A5091483935- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Burn Injury Management and Outcomes
- Alcoholism and Thiamine Deficiency
- Trauma and Emergency Care Studies
- Wound Healing and Treatments
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Plant pathogens and resistance mechanisms
- Ecology and Conservation Studies
- Microbial Fuel Cells and Bioremediation
- Disaster Response and Management
- Injury Epidemiology and Prevention
- Genomic variations and chromosomal abnormalities
- Microbial Community Ecology and Physiology
Massachusetts General Hospital
2014-2015
Harvard University
2014-2015
Shriners Hospitals for Children - Boston
2014-2015
Monterey Bay Aquarium Research Institute
2014
Virginia Tech
2007-2013
Recently, genome sequencing of many isolates genetically monomorphic bacterial human pathogens has given new insights into pathogen microevolution and phylogeography. Here, we report a genome-based micro-evolutionary study plant pathogen, Pseudomonas syringae pv. tomato. Only 267 mutations were identified between five sequenced in 3,543,009 nt analyzed sequence, which suggests recent evolutionary origin this pathogen. Further analysis with genome-derived markers 89 world-wide showed that...
Although there are adequate DNA sequence differences among plant-associated and plant-pathogenic bacteria to facilitate molecular approaches for their identification, identification at a taxonomic level that is predictive of phenotype challenge. The problem the absence taxonomy describes genetic variation biologically relevant resolution database containing reference strains comparison. Moreover, evolution, population genetics, ecology, epidemiology many still poorly understood. To address...
Diverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those governing pathogen-host specificity. Comparative genomics of closely related pathogens different host specificity represents an excellent approach for identification genes to host-range determination. A...
Vitamin B1 (thiamine pyrophosphate, TPP) is essential to all life but scarce in ocean surface waters. In many bacteria and a few eukaryotic groups thiamine biosynthesis genes are controlled by metabolite-sensing mRNA-based gene regulators known as riboswitches. Using available genome sequences transcriptomes generated from ecologically important marine phytoplankton, we identified 31 new These were found alveolate, cryptophyte, haptophyte rhizarian phytoplankton well taxa two lineages...
ABSTRACT Pseudomonas syringae pv. tomato strain DC3000 (PtoDC3000) is one of the most intensively studied bacterial plant pathogens today. Here we report a thorough investigation into PtoDC3000 and close relatives isolated from Antirrhinum majus (snapdragon), Apium graveolens (celery), Solanaceae Brassicaceae species. Multilocus sequence typing (MLST) was used to resolve precise phylogenetic relationship between isolates determine importance recombination in their evolution. MLST data were...
Pseudomonas syringae causes plant diseases, and the main virulence mechanism is a type III secretion system (T3SS) that translocates dozens of effector proteins into cells. Here we report existence subgroup P. isolates do not cause disease on any species tested. This group monophyletic most likely evolved from pathogenic ancestor through loss T3SS. In nonpathogenic isolate 508 genomic region in strains contains hrp-hrc cluster coding for T3SS flanking genes absent. was also surveyed presence...
In Brief Objective: To develop predictive models for early triage of burn patients based on hypersusceptibility to repeated infections. Background: Infection remains a major cause mortality and morbidity after severe trauma, demanding new strategies combat Models infection prediction are lacking. Methods: Secondary analysis 459 (≥16 years old) with 20% or more total body surface area burns recruited from 6 US centers. We compared blood transcriptomes 180-hour cutoff the...
Comparative genomics of closely related pathogens that differ in host range can provide insights into mechanisms host-pathogen interactions and adaptation. Furthermore, sequencing multiple strains with the same reveals information concerning pathogen diversity molecular basis virulence. Here we present a comparative analysis draft genome sequences for four Pseudomonas cannabina pathovar alisalensis (Pcal), which is pathogenic on monocotyledonous dicotyledonous plants. These foundation...