBL-positive broilers harbored various ESBL genes: bla (SHV-12), bla(CTX-M-2), bla(CTX-M-14), bla(CTX-M-15) bla(CTX-M-44)....
ABSTRACT Anaplasma ( Ehrlichia ) phagocytophila and chaffeensis , the etiologic agents of granulocytic monocytic ehrlichioses, respectively, are obligatory intracellular bacteria that cause febrile systemic illness in humans. We identified characterized clusters genes for a type IV secretion machinery these two bacteria, analyzed their gene expression cell culture mammalian hosts. Eight virB virD were found each bacterial genome, all transcribed culture. Although order orientation similar to...
There are several antigenic variants in Rickettsia tsutsugamushi, and a type-specific antigen (TSA) of 56-kilodaltons located on the rickettsial surface is responsible for variation. The primary structures protein two variants, Gilliam Karp, have been reported independently by us Stover et al. cloning sequencing corresponding genes (Ohashi, N., Nashimoto, H., Ikeda, Tamura, A. (1990) Gene (Amst.) 91, 119-122; Stover, C. K., Marana, D. P., Carter, J. M., Roe, B. A., Mardis, E., Oaks, E. V....
A 30-kDa major outer membrane protein of Ehrlichia canis, the agent canine ehrlichiosis, is antigen recognized by both naturally and experimentally infected dog sera. The cross-reacts with a serum against recombinant 28-kDa (rP28), one proteins gene (omp-1) family chaffeensis. Two DNA fragments E. canis were amplified PCR two primer pairs based on sequences chaffeensis omp-1 genes, cloned, sequenced. Each fragment contained partial canis. Genomic Southern blot analysis probes revealed...
A 44-kDa major outer membrane protein of the human granulocytic ehrlichiosis (HGE) agent is an immunodominant antigen in infection. gene encoding this was cloned and sequenced. Southern blot results revealed existence multigenes homologous to P44 genome HGE agent. The recombinant (rP44) expressed by using expression vector pET30a. reactivity affinity-purified rP44 evaluated Western immunoblot analysis dot immunoassay. showed that mouse anti-rP44 serum reacted with 44- 42-kDa proteins six...
Human granulocytic ehrlichiosis (HGE) is caused by infection with an obligatory intracellular bacterium, the HGE agent. We previously cloned a gene encoding agent 44-kDa major outer membrane protein and designated it p44. In this study, we (i) identified five different mRNAs that are transcribed from p44-homologous genes in cultivated HL-60 cells; (ii) corresponding to genomic DNA of agent; (iii) showed being expressed were not clustered genome; (iv) estimated minimum copy number genome 18;...
Polyacrylamide gel electrophoresis of lysates purified Rickettsia tsutsugamushi revealed as many 30 polypeptide bands, including major bands corresponding to molecular sizes 70, 60, 54 56, and 46 47 kilodaltons. Compared with the composition rickettsiae Gilliam, Karp, Kato strains a newly isolated Shimokoshi strain, in strain (54-56K) Karp (46-47K) migrated little faster slower, respectively, than polypeptides other strains. The largest was digestible by treatment intact trypsin variable...
Analyses of chemical composition in whole cells Rickettsia tsutsugamushi were performed and compared with those the other rickettsiae gram-negative bacteria. The results indicated that R. does not contain detectable amounts 3-deoxy-D-mannooctulosonic acid, heptose, muramic or glucosamine (less than 2, less 3, 3 nmol/mg, respectively). microorganism was found to four kinds fatty acids (16:0, 18:0, 18:1, 18:2), but hydroxy acids. Furthermore, analysis by sodium dodecyl sulfate-polyacrylamide...
Here, we describe for the first time prevalence and genetic properties of Bartonella organisms in wild rodents Japan. We captured 685 throughout Japan (in 12 prefectures) successfully isolated from 176 (isolation rate, 25.7%). Those isolates were all obtained suburban areas (rate, 51.8%), but no organism was animals city areas. Sequence analysis rpoB gltA revealed that classified into eight groups, comprising closely related to B. grahamii (A-I group), tribocorum elizabethae (B-J...
ABSTRACT Ehrlichia canis and E. chaffeensis are tick-borne obligatory intramonocytic ehrlichiae that cause febrile systemic illness in humans dogs, respectively. The current study analyzed the pleomorphic multigene family encoding approximately 30-kDa major outer membrane proteins (OMPs) of . Upstream from secA downstream hypothetical transcriptional regulator, 22 paralogs omp gene were found to be tandemly arranged except for one or two genes with opposite orientations a 28- 27-kb locus...
