A5027651090- Library Science and Information Literacy
- Protease and Inhibitor Mechanisms
- Library Science and Administration
- Virus-based gene therapy research
- Peptidase Inhibition and Analysis
- Irish and British Studies
- Web and Library Services
- RNA and protein synthesis mechanisms
- Political and Economic history of UK and US
- Labor Movements and Unions
- Blood Coagulation and Thrombosis Mechanisms
- Venomous Animal Envenomation and Studies
- Cardiac, Anesthesia and Surgical Outcomes
- Electoral Systems and Political Participation
- Social Policy and Reform Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Media Influence and Politics
- Animal Genetics and Reproduction
- Recreation, Leisure, Wilderness Management
- Cancer Research and Treatments
- Dental Education, Practice, Research
- Global Health Workforce Issues
- Liver Diseases and Immunity
- Biochemical and Structural Characterization
- Heparin-Induced Thrombocytopenia and Thrombosis
St. James's Hospital
1988-2025
Sunderland Royal Hospital
2022-2025
Dublin City University
2014-2021
Beaumont Hospital
1990-2020
Central Statistics Office
2020
Trinity College Dublin
1986-2018
University College Dublin
1991-2018
Indiana University Bloomington
2015-2017
Hudson Institute
2016
John Wiley & Sons (United States)
2016
Cloned cDNAs have been isolated that encode a variant of hirudin, potent thrombin inhibitor is secreted by the salivary glands medicinal leech, Hirudo medicinalis. This probably corresponds to form has purified from leech heads but differs in amino acid sequence hirudin whole leeches. There are at least three transcripts detectable RNAs different size, site synthesis, inducibility starvation, and relationship activity. The new predicted cDNA heterodisperse transcription products suggest...
To evaluate the contribution of mononuclear phagocytes, and particularly alveolar macrophages, to alpha-1-antitrypsin (alpha 1AT) production in normal alpha 1AT-deficient individuals, Northern analysis with a human 1AT complementary DNA was used demonstrate that messenger RNA (mRNA) can be detected liver, blood monocytes, macrophages. Quantification mRNA expression demonstrated that: (a) type PiMM monocytes macrophages expressed, respectively, 200-fold 70-fold less per cell than liver; (b)...
Current concepts relating to the pathogenesis of emphysema associated with cigarette smoking is that an imbalance exists within lower respiratory tract between neutrophil elastase and local anti-neutrophil screen, enabling uninhibited destroy alveolar structures over time. The possible role macrophages in contributing this was investigated by evaluating ability smokers' inactivate alpha 1-antitrypsin (alpha 1AT), major human tract. In vitro, smokers spontaneously released 2.5-fold more...
Ad CFTR, a replication-deficient adenovirus expressing the human cystic fibrosis transmembrane conductance regulator (CFTR), was administered by aerosolization in single escalating dose to three pairs (cohorts) of (CF) patients. Buffer only nose and lungs 9–14 days before nasal instillation virus followed day after CFTR lung. Nasal doses (defined terms viral plaque forming units, pfu) were 105, 107, 4 × 108, whereas aerosolized 5.4 108 for each cohort, respectively. No acute toxic effects...
A retroviral vector was used to insert human alpha 1-antitrypsin (alpha 1AT) complementary DNA into the genome of mouse fibroblasts create a clonal population secreting 1AT. After demonstrating that this clone produced 1AT after more than 100 doublings in absence selection pressure, transplanted peritoneal cavities nude mice. When animals were evaluated 4 weeks later, detected both sera and epithelial surface lungs. The could be recovered from those mice demonstrated still producing Thus,...
A cDNA clone containing the complete human alpha 1-antitrypsin sequence was isolated from a liver bank by screening with chemically synthesized oligonucleotide probe. DNA sequences encoding mature polypeptide were inserted into an Escherichia coli expression vector that allows transcription efficient leftward promoter of bacteriophage lambda (PL) and initiation translation at cII gene ribosome-binding site. This construction resulted in induction 45-kilodalton protein level approximately 15%...
Two low molecular weight (LMW) apoproteins were isolated from human pulmonary surfactant. SDS polyacrylamide gel analysis showed one protein (SP 18) to have an apparent of 18,000 when unreduced and 9,000 D after reduction. The second 9) migrated at approximately in the presence or absence reducing agents. Both proteins contain a high number hydrophobic amino acids. NH2-terminal sequence SP 18 was determined be: NH2-phe-pro-ile-pro-leu-pro-tyr-. A cDNA clone adult lung library contained long...
