A5003022407- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- Colorectal Cancer Treatments and Studies
- Pancreatic and Hepatic Oncology Research
- MicroRNA in disease regulation
- Advanced Fluorescence Microscopy Techniques
- Ferroptosis and cancer prognosis
- Animal and Plant Science Education
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Diverse Educational Innovations Studies
- Acute Myeloid Leukemia Research
Nashville Oncology Associates
2025
Vanderbilt University Medical Center
2025
Dana-Farber Cancer Institute
2020-2024
Division of Undergraduate Education
2017
Metastasis causes most cancer deaths and reflects transitions from primary tumor escape to seeding growth at metastatic sites. Epithelial-to-mesenchymal transition (EMT) is important early in metastasis enable cells detach neighboring cells, become migratory, the tumor. While different phases of expose variable nutrient environments demands, metabolic requirements plasticity each step are uncertain. Here we show that EMT stimulated by disrupted oxidative metabolism. Using Renal Cell...
As mass cytometry (MC) is implemented in clinical settings, the need for robust, validated protocols that reduce batch effects between samples becomes increasingly important. Here, we present a streamlined MC workflow high-throughput staining generates reproducible data up to 80 single experiment by combining reference sample spike-in and palladium-based mass-tag cell barcoding. Although labor intensive, this decreases experimental variables thus reduces technical error mitigates effects.
Background The combination of antiangiogenic agents with immune checkpoint inhibitors could potentially overcome suppression driven by tumor angiogenesis. We report results from a phase IB study ziv-aflibercept plus pembrolizumab in patients advanced solid tumors. Methods This is multicenter dose-escalation the (at 2–4 mg/kg) 2 administered intravenously every weeks expansion cohorts programmed cell death protein 1 (PD-1)/programmed death-ligand 1(PD-L1)-naïve melanoma, renal carcinoma...
We developed a comprehensive method for functional assessment of the changes in immune populations and killing activity peripheral blood mononuclear cells after cocultures with cancer using mass cytometry. In this study, 43-marker cytometry panel was applied to coculture from healthy donors' diverse cell lines. DNA content combined classical CD45 surface staining used as gating parameters (CD45high/DNAlow) hematological (CD45low/DNAhigh) solid lines (CD45neg/DNAhigh). This strategy allows...
Nivolumab plus ipilimumab (aCTLA-4/aPD-1) combination therapy has significantly improved clinical outcomes in patients with metastatic melanoma, 50%-60% of responding to treatment, but predictors response are poorly characterized. We hypothesized that circulating cytokines and peripheral white blood cells may predict evaluated 15 complete counts (CBC differentials) from 89 advanced melanoma treated three points time: pre-treatment, one month approximately months after starting therapy....
With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) trials is needed. We present in-plate staining up to 80 samples, laboratories all expertise levels, aimed generate reproducible datasets large cohorts. In this protocol, we provide details on requirements obtain meaningful results, spanning from sample quality, barcoding, batch-freezing stained samples.
Abstract Background:aCTLA-4 and aPD-1 combination therapy has significantly improved clinical outcomes in patients with metastatic melanoma, 50%-60% of responding to treatment, but predictors response treatment induced immunological effects are poorly characterized. Identifying circulating immune populations as biomarkers could reveal crucial insights into the mechanisms driving success. Methods:Using unbiased single-cell profiling (with cytometry by Time-Of-Flight, CyTOF), we characterized...
Background Angiogenic factors play a role in regulating immune suppression the tumor microenvironment and driving resistance to checkpoint inhibitor therapy. 1 Ziv-aflibercept is soluble decoy receptor that ”traps” endogenous vascular endothelial growth factor (VEGF) with 100-fold increased binding affinity compared Bevacizumab. 2 The combination of ziv-aflibercept either cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) or programmed cell death (PD-1) blockade has shown promising...
Phenotypic and functional assessment of Natural Killer (NK) cell activity against cancer cells in a clinical setting requires the integration labor-intensive multicolor flow cytometry, chromium (Cr) release assays, CD107a degranulation assays. To merge these into single high-throughput assay, we developed method for profiling NK-cancer interactions simultaneous target killing using mass cytometry. In this study, multiparameter 43-marker cytometry panel was applied to co-culture immune from...