Abstract A total of 40 strains Orientia tsutsugamushi (34 isolates from patients and trombiculid mites in Japan, 6 prototype antigenic variants) were examined for classification based on the reactivities with type‐specific monoclonal antibodies indirect immunofluorescence tests, restriction fragment length polymorphism a polymerase chain reaction (PCR)‐amplified 56‐kilodalton protein gene. By these methods, several genotypic variants found among strains, classified into types further...
Abstract Nine isolates of Rickettsia tsutsugamushi were obtained from patients with Tsutsugamushi disease (scrub typhus) in Miyazaki Prefecture Kyushu. Immunological analyses these patients' sera and the performed by indirect immunofluorescence, immunoperoxidase or immunoblotting techniques. In analysis reactions prototype strains Gilliam, Karp, Kato isolates, two patients, including Kawasaki, showed similar profiles cross‐reaction recovered corresponding but reacted only weakly strains....
Abstract We retrospectively confirmed 2 cases of human Anaplasma phagocytophilum infection. Patient blood samples contained unique p44/msp2 for the pathogen, and antibodies bound to A. antigens propagated in THP-1 rather than HL60 cells. Unless both cell lines are used serodiagnosis rickettsiosis-like infections, granulocytic anaplasmosis could go undetected.
Polymerase chain reaction (PCR) was used to detect Rickettsia tsutsugamushi-specific DNA in clinical specimens. The primer pair for PCR designed from the nucleotide sequence of gene encoding 56-kDa antigen Gilliam strain. Theses primers led a 78-bp fragment by amplifying genomic DNAs five serovariants, i.e., Gilliam, Karp, Kato, Kawasaki, and Kuroki strains R. tsutsugamushi, also blood clots patients with scrub typhus, even at early stage onset disease. This indicates that this method is...
The Kuroki strain of Rickettsia tsutsugamushi, isolated from a patient in Kyushu, Japan, has major, type-specific antigenic polypeptide which is distinct the prototype strains size (58 kilodaltons), antigenicity, and its cleavage pattern with N-chlorosuccinimide. These results indicate that another type R. tsutsugamushi.
We found Rickettsia raoultii infection in 6/261 brucellosis-negative patients with fever of unknown origin brucellosis-endemic Inner Mongolia, China. further identified Hyalomma asiaticum ticks associated R. raoultii, H. marginatum aeschlimannii, and Dermacentor nuttalli both rickettsiae species the autonomous region.
ABSTRACT Ehrlichia chaffeensis and E. sennetsu are genetically divergent obligatory intracellular bacteria of human monocytes macrophages, the granulocytic ehrlichiosis (HGE) agent is an bacterium granulocytes. Infection with both , but not HGE agent, in acute monocytic leukemia cell line THP-1 almost completely inhibited by treatment deferoxamine, a cell-permeable iron chelator. Transferrin receptors (TfRs) accumulated on inclusions cells or promyelocytic HL-60. Reverse transcription-PCR...
Sixteen of 22 omp-1 paralogs encoding 28-kDa-range immunodominant outer membrane proteins Ehrlichia chaffeensis were transcribed in blood monocytes dogs throughout a 56-day infection period. Only one paralog was by E. three developmental stages Amblyomma americanum ticks before or after transmission to naïve dogs.
Anaplasma phagocytophilum, an obligate intracellular bacterium, is the aetiologic agent of human granulocytic anaplasmosis (HGA). A. phagocytophilum virB/D operons encoding type IV secretion system are expressed in cell culture and blood HGA patients. In present study, their expression across developmental cycle was investigated. We found that mRNA levels both virB9 virB6 were upregulated during infection neutrophils vitro. The antibody against recombinant VirB9 protein prepared immunogold...
Human granulocytic ehrlichiosis (HGE) is an emerging tick-borne zoonosis caused by a strain of Anaplasma phagocytophila called the HGE agent, obligatory intracellular bacterium. The agent expresses immunodominant 44-kDa outer membrane proteins (P44s) encoded multigene family. present study established experimental process for transmission from infected mice (a reservoir model) to nymphal Ixodes scapularis ticks biological vector) and subsequently horses patient adult ticks. Overall, total 20...