This study reports the entire nucleotide sequence of protein coding region alpha 1-antitrypsin (alpha 1AT) Z gene, a common form 1AT gene associated with serum deficiency. In addition to Glu342 Lys342 mutation in exon V which has been previously identified by peptide analysis, another point (GTG GCG III) predicts second amino acid substitution (Val213 Ala213) protein. Val213 Ala213 was confirmed be general finding type evaluating genomic DNA from 40 haplotypes using synthetic oligonucleotide...
Animal studies indicate that the use of replication-deficient adenovirus for human gene therapy is limited by host antivector immune responses result in transient recombinant protein expression and blocking transfer when rechallenged. Therefore, we have examined to an adenoviral vector beta-galactosidase four patients with lung cancer given a single intratumor injection 10(9) plaque-forming units adenovirus. The was expressed day-8 tumor biopsies from all at variable levels. Recombinant...
EMPHYSEMA is a chronic disorder of the lower respiratory tract characterized by enlargement air space distal to terminal bronchioles resulting from destruction alveolar walls.1 , 2 In United States and Europe, approximately percent cases emphysema are associated with hereditary deficiency α1-antitrypsin,3 4 5 6 52-kd glycoprotein produced hepatocytes and, lesser extent, mononuclear phagocytes.7 8 9 10 11 12 13 14 15 16 17 The single gene coding for α1-antitrypsin contained within 10-kb...
Despite vigorous efforts at curbing tobacco consumption and aggressive combined-modality treatment programs, both the incidence of mortality from lung cancer have remained virtually unchanged in last 10 years. More effective innovative therapies are clearly needed. The direct transfer into tumor cells suppressor genes or toxic gene products that specifically promote cell death spare nonmalignant is a potentially novel anticancer approach should be investigated. Purpose: On basis compelling...
Journal Article The influence of mRNA primary and secondary structure on human IFN-γ gene expression in E. coli Get access Luc-Henri Tessier, Tessier Transgene S.A., 11 Rue de Molsheim, 67000 Strasbourg, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Paul Sondermeyer, Sondermeyer Thérèse Faure, Faure Dominique Dreyer, Dreyer Annie Benavente, Benavente Villeval, Villeval Michael Courtney, Courtney Jean-Pierre Lecocq Nucleic Acids Research, Volume 12,...
al-Antitrypsin (al-AT) deficiency is a hereditary disorder associated with serum al-AT levels ~3 5 % of normal.There are two categories genes that cause this state: the deficient alleles, in which present but low levels, and null no can be attributed to gene.The study defines molecular basis for gene nulbrDnireFalb, identified cloned from genomic DNA an individual severe emphysema resulting heterozygous inheritance nulbraniteFdb Z genes.Sequencing 6"flanking region, all five coding exons,...
The S-type α1-antitrypsin (α1AT) deficiency allele differs from the normal M1(Val213) by a single amino acid substitution (Glu264 → Val). To evaluate molecular pathophysiology responsible for reduced serum levels of al AT associated with allele, α1AT gene expression was examined in blood monocytes, cells which normally produce α1AT, as well murine fibroblasts modified retroviral transfer to express and M-type human genes. Northern analysis S1 protection demonstrated that monocytes M S...
The increasingly ubiquitous nature of online learning has necessarily changed pedagogical approaches in the 21st century. This article will present an overview evolution distance with a particular emphasis on current models and emerging methods instruction for learners. authors detail how various management systems tools as well objects can be used mechanisms creating platform student investment ownership process. Additional discussion focus these enhance teaching, training, peer...
This study investigates the relationship between white matter hyperintensities (WMHs) and longitudinal cognitive decline in older adults. Using data from The Irish Longitudinal Study on Ageing (TILDA), we examined WMH characteristics, including volume, location, microstructural integrity, a community-dwelling population of 497 individuals over six-year period. WMHs were categorised into phenotypes based their size, fractional anisotropy (FA), mean diffusivity (MD), with subtypes for...
Homozygous inheritance of the Z-type mutant form α1-antitrypsin (α1AT) gene results in most common α1AT deficiency, a human hereditary disease associated with high risk for development emphysema and an increased incidence neonatal hepatitis. The α1AT-synthesizing cells individuals Z have normal messenger RNA levels, but secretion is markedly reduced secondary to accumulation newly synthesized rough endoplasmic reticulum. Crystallographic analysis predicts that α1AT, negatively charged Glu...