A5103280207- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Traditional Chinese Medicine Analysis
- Pharmacological Effects of Natural Compounds
- Ginseng Biological Effects and Applications
- Cancer-related molecular mechanisms research
- Asthma and respiratory diseases
- Plant-based Medicinal Research
- MicroRNA in disease regulation
- Phytochemistry and Biological Activities
- Antiplatelet Therapy and Cardiovascular Diseases
- Respiratory and Cough-Related Research
- Liver Disease Diagnosis and Treatment
- Muscle metabolism and nutrition
- Circular RNAs in diseases
- Adipose Tissue and Metabolism
- Natural product bioactivities and synthesis
- Exercise and Physiological Responses
- Atherosclerosis and Cardiovascular Diseases
- Magnolia and Illicium research
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Bioactive Natural Diterpenoids Research
- Medicinal Plants and Bioactive Compounds
Chinese People's Liberation Army
2025
Ruijin Hospital
2024
Jiangnan University
2024
Medical University of Vienna
2007-2023
Vienna General Hospital
2012-2023
Qinghai University
2023
Beijing Luhe Hospital Affiliated to Capital Medical University
2016-2022
Soochow University
2019
Changzhou No.2 People's Hospital
2019
University of Vienna
2015
Parvalbumins are the most important fish allergens. Polysensitization to various species is frequently reported and linked cross-reactivity of their parvalbumins. Studies on its association allergenicity purified natural parvalbumins from different still lacking. In addition, some studies indicate that dark muscled such as tuna less allergenic.Total protein extracts cod, whiff, swordfish, all eaten in Spain, were tested for IgE-binding properties with 16 allergic patients' sera Madrid. The...
High platelet reactivity and gastric mucosal injury after aspirin (ASA) treatment are associated with poor compliance an increased risk of cardiovascular events. Panax notoginseng saponins (PNS) have been widely used for the coronary heart disease (CHD) in addition to antiplatelet drugs China; however, joint effect possible mechanism PNS ASA on activation remain unclear. This study was designed investigate combinational effects ASA, explore underlying via arachidonic acid (AA) metabolism...
A total of 112 carriers (51 males and 61 females) with Robertsonian translocations who underwent 148 cycles preimplantation genetic testing for structural rearrangements (PGT-SR) between February 2017 December 2023 at the First Center General Hospital Chinese PLA were recruited. The heterologous type rob (13; 14) was most common form translocation, which accounted 65.2% (73/112). euploidy rate blastocysts 42.1% (303/720). Male had higher euploid embryo than female [50.0% (149/298) vs 36.5%...
Terpenoids are valuable chemicals used across industries. Methanol, a nonsugar-based feedstock, offers an eco-friendly approach to terpenoid production. In this study, hybrid methylotrophic pathway was engineered in Serratia marcescens (S. marcescens) HBQA7, which is tolerant both methanol and compounds. The utilizes bacterial dehydrogenase (Mdh) yeast dihydroxyacetone synthase (Das) produce monoterpenes sesquiterpenes from xylose. 13C labeling experiments identified the optimal enzyme pair:...
<i>Background:</i> Individual amino acid residues of the major birch pollen allergen, Bet v 1, have been identified to be crucial for IgE recognition. The objective present study was evaluate whether this concept applicable 1-homologous apple Mal d 1. <i>Methods:</i> A 1 five-point mutant produced by PCR techniques, cloned into pMW 172 and expressed in <i>Escherichia coli</i> BL21(DE3) cells. To allergenic properties engineered protein compared wild-type...
Summary Background Allergen‐specific immunotherapy for food allergy has been hindered by severe side‐effects in the past. Well‐characterized hypo‐allergenic recombinant allergens potentially offer a safe solution. Objective To demonstrate hypo‐allergenicity of mutated major allergen from apple, Mal d 1, vitro and vivo . Methods A mutant apple allergen, was obtained site‐directed mutagenesis exchanging five amino acid residues. Fourteen patients with combined birch pollen‐related were...
<i>Background:</i> Mugwort (<i>Artemisia vulgaris)</i> represents an important source of weed pollen allergens. The objectives the present study were (i) to analyze IgE binding profiles in a group mugwort-allergic patients, (ii) identify individual marker allergens crucial for diagnosis mugwort allergy and (iii) potential crossreactive ragweed (<i>Ambrosia artemisiifolia)</i> extract. <i>Methods:</i> Sera from 100 pediatric patients analyzed...
