A5027464593- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Pluripotent Stem Cells Research
- Memory and Neural Mechanisms
- Pain Mechanisms and Treatments
- Advanced Memory and Neural Computing
- CRISPR and Genetic Engineering
- Neurogenesis and neuroplasticity mechanisms
- Developmental Biology and Gene Regulation
- Renin-Angiotensin System Studies
- Optical Polarization and Ellipsometry
- Phosphodiesterase function and regulation
- Molecular Communication and Nanonetworks
- Exercise and Physiological Responses
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
- Bone Metabolism and Diseases
- EEG and Brain-Computer Interfaces
- Medicinal Plant Pharmacodynamics Research
- Neuropeptides and Animal Physiology
- Neurobiology and Insect Physiology Research
- Hereditary Neurological Disorders
- Nicotinic Acetylcholine Receptors Study
Aarhus University
2020-2025
Danish National Research Foundation
2021-2025
Inserm
2016-2021
Université Clermont Auvergne
2021
Danish Diabetes Academy
2020
Neuro-Dol
2016-2018
Clermont Université
2016
A well-known phenomenon in the optogenetic toolbox is that all light-gated ion channels, including red-shifted channelrhodopsins (ChRs), are activated by blue light, whereas blue-shifted ChRs minimally responsive to longer wave-lengths. Here, we took advantage of this feature create a system which allows high-frequency activation neurons with pulses red while permitting suppression action potentials millisecond precision light. We achieved pairing an ultrafast ChR light-sensitive anion...
Mechanical allodynia, a widespread pain symptom that still lacks effective therapy, is associated with the activation of dorsally directed polysynaptic circuit within spinal dorsal horn (SDH) or medullary (MDH), whereby tactile inputs into deep SDH/MDH can gain access to superficial SDH/MDH, eliciting pain. Inner lamina II (II i ) interneurons expressing γ isoform protein kinase C (PKCγ + are key elements for allodynia circuits, but how they operate unclear. Combining behavioral, ex vivo...
Behavioral flexibility and timely reactions to salient stimuli are essential for survival. The subcortical thalamic-basolateral amygdala (BLA) pathway serves as a shortcut ensuring rapid processing. Here, we show that BLA neuronal thalamic axonal activity in mice mirror the defensive behavior evoked by an innate visual threat well auditory learned threat. Importantly, perturbing this compromises responses both forms of threats, animals fail switch from exploratory behavior. Despite shared...
The differentiation of patient-specific induced pluripotent stem cells (iPSCs) into specific neuronal subtypes has been exploited as an approach for modeling a variety neurological disorders. However, achieving highly pure population neurons is challenging when using directed methods, especially generated by complex and protracted protocols. In this study, we efficiently produced populations regionally specified CNS modified NGN2-Puromycin direct conversion protocol. protocol amenable across...
Current differentiation protocols for generating mesencephalic dopaminergic (mesDA) neurons from human pluripotent stem cells result in grafts containing only a small proportion of mesDA when transplanted vivo. In this study, we develop lineage-restricted undifferentiated (LR-USCs) cells, which enhances their potential differentiating into caudal midbrain floor plate progenitors and neurons. Using ventral protocol, 69% LR-USCs become bona fide progenitors, compared to 25% embryonic (hESCs)....
A fundamental interest in circuit analysis is to parse out the synaptic inputs underlying a behavioral experience. Toward this aim, we have devised an unbiased strategy that specifically labels afferent are activated by defined stimulus activity-dependent manner. We validated four brain areas receiving known sensory inputs. This strategy, as demonstrated here, accurately identifies these
A well-known phenomenon in the optogenetic toolbox is that all light-gated ion channels, including red-shifted channelrhodopsins (ChRs), are activated by blue light, whereas blue-shifted ChRs minimally responsive to longer wave-lengths. Here, we took advantage of this feature create a system which allows high-frequency activation neurons with pulses red while permitting suppression action potentials millisecond precision light. We achieved pairing an ultrafast ChR light-sensitive anion...
Protein kinase C gamma (PKCγ) interneurons, located in the superficial spinal (SDH) and medullary dorsal horns (MDH), have been shown to play a critical role cutaneous mechanical hypersensitivity. However, thorough characterization of their development MDH is lacking. Here, it that number PKCγ-ir interneurons changes from postnatal day 3 (P3) P60 (adult) such developmental differ according laminae. are already present at P3-5 laminae I, IIo, III. In lamina III, they then decrease P11-P15...
A fundamental challenge in the optogenetic toolbox is that all opsins, regardless of their excitation spectra, are activated by blue light. We hypothesized pairing a red-shifted channelrhodopsin with light-sensitive anion channel appropriately matching kinetics shall render neurons responsive to red but not To achieve this, we used semi-rational mutagenesis strategy optimize and light spectrum chloride channelrhodopsin. By optimized variants ZipACR vfChrimson, fast channelrhodopsin, created...
Behavioral flexibility and timely reactions to salient stimuli are essential for survival. The subcortical thalamic-basolateral amygdala (BLA) pathway serves as a shortcut ensuring rapid processing. Here, we show that BLA neuronal thalamic axonal activity mirror the defensive behavior evoked by an innate visual threat well auditory learned threat. Importantly, perturbing this compromises responses both forms of threats, in animals fail switch from exploratory behavior. Despite shared between...
Abstract Pain processing in young mammals is immature. Despite the central role of medullary dorsal horn (MDH) orofacial sensory information, maturation neurons within MDH has been largely overlooked. Combining vitro electrophysiological recordings and 3D morphological analysis over first postnatal month rats, we investigated age-dependent development inner lamina II (IIi) MDH. We show IIi neuronal population transition into a more hyperpolarized state, with modification action potential...
Abstract The differentiation of human pluripotent stem cells (hPSCs) into mesencephalic dopaminergic (mesDA) neurons requires a precise combination extrinsic factors that recapitulates the in vivo environment and timing. Current methods are capable generating authentic mesDA after long-term culture vitro ; however, when progenitors transplanted , resulting only minor components graft. This low yield hampers broad use these clinic. In this study, we genetically modified to generate novel type...
A fundamental challenge in the optogenetic toolbox is that all opsins, regardless of their excitation spectra, are activated by blue light. We hypothesized pairing a red-shifted channelrhodopsin with light-sensitive anion channel appropriately matching kinetics shall render neurons responsive to red but not To achieve this, we used semi-rational mutagenesis strategy optimize and light spectrum chloride channelrhodopsin. By optimized variants ZipACR vfChrimson, fast channelrhodopsin, created...
Abstract A fundamental interest in circuit analysis is to parse out the synaptic inputs underlying a behavioral experience. Toward this aim, we have devised an unbiased strategy that specifically labels afferent are activated by defined stimulus activity-dependent manner. We validated four brain areas receiving known sensory inputs. This strategy, as demonstrated here, accurately identifies these