A5044453732- Nanoparticle-Based Drug Delivery
- Intraocular Surgery and Lenses
- Advanced Polymer Synthesis and Characterization
- Ocular Surface and Contact Lens
- Cutaneous Melanoma Detection and Management
- Alkaloids: synthesis and pharmacology
- Traditional and Medicinal Uses of Annonaceae
- Marine animal studies overview
- Mercury impact and mitigation studies
- Advanced Proteomics Techniques and Applications
- RNA Interference and Gene Delivery
- Mass Spectrometry Techniques and Applications
- Bioactive Natural Diterpenoids Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Bone Tissue Engineering Materials
- Cancer therapeutics and mechanisms
- Synthesis and bioactivity of alkaloids
- Estrogen and related hormone effects
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Melanoma and MAPK Pathways
- Plant-based Medicinal Research
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Corneal surgery and disorders
University of Liège
2010-2020
Cell Culture Company (United States)
2015
Centre de Recherches sur les Macromolécules Végétales
2015
University of Mons
2007
University of Antwerp
2002
Rega Institute for Medical Research
2002
Inserm
1999-2002
Institut de Chimie
1999
Laboratoire de Chimie
1999
In this study, we report the synthesis of a nanoscaled drug delivery system, which is composed gold nanorod‐like core and mesoporous silica shell (GNR@MSNP) partially uploaded with phase‐changing molecules (1‐tetradecanol, TD, T m 39 °C) as gatekeepers, well its ability to regulate release doxorubicin (DOX). Indeed, nearly zero premature evidenced at physiological temperature (37 °C), whereas DOX efficiently achieved higher not only upon external heating, but also via internal heating...
On the basis of original lead neocryptolepine or 5-methyl-5H-indolo[2,3-b]quinoline, an alkaloid from Cryptolepis sanguinolenta, derivatives were prepared using a biradical cyclization methodology. Starting easily accessible educts, this approach allowed synthesis hitherto unknown compounds with varied substitution pattern. As result steric hindrance, preferential formation 3-substituted isomers over 1-substituted was observed when cyclizing...
Matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is a powerful technique giving access to the distribution of large range biomolecules directly from tissue section, allowing, for example, discovery new pathological biomarkers. Nevertheless, one main difficulty lies in identification detected species, especially proteins. MALDI-in source decay (ISD) used fragment ions mass spectrometer ion source. This does not require any special sample treatment but only use...
Mercury is known to bioaccumulate and magnify in marine mammals, which a cause of great concern terms their general health. In particular, the immune system be susceptible long-term mercury exposure. The aims present study were (1) determine level blood free-ranging harbour seals from North Sea (2) examine link between methylmercury vitro exposure functions using seal human mitogen-stimulated peripheral mononuclear cells (T-lymphocytes). Total was analysed 22 seals. Peripheral isolated (n =...
Core-shell nanoparticles made of a maghemite core and mesoporous silica shell were developed as drug delivery systems (DDS). Doxorubicin® (DOX, DNA intercalating drug) was loaded within the cavities, while phase-change molecules (PCMs), e.g. 1-tetradecanol (TD) with melting temperature (Tm) 39 °C, introduced gatekeepers to regulate release behaviours. An overall loading amount ca. 20 wt% (TD/DOX 50/50 wt/wt) confirmed. Heat-triggered DOX evidenced "zero premature release" (<3% entire payload...
Cryptolepine, matadine, and serpentine are three indoloquinoline alkaloids isolated from the roots of African plants: Cryptolepis sanguinolenta, Strychnos gossweileri, Rauwolfia serpentina, respectively. For a long time, these have been used in folk medicine form plant extracts for treatment multiple diseases, particular as antimalarial drugs. To date, molecular basis their diverse biological effects remains poorly understood. elucidate mechanism action, we studied interaction with DNA on...
Cataract surgery is a routine ophthalmologic intervention resulting in replacement of the opacified natural lens by polymeric intraocular (IOL). A main postoperative complication, as result protein adsorption and epithelial cell (LEC) adhesion, growth, proliferation, secondary cataract, referred to posterior capsular opacification (PCO). To avoid PCO formation, poly(ethylene glycol) (PEG) chemical coating was created on surface hydrogel IOLs. Attenuated total reflectance Fourier transform...