Allergic reaction following fish consumption can trigger life-threatening reactions in predisposed individuals. Parvalbumins from different species have been identified as the major allergens. There are two distinct phylogenetic lineages of parvalbumins, alpha and beta. Most allergic caused by β-parvalbumins. We cloned expressed cDNAs encoding cod (Gadus morhua) carp (Cyprinus carpio) β-parvalbumins purified natural β-parvalbumin. CD spectra proteins showed that their overall secondary...
Radiotherapy has been widely used for the clinical management of esophageal squamous cell carcinoma. However, radioresistance remains a serious concern that prevents efficacy carcinoma ( ESCC ) radiotherapy. REV 7, structural subunit eukaryotic DNA polymerase ζ, multiple functions in bypassing damage and modulating mitotic arrest human lines. expression molecular function 7 progression unclear. In this study, we first examined samples, found higher tissues compared to matched adjacent or...
Fatty acid translocase CD36 (CD36) plays an important role in the initiation and pathogenesis of chronic liver disease non-alcoholic fatty (NAFLD). The purpose this study is to investigate regulation microRNA-20a-5p (miR-20a-5p) on NAFLD. Human plasma samples were obtained from NAFLD patients healthy controls. Mice fed with high-fat diet induce vivo model. Histology staining was performed examine morphology lipid deposition mouse tissue. Real-time PCR, dual-luciferase assay, western blotting...
Apple (Malus domestica) is the most widely cultivated fruit crop in Europe and frequently causes allergic reactions with a variable degree of severity. So far, four apple allergens Mal d 1, 2, 3 4 have been identified. Bet v 1 related allergen, 4, profilin, are sensitive to proteolytic degradation, whereas thaumatin-like protein 3, nonspecific lipid transfer protein, rather stable processes. were purified after expression Escherichia coli system, while 2 from tissue. All proteins subjected...
Abstract Background Profilins are ubiquitous panallergens that have been extensively characterized; yet, their clinical relevance is still unclear. Objective The aim of the present study was to produce recombinant apple profilin (rMal d 4) and evaluate its allergenic activity potency for component‐resolved allergy diagnosis. Methods Complementary DNA‐derived Mal 4 cloned, expressed in Escherichia coli subsequently purified via poly ( l ‐proline) sepharose. A total 28 sera from apple‐allergic...
<i>Background:</i> Assessment of allergenicity foods is important for allergic consumers and regulators. Immunoassays to measure major food allergens are widely applied, often giving variable results. Using the apple allergen Mal d 1 as a model, we aimed establish at molecular level why different immunoassays assessing cultivars produce conflicting outcomes. <i>Methods:</i> was measured in 53 from Italy 35 The Netherlands, using four immunoassays. Purified standards...
Whiff (Lepidorhombus whiffiagonis) is a fish frequently consumed in Spain. Lep w 1, its major allergen, calcium-binding beta-parvalbumin. The resistance of 1 to heat denaturation and digestion were studied by circular dichroism spectroscopy vitro gastric systems. Purified was thermally stable up 65 degrees C at neutral pH. Calcium depletion resulted change structure as determined spectroscopy. A partial loss also observed acidic pH; however, the allergen retained full IgE-binding ability....
The aim of this study was to evaluate the anti-inflammatory profiling Chinese herbal formula Sini Tang (SNT) in myocardial infarction (MI) rats. SNT, a decoction consisting four herbs: Aconitum carmichaelii, Cinnamomum cassia, Zingiber officinale, and Glycyrrhiza uralensis, characterized as remedy treat syndromes corresponding heart failure MI China. Potential biomarkers, which reflect extent necrosis correlate with cardiac outcomes following MI, such atrial natriuretic peptide (ANP), high...
The gut microbiota plays a crucial role in maintaining host nutrition, metabolism, and immune homeostasis, particularly extreme environmental conditions. However, the regulatory mechanisms of animal organisms hypobaric hypoxia exposure require further study. We conducted research by comparing SD rats treated with an antibiotic (ABX) cocktail untreated that were housed low-pressure oxygen chamber (simulating low pressure hypoxic environment at 6000 m altitude) for 30 days. After experiment,...
(-)-α-Bisabolol exhibits analgesic, anti-inflammatory, and skin-soothing properties is widely applied in the cosmetic pharmaceutical industries. The use of plant essential oil distillation or chemical synthesis to produce (-)-α-bisabolol both inefficient unsustainable. Currently, microbial production mainly relies on
Prostate carcinoma is the second most common cancer among men worldwide. Developing new therapeutic approaches and diagnostic biomarkers for prostate (PC) a significant need. The Chinese herbal medicine