A5060112905- Thyroid Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ion Transport and Channel Regulation
- Physiological and biochemical adaptations
- CRISPR and Genetic Engineering
- Adventure Sports and Sensation Seeking
- Congenital heart defects research
- Animal Genetics and Reproduction
- Developmental Biology and Gene Regulation
- Inflammatory Bowel Disease
- Neuroendocrine regulation and behavior
- Renal and related cancers
- Psychosocial Factors Impacting Youth
- Polyamine Metabolism and Applications
- Fatty Acid Research and Health
- Protein Hydrolysis and Bioactive Peptides
- Diverse Approaches in Healthcare and Education Studies
- Technology and Data Analysis
- Outdoor and Experiential Education
- Gastric Cancer Management and Outcomes
- Retirement, Disability, and Employment
- Behavioral Health and Interventions
- Education and Learning Interventions
- Youth Development and Social Support
Center for Cancer Research
2025
Nara City Hospital
2021-2025
Cancer Institute (WIA)
2025
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2017-2024
National Institutes of Health
2016-2024
National Institute for Basic Biology
2022-2024
Showa University Koto Toyosu Hospital
2015-2024
Showa University
2015-2024
Nippon Medical School
2023-2024
Kinjo Gakuin University
2024
Thyroid hormone (T3) receptors (TRs) mediate the effects of T3 on organ metabolism and animal development. There are two TR genes, TRα TRβ, in all vertebrates. During development, expression is activated earlier than zygotic synthesis secretion into plasma, implicating a developmental role both presence absence T3. Using T3-dependent amphibian metamorphosis as model, we previously proposed dual-function model for TRs, particular TRα, during That is, unliganded represses T3-inducible genes...
Dietary ω3 fatty acids have important health benefits and exert their potent bioactivity through conversion to lipid mediators. Here, we demonstrate that microbiota play an essential role in the body's use of dietary lipids for control inflammatory diseases. We found amounts 10-hydroxy-cis-12-cis-15-octadecadienoic acid (αHYA) 10-oxo-cis-12-cis-15-octadecadienoic (αKetoA) increased feces serum specific-pathogen-free, but not germ-free, mice when they were maintained on a linseed oil diet,...
Metastasis causes most cancer deaths and reflects transitions from primary tumor escape to seeding growth at metastatic sites. Epithelial-to-mesenchymal transition (EMT) is important early in metastasis enable cells detach neighboring cells, become migratory, the tumor. While different phases of expose variable nutrient environments demands, metabolic requirements plasticity each step are uncertain. Here we show that EMT stimulated by disrupted oxidative metabolism. Using Renal Cell...
Abstract Background The PROFILE study1) highlighted the importance of top-down treatment with Advanced Therapy (AT) in Crohn’s disease. In ulcerative colitis (UC), corticosteroid (CS) cascade remains crucial for selecting AT candidates. CS efficacy decreases repeated use, affecting therapeutic outcomes GMA and carotegrast. We hypothesized that total dose impacts subsequent examined this relationship to optimize induction timing. Methods retrospectively reviewed medical records UC patients...
Abstract Background Thyroid hormone (T3) is critical for development in all vertebrates. The mechanism underlying T3 effect has been difficult to study due the uterus-enclosed nature of mammalian embryos. Anuran metamorphosis, which dependent on but independent maternal influence, an excellent model roles and its receptors (TRs) during vertebrate development. We others have reported various effects TR knockout ( TRα TRβ ) Xenopus tropicalis However, these studies were largely focused...
Abstract Atherosclerosis is a chronic inflammatory disease associated with macrophage aggregate and transformation into foam cells. In this study, we sought to investigate the impact of dietary intake ω3 fatty acid on development atherosclerosis, demonstrate mechanism action by identifying anti-inflammatory lipid metabolite. Mice were exposed high-fat diet (HFD) supplemented either conventional soybean oil or α-linolenic acid-rich linseed oil. We found that as mice became obese they also...
Background: Thyroid hormone (triiodothyronine [T3]) is essential for the development throughout vertebrates. Anuran metamorphosis mimics mammalian postembryonic development, a period around birth when plasma T3 level peaks and many organs/tissues mature into their adult forms. Compared with uterus-enclosed embryos, tadpoles can be easily manipulated to study roles of receptors (TRs) in tissue remodeling organ development. We others have previously knocked out TRα or TRβ diploid anuran...
In amniote limbs, Fibroblast Growth Factor 10 (FGF10) is essential for limb development, but whether this function broadly conserved in tetrapods and/or involved adult regeneration remains unknown. To tackle question, we established Fgf10 mutant lines the newt Pleurodeles waltl which has amazing regenerative ability. While forelimbs develop normally, hindlimbs fail to and downregulate FGF target genes. Despite these developmental defects, mutants were able regenerate normal rather than...