Poly(ethylene glycol)-b-poly(N-vinylcaprolactam) (PEG-b-PNVCL) copolymers are prepared from a PEG macro-chain transfer agent in DMF at 65 °C via reversible addition–fragmentation chain (RAFT) polymerization. The well-defined PEG114-b-PNVCL237 copolymer with cloud point temperature of 39 is used for the formation thermo-responsive polymer corona on surface gold nanorods (GNRs) “grafting-to” approach. Thermo-responsiveness and thermo-dependent optical properties as-obtained GNR@PEG-b-PNVCL...
Reversibly crosslinked (RCL) nanogels made of thermo-responsive poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) copolymers were combined with maghemite nanoparticles and developed as new drug delivery systems (DDS). The crosslinking was formed via boronate/diol bonding from the surface-functionalized superparamagnetic nanoparticles, endowing DDS thermo-, pH- glucose-responsiveness. capability to load a hydrophobic model Nile red (NR) within RCL evaluated, stimuli-triggered release behaviours...
Despite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or early CRC stages remains challenging is important for reducing incidence increasing patient’s survival. We analysed 76 tissue samples originated from stages, normal inflamed mucosa by label-free proteomics. The characterisation three selected biomarker candidates was performed immunohistochemistry on an independent set precancerous...
The solution behaviour in water of a polyoxazoline-type block copolymer above the LCST poly(2-isopropyl-2-oxazoline) was exploited to induce temporary or permanent self-assembly.
The antifouling properties of poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) hydrogels were improved by the surface grafting a brush poly(oligoethylene glycol methyl ether acrylate) [poly(OEGA)]. atom-transfer radical polymerization (ATRP) OEGA (degree = 8) was initiated from preactivated hydrogel under mild conditions, thus in water at 25 °C. catalytic system optimized on basis two ligands [1,1,4,7,10,10-hexamethyl-triethylenetetramine (HMTETA) or tris[2-(dimethylamino)ethyl]amine...
Hydrogels and nanogels made from partially hydrolyzed poly(2-ethyl-2-oxazoline) (PEtOx) were prepared by two different processes. First, both hydrogels in dilute media the presence of 1,6-hexanediol di-glycidyl ether (1) as cross-linker. Alternatively, PEtOx precursor was engineered into an inverse w/o emulsion process. In latter case, addition to cross-linker 1, a cleavable homologue (2), namely 1,6-hydroxyethyl disulfide-bis-di-glycidyl ether, used produce nanogels. The pH-responsiveness...
Reversibly crosslinked thermo- and redox-responsive PVOH-<italic>b</italic>-PNVCL nanogels designed for controlled drug release.
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a rapidly growing method in biomedical research allowing molecular mapping of proteins on histological sections. The images can be analyzed terms spectral pattern to define regions interest. However, the identification and differential quantitative analysis require off line or situ proteomic methods using enzymatic digestion. rapid biomarkers holds great promise for diagnostic research, but major obstacle...
Abstract Herein, we report a new drug‐delivery system (DDS) that is comprised of near‐infrared (NIR)‐light‐sensitive gold‐nanorod (GNR) core and phase‐changing poly( ε ‐caprolactone) ‐b‐ poly(ethylene glycol) polymer corona (GNR@PCL PEG). The underlying mechanism the drug‐loading triggered‐release behaviors involves entrapment drug payloads among PCL crystallites heat‐induced phase change, respectively. A low premature release pre‐loaded doxorubicin was observed in PBS buffer (pH 7.4) at 37...
Original core/corona nanoparticles composed of a maghemite core and stimuli-responsive polymer coating made poly(acrylic acid)-block-poly(vinyl alcohol) macromolecules were fabricated for drug delivery system (DDS) application. This kind DDS aims to combine the advantage coating, in order regulate release behaviours under different conditions furthermore, improve biocompatibility vivo circulation half-time nanoparticles. Drug loading capacity was evaluated with methylene blue (MB), cationic...
Posterior Capsular Opacification (PCO) is the capsule fibrosis developed on implanted IntraOcular Lens (IOL) by de-differentiation of Epithelial Cells (LECs) undergoing Mesenchymal Transition (EMT). Literature has shown that incidence PCO multifactorial including patient's age or disease, surgical technique, and IOL design material. Reports comparing hydrophilic hydrophobic acrylic IOLs have former more severe PCO. On other hand, we previously demonstrated adhesion LECs favored compared to...