Background: There are two highly conserved thyroid hormone (triiodothyronine [T3]) receptor (TR) genes, TRα and TRβ, in all vertebrates, the expression of but not TRβ is activated earlier than T3 synthesis during development. In human, high levels present several months around birth, deficiency this period causes severe developmental abnormalities including skeletal intestinal defects. It is, however, difficult to study mammals as embryos neonates depend on maternal supply nutrients for...
Vertebrate postembryonic development is regulated by thyroid hormone (T3). Of particular interest anuran metamorphosis, which offers several unique advantages for studying the role of T3 and its two nuclear receptor genes, TRα TRβ, during development. We have recently generated TR double knockout (TRDKO) Xenopus tropicalis animals reported that essential completion metamorphosis. Furthermore, TRDKO tadpoles are stalled at climax metamorphosis before eventual death. Here we show intestine...
Abstract Thyroid hormone (T3) regulates adult intestine development through T3 receptors (TRs). It is difficult to study TR function during postembryonic intestinal maturation in mammals due maternal influence. We chose remodeling Xenopus tropicalis metamorphosis as a model organ development. By using ChIP (chromatin immunoprecipitation)-Seq, we identified over 3000 TR-bound genes the of premetamorphic wild type or TRα (the major expressed premetamorphosis)-knockout tadpoles. Surprisingly,...
Abstract In this study, we fabricate MXene transparent conductive films via a transfer process. The results show that the transferred using titanium carbide have ca. 100 times higher electrical conductivity than conventional spray-coated samples. Transparency and are easily controlled by changing amount of materials. scanning electron microscopy observations reveal smooth uniform flake network. closer interlayer distance flakes when compared to sample, which enables superior conductivity, is...
Animal regeneration is the natural process of replacing or restoring damaged missing cells, tissues, organs, and even entire body to full function. Studies in mammals have revealed that many organs lose regenerative ability soon after birth when thyroid hormone (T3) level high. This suggests T3 play an important role organ regeneration. Intriguingly, plasma peaks during amphibian metamorphosis, which very similar postembryonic development humans. In addition, such as heart tail, also their...
Background: Thyroid hormone (triiodothyronine [T3]) is essential for development and organ metabolism in all vertebrates. T3 has both genomic nongenomic effects on target cells. While much been learnt its via receptors (TRs) vertebrate development, mostly through TR-knockout TR-knockin studies, little known about the of gene expression animals absence TR. We have studying Xenopus metamorphosis as a model mammalian postembryonic period around birth when plasma level peaks many organs/tissues...
Thyroid hormone (T3) receptors (TRs) mediate T3 effects on vertebrate development. We have studied Xenopus tropicalis metamorphosis as a model for postembryonic human development and demonstrated that TRα knockout induces precocious hind limb To reveal the molecular pathways regulated by during development, we performed chromatin immunoprecipitation- RNA-sequencing of premetamorphic wild type tadpoles, identified over 700 TR-bound genes upregulated treatment in but not tadpoles....
Until now, it was believed that only one form of arginine vasotocin (AVT)-regulated aquaporin (AQP) existed to control water absorption from the ventral skin semiaquatic anuran amphibians, eg, AQP-rj3(a) in Rana japonica. In present study, we have identified a novel skin-type AQP, AQP-rj3b, R. japonica by cDNA cloning. The oocyte swelling assay confirmed AQP-rj3b can facilitate permeability. Both AQP-rj3a and were expressed abundantly hindlimb weakly pelvic skin. For skin, permeability...
This study describes the concise exfoliation of multilayer Ti3C2T x MXene containing residual aluminum atoms. Treatment with tetramethylammonium base in a co-solvent tetrahydrofuran and H2O produced single-layer , which was confirmed via atomic force microscopy observations, an electrical conductivity 100+ times that prepared under previously reported conditions. The scanning electron X-ray diffraction measurements showed exfoliated MXenes were reconstructed to assembled large-domain layered...
Two types of aquaporin 5 (AQP5) genes ( aqp-xt5a and aqp-xt5b) were identified in the genome Xenopus tropicalis by synteny comparison molecular phylogenetic analysis. When frogs water, AQP-xt5a mRNA was expressed skin urinary bladder. The expression significantly increased dehydrated frogs. AQP-xt5b also detected response to dehydration. Additionally, began be slightly lung stomach after For pelvic hydrated frogs, immunofluorescence staining localized cytoplasm secretory cells granular...
For adult anuran amphibians, the kidney and urinary bladder play important osmoregulatory roles through water reabsorption. In present study, we have examined ontogenetic expression of aquaporins, i.e., AQP2, AQPamU (AQP6ub, AQPa2U), AQP3, in these organs using Japanese tree